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Wyświetlanie 1-3 z 3
Tytuł:
Novel designs and technologies for cell engineering
Autorzy:
Ziółkowska, K.
Chudy, M.
Dybko, A.
Brzózka, Z.
Powiązania:
https://bibliotekanauki.pl/articles/115526.pdf
Data publikacji:
2011
Wydawca:
Fundacja na Rzecz Młodych Naukowców
Tematy:
lab-on-a-chip
multicellular tumor spheroid
cancer
in vitro cell culture
cell engineering
Opis:
Microfluidic devices, such as lab-on-a-chip systems, are highly advantageous for cell engineering and cell based assays. It is a particularly useful approach for development of the in vitro cellular systems mimicking the in vivo environment. In this paper, a novel lab-on-a-chip device for three-dimensional human cell culture and anticancer drug testing is presented. Cells were cultured as Multicellular Tumor Spheroids (MCTS) — the best cancer tumor model developed so far. Diff erent designs were tested and novel technique of microfabrication in poly(dimethylsiloxane) was developed. MCTS were cultured in a system of polymeric microwells, with the network of microfluidic channels for culture medium flow. Design included optimal shear stress and proper nutrients supply for cultured cells. Final design provided MCTS culture for four weeks with the homeostasis-like state achievement, which is characteristic for the in vivo situation.
Źródło:
Challenges of Modern Technology; 2011, 2, 4; 54-60
2082-2863
2353-4419
Pojawia się w:
Challenges of Modern Technology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
‘Lab-on-a-chip’ for cell engineering: towards cellular models mimicking in vivo
Autorzy:
Ziółkowska, K.
Chudy, M.
Dybko, A.
Brzózka, Z.
Powiązania:
https://bibliotekanauki.pl/articles/115885.pdf
Data publikacji:
2011
Wydawca:
Fundacja na Rzecz Młodych Naukowców
Tematy:
Lab-on-a-chip
microfluidic tools
cancer
cell culture
Multicellular Tumor Spheroid (MCTS)
Opis:
One of the main scopes of modern cell engineering is development of cellular models that can replace animals in drug screening and toxicological tests, so called alternative methods. Construction of the alternative model is a very challenging task due to a richness of factors creating the in vivo environment. The monolayer cell culture — cultivation of adhesive cells on artificial surfaces such as glass or polymer — lack most of the in vivo-like interactions, but still is the only tool for the majority of applications. One of the most prospective approaches on mimicking in vivo environment is “Lab-on-a-chip” technology. Microfluidic devices offer lots of advantages over traditional in vitro culture, e.g. much higher cell volume-to-extracellular fluid volume ratio or possibility of regulation of hydrodynamic stress. This presentation aims to introduce latest advances of our team in microfluidic cell culture devices. Our novel approach is to cultivate three dimensional multicellular aggregates (spheroids) in microenvironments arranged in a microfluidic system. The geometry and materials of the system allow for cultivation, observation and analysis of multicellular spheroids. The results presented concern multicellular tumor spheroids (MCTS) rising from human cancer cells, which are considered to represent most of the conditionings of cancer tumor in vivo. The fully developed MCTS microdevice will be a reliable tool for anticancer drug screening, as the results most likely will be in a close accordance with the results obtained in vivo.
Źródło:
Challenges of Modern Technology; 2011, 2, 1; 79-82
2082-2863
2353-4419
Pojawia się w:
Challenges of Modern Technology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
An integrated gas removing system for microfluidic application. Design and evaluation
Autorzy:
Hofman, I. M.
Ziółkowska, K.
Dybko, A.
Brzózka, Z.
Powiązania:
https://bibliotekanauki.pl/articles/115931.pdf
Data publikacji:
2013
Wydawca:
Fundacja na Rzecz Młodych Naukowców
Tematy:
gas removing system
debubbler
debubbling system
lab-on-a-chip
microfluidics
long-term cell culture
cell spheroids
HT-29 cells
Opis:
Microfluidic systems are used in a wide range of applications, including medical diagnostics, cell engineering and bioanalytics. In this work we focused on “Lab-on-a-chip” microsystems for cell cultivation. A troublesome problem of gas bubbles entering microdevices causing signal interferences and cells damage was emphasized. A novel, integrated debubbler in the form of cylindrical traps covered with thin PDMS membrane was designed and manufactured. Demonstrated debubbler was successfully applied in a long-lasting culture of HT-29 cell aggregates.
Źródło:
Challenges of Modern Technology; 2013, 4, 3; 10-15
2082-2863
2353-4419
Pojawia się w:
Challenges of Modern Technology
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-3 z 3

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