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    Wyświetlanie 1-3 z 3
    Tytuł:
    New microfluidic device for lactate dehydrogenase (LDH) activity analysis
    Autorzy:
    Jędrych, E.
    Mazur, M.
    Grabowska-Jadach, I.
    Brzózka, Z.
    Powiązania:
    https://bibliotekanauki.pl/articles/115778.pdf
    Data publikacji:
    2012
    Wydawca:
    Fundacja na Rzecz Młodych Naukowców
    Tematy:
    lactate dehydrogenase (LDH) activity
    microfluidic system
    PDMS
    adherent cell culture
    cytotoxicity analysis
    Opis:
    In this paper, we present cytotoxicity analysis (determination of lactate dehydrogenase — LDH activity performed in a designed and fabricated microfl uidic system. This method allowed for analysis of a supernatant collected from A549 (human lung cancer) and HT-29 (human colon cancer epithelial) cells, which were incubated for 24 h with selected compounds. LDH is an intracellular enzyme present in tissues, which is released into the supernatant caused by membrane damage or cell lyses. In our tests, LDH-Cytotoxicity Assay Kit (BioVision) was used. The toxic eff ect of drugs was measured in the developed microsystem made of PDMS (poly(dimethylsiloxane)). Analytical reaction took place in the special designed microchannel geometry. Then, the LDH activity was measured at 490 nm using spectrophotometer. In subsequent experiments, appropriate conditions for measurements using a microfl uidic system were chosen. It was found that the selected reagent is sensitive to temperature changes and light exposure. Reaction time in the microsystem was determined by changes of fl ow rates of reagents. Afterwards, for the various reaction time, the toxic eff ect of 5-fl uorouracil, celecoxib and 1,4-dioxane was performed. The obtained results were compared with the results carried out in 96-well plates. Based on these results, it was noted that the enzymatic reaction time in the microsystem is shorter than in 96-well plate. Moreover, the advantage of using microsystem is also the small amount of reagents.
    Źródło:
    Challenges of Modern Technology; 2012, 3, 4; 3-8
    2082-2863
    2353-4419
    Pojawia się w:
    Challenges of Modern Technology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    ‘Lab-on-a-chip’ for cell engineering: towards cellular models mimicking in vivo
    Autorzy:
    Ziółkowska, K.
    Chudy, M.
    Dybko, A.
    Brzózka, Z.
    Powiązania:
    https://bibliotekanauki.pl/articles/115885.pdf
    Data publikacji:
    2011
    Wydawca:
    Fundacja na Rzecz Młodych Naukowców
    Tematy:
    Lab-on-a-chip
    microfluidic tools
    cancer
    cell culture
    Multicellular Tumor Spheroid (MCTS)
    Opis:
    One of the main scopes of modern cell engineering is development of cellular models that can replace animals in drug screening and toxicological tests, so called alternative methods. Construction of the alternative model is a very challenging task due to a richness of factors creating the in vivo environment. The monolayer cell culture — cultivation of adhesive cells on artificial surfaces such as glass or polymer — lack most of the in vivo-like interactions, but still is the only tool for the majority of applications. One of the most prospective approaches on mimicking in vivo environment is “Lab-on-a-chip” technology. Microfluidic devices offer lots of advantages over traditional in vitro culture, e.g. much higher cell volume-to-extracellular fluid volume ratio or possibility of regulation of hydrodynamic stress. This presentation aims to introduce latest advances of our team in microfluidic cell culture devices. Our novel approach is to cultivate three dimensional multicellular aggregates (spheroids) in microenvironments arranged in a microfluidic system. The geometry and materials of the system allow for cultivation, observation and analysis of multicellular spheroids. The results presented concern multicellular tumor spheroids (MCTS) rising from human cancer cells, which are considered to represent most of the conditionings of cancer tumor in vivo. The fully developed MCTS microdevice will be a reliable tool for anticancer drug screening, as the results most likely will be in a close accordance with the results obtained in vivo.
    Źródło:
    Challenges of Modern Technology; 2011, 2, 1; 79-82
    2082-2863
    2353-4419
    Pojawia się w:
    Challenges of Modern Technology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Microfluidic devices — application in anticancer studies
    Autorzy:
    Jędrych, E.
    Chudy, M.
    Dybko, A.
    Brzózka, Z.
    Powiązania:
    https://bibliotekanauki.pl/articles/115929.pdf
    Data publikacji:
    2012
    Wydawca:
    Fundacja na Rzecz Młodych Naukowców
    Tematy:
    microfluidic system
    PDMS
    adherent cell culture
    concentration gradient generator (CGG)
    cytotoxicity tests
    photodynamic therapy (PDT) procedures
    Opis:
    A rapidly growing pharmaceutical industry requires faster and more efficient ways to find and test new drugs. One of the new method for cell culture and examining the toxic effects of drugs is application of microfluidic systems. They provide new types of microenvironments and new methods for investigation of anticancer therapy. The use of microsystems is a solution that gives the opportunity to reduce not only cost and time, but also a number of tests on animals. In this paper we present designed and fabricated hybrid microfluidic systems which are applicable for cell culture, cell based cytotoxicity assays and photodynamic therapy procedures. Polydimethylsiloxane (PDMS) and sodium glass were used for fabrication of microdevices. The designed geometry of the microdevices includes cell culture microchambers and a concentration gradient generator (CGG). The CGG enables to obtain diff erent concentrations of tested drugs in a single step, which is a significant simplification of cytotoxicity assay procedure. In the designed microsystems three various cell lines (normal and carcinoma) were cultured and analyzed.
    Źródło:
    Challenges of Modern Technology; 2012, 3, 2; 3-5
    2082-2863
    2353-4419
    Pojawia się w:
    Challenges of Modern Technology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-3 z 3

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