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    Wyświetlanie 1-3 z 3
    Tytuł:
    Species-specific chloroplast DNA polymorphism in the trnV-rbcL region in Pinus sylvestris and P. mugo
    Autorzy:
    Wachowiak, W
    Baczkiewicz, A.
    Celinski, K.
    Prus-Glowacki, W.
    Powiązania:
    https://bibliotekanauki.pl/articles/41357.pdf
    Data publikacji:
    2004
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    Scotch pine
    Pinus sylvestris
    Pinus mugo
    dwarf pine
    hybridization
    DNA marker
    mtDNA
    trnV-rbcL region
    chloroplast
    DNA polymorphism
    Opis:
    Four cpDNA regions were analyzed with the use of PCR-RFLP technique and nucleotide sequences of two mtDNA regions were characterized in order to find P. sylvestris and P. mugo species specific markers useful for studies of the species hybridization. The difference in the restriction fragment patterns of trnV-rbcL region after digestion with MvaI endonuclease was detected. The analyses of the species representatives from various geographic regions revealed that the observed polymorphism is species specific. No differences have been disclosed in the analyzed trnS-trnT, trnK1-trnK2, trnC-trnD cpDNA regions. The P. sylvestris and P.mugo mtDNA sequences of orf25 and coxI regions proved to be identical.
    Źródło:
    Dendrobiology; 2004, 51; 67-72
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Genetic variation of Picea abies in southern Germany as determined using isozyme and STS markers
    Autorzy:
    Konnert, M
    Powiązania:
    https://bibliotekanauki.pl/articles/40969.pdf
    Data publikacji:
    2009
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    international conference
    Europe
    forest ecosystem
    plant breeding
    tree
    Norway spruce
    Picea abies
    genetic variation
    Germany
    isoenzyme
    Bavaria
    DNA marker
    plant genetics
    provenance
    Opis:
    Over 50 populations of Norway spruce from Bavaria were analysed at 23 isozyme gene loci. The mean genetic distances between these populations were quite small. A geographical grouping could not be observed, and discrimination between provenances from high and low altitudes was not identifiable using this marker type, either. The only difference between spruce populations from South Bavaria and those from Northeast Bavaria is in the presence of some distinct rare alleles. The highest values for the genetic diversity were detected for spruce stands in Northeast Bavaria (Frankonian Forest). Using STS markers, further genes of the nuclear genome of Picea abies can be dealt with. The genetic differences found on the basis of ten STS markers between different Picea abies seed lots and/or seedling populations are generally 2-3 times greater than those found by means of isozyme gene markers. DNA markers turned out to be an appropriate and substantial addition or even more a suitable alternative to isozyme markers for analysing genetic variation and testing provenance identity. Their advantages consist in a markedly wider variation as well as in the enlarged genome segments investigated.
    Źródło:
    Dendrobiology; 2009, 61 Supplement
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Mitochondrial and nuclear DNA differentiation of Picea abies populations in Poland
    Autorzy:
    Nowakowska, J A
    Powiązania:
    https://bibliotekanauki.pl/articles/40976.pdf
    Data publikacji:
    2009
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    international conference
    Europe
    forest ecosystem
    plant breeding
    tree
    Norway spruce
    Picea abies
    Polska
    mitochondrial DNA
    nuclear DNA
    plant population
    microsatellite marker
    post-glacial migration
    Opis:
    The natural stands of Norway spruce in Poland are split between the southern and the northeastern parts of the country. Two so-called "spruceless" zones separate the northern spruce locations from those in the south, one "spruceless" zone is situated in Central Poland, and the other one in the Beskid Mts. Mitochondrial (STS) and nuclear (SSR) markers were used to perform the genetic identification of Norway spruce. Four different variants of haplotypes, "a", "b", "c" and "d", were found to occur in the nad1 locus of STS markers. Populations from the northern range of Picea abies distribution in Poland harboured exclusively haplotypes "c" and "d", except for the Białowieża population which had haplotypes "a" and "c". Populations from the "spruceless" zones contained four types of haplotypes whilst those from southern Poland were mostly composed of haplotype "a". High mean gene diversity was observed for both STS and SSR markers (HT = 0.529, and HT = 0.851, respectively). The total genetic differentiation of Norway spruce populations was very low (FST= 0.088). Two main groups of populations were distinguished in the dendrogram defined by Nei's genetic distances based on microsatellite markers. The distribution of the genotypes was scattered and did not show any connection with the spatial distribution of P. abies in Poland. Only the mtDNA markers were able to differentiate the northern populations of Norway spruce from the southern ones, proving the historical separation between the Baltico-Nordic and the Hercyno-Carpathian ranges of P. abies in Poland. By contrast, the microsatellite data suggested an overlap between the genotypes due to the human manipulation of Norway spruce stands in the past.
    Źródło:
    Dendrobiology; 2009, 61 Supplement
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-3 z 3

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