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Wyświetlanie 1-15 z 56
Tytuł:
Enhancing zinc levels in Solanum lycopersicum L. through biofortification with plant growth-promoting Pseudomonas spp. isolated from cow dung
Autorzy:
Karnwal, Arun
Powiązania:
https://bibliotekanauki.pl/articles/16690272.pdf
Data publikacji:
2023
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
biofortification
plant growth promotion
Pseudomonas
Solanum lycopersicum
zinc
Opis:
Zinc is a vital micronutrient for all life forms, and Zn-solubilizing bacteria (ZSB) present in the soil convert inorganic zinc into forms available for plants. This study assessed ZSB isolated from cow dung for their plant growth-promoting (PGP) characteristics and potential to enhance tomato plant growth. The experiment assayed a total of 30 bacteria from cow dung for Zn-solubilization using insoluble ZnO and ZnCO3. Atomic absorption spectroscopy quantitatively evaluated Zn-solubilization, and the isolates were further studied for Zn-solubilization and plant growth in Solanum lycopersicum. The CDS7 and CDS27 isolates were the most significant Zn solubilizing strains. CDS7 exhibited increased ZnO solubility (32.1 mg/l) compared to CDS21 (23.7 mg/l). PGP trait quantitative results revealed that the CDS7 and CDS21 bacterial strains solubilized insoluble phosphate (287.2 and 217.7 μg/ml, respectively) and produced indole acetic acid (22.1 and 14.8 μg/ml, respectively). Based on 16S rRNA gene sequencing, CDS7 and CDS21 were identified as Pseudomonas kilonensis and Pseudomonas chlororaphis, and 16S rDNA sequences were submitted to the GenBank database. Furthermore, ZSB strains were administered to tomato seeds under a pot study. The treatments with CDS7 inoculant and a consortium of both isolates were reported with maximum plant development (stem length 63.16 and 59.89 cm, respectively) and zinc content (3.13 and 2.36 mg/100 g, respectively) in tomato fruit compared to the control. In conclusion, microorganisms isolated from cow dung with PGP activity can improve Zn bioavailability and plant growth sustainably. They can be used as biofertilizers in agricultural fields to improve plant growth and production.
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2023, 104, 2; 157-169
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Optimization of mycelial growth and cultivation of wild Ganoderma sinense
Autorzy:
Nguyen, Luyen Thi
Le, Ve Van
Nguyen, Bich Thuy Thi
Nguyen, Huyen Trang Thi
Tran, Anh Dong
Ngo, Nghien Xuan
Powiązania:
https://bibliotekanauki.pl/articles/16668411.pdf
Data publikacji:
2023
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
Ganoderma sinense
mycelial growth
spawn
lingzhi mushroom
Opis:
Ganoderma sinense, a well-known medicinal macrofungus of Basidiomycetes, is widely used in traditional medicine for promoting health and longevity in East Asia. The fruiting bodies of G. sinense contain polysaccharides, ergosterol, and coumarin, which have antitumor, antioxidant, and anticytopenia activities. Mushroom cultivation requires suitable conditions for the formation of fruiting bodies and yield. However, little is known about the optimal culture conditions for mycelial growth and cultivation of G. sinense. In this study, the successful cultivation of a G. sinense strain collected from the wild was reported. The optimal culture conditions were identified by examining one factor at a time. The results of this study revealed that the nutritional requirements for the optimal mycelial growth of G. sinense were fructose (15 g/l) as the carbon source and yeast extract (1 g/l) as the nitrogen source. The optimal pH and temperature for G. sinense were 7 and 25–30EC, respectively. The mycelia grew fastest in treatment II (69% rice grains + 30% sawdust + 1% calcium carbonate). G. sinense produced fruiting bodies under all tested conditions and showed the highest biological efficiency (2.95%) in treatment B (96% sawdust, 1% wheat bran, 1% lime). In summary, under optimal culture conditions, G. sinense strain GA21 showed satisfactory yield and a high potential for commercial cultivation.
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2023, 104, 1; 65-74
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
GrxS17, a multidomain glutaredoxin acting in meristems
Autorzy:
Riondet, C.
Bashandy, T.
Rey, P.
Gaymard, F.
Vignols, F.
Rouhier, N.
Reichheld, J.
Powiązania:
https://bibliotekanauki.pl/articles/80948.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
glutaredoxin
protein
light condition
growth condition
root meristem
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effects of different hormonal treatments on growth parameters and secondary metabolite production in organ culture of Hyssopus officinalis L.
Autorzy:
Shoja, H.M.
Shishavan, H.K.
Powiązania:
https://bibliotekanauki.pl/articles/2096354.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
growth parameters
Hyssopus officinalis
photosynthetic pigment
secondary metabolite
thidiazuron
Opis:
Hyssopus officinalis L. is a medicinal plant containing valuable phytochemicals and is used extensively in the pharmaceutical industry. As this plant naturally contains low levels of these metabolites, plant cell and tissue culture technologies are used to produce them in large volumes. The present study aimed first to evaluate the effects of different types of cytokinins, including benzyl adenine (BA), kinetin (KIN), and thidiazuron (TDZ), at 1 ppm concentration in the culture medium on growth parameters and production of secondary metabolites and photosynthetic pigments in organ culture of H. officinalis; in the second step, the study investigated the effect of different concentrations (0, 0.2, 1, 1.5, and 2 ppm) of the most effective type of cytokinin. The results showed that TDZ was the optimal type of growth regulator compared to BA and KIN as it showed a positive influence on the studied traits; furthermore, its highest concentration of 2 ppm in culture medium was determined to be the best one that enabled the highest production of secondary metabolites and photosynthetic pigments. We conclude that the presence of growth regulators can strongly influence the growth and development of plants in tissue culture conditions.
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2021, 102, 1; 33-41
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effects of thidiazuron on in vitro shoot regeneration of Morus alba
Autorzy:
Rezaei-Zafarghandi, M.S.
Rahmati-Joneidabad, M.
Powiązania:
https://bibliotekanauki.pl/articles/2096960.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
mulberry
cotyledon
plant growth regulator
organogenesis
in vitro-grown seedlings
Opis:
Mulberry (Morus alba L.) is an ornamental, medicinal, and fruit plant that belongs to the Moraceae family. One of the most important techniques used in plant biotechnology is tissue culture, which enables mass production of pathogen-free plants. Cotyledon has a high potential for shoot regeneration; however, to the best of our knowledge, there are no reports on plant regeneration from cotyledon explants of mulberry. Therefore, this study aimed to evaluate the potential of seedling-derived cotyledon segments to obtain shoot multiplication of mulberry. Various concentrations (0, 0.25, 0.5, 1, 2, 3, and 4 mg/l) of thidiazuron (TDZ) in combination with indole butyric acid (IBA) were used in a completely randomized design in three replications. The results showed that the highest percentage of regeneration frequency (96.67%) and the maximum number of shoots (4.43) were obtained on Murashige and Skoog (MS) medium supplemented with 0.25 mg/l TDZ and 0.025 mg/l IBA. In the rooting experiment, the maximum rooting percentage (83.33%) and the maximum number of roots per shoot (4.36) were obtained on MS medium containing 2 mg/l IBA. In vitro-raised plantlets were placed in pots and kept in room temperature for 30 days, and the plantlets showed more than 90% survival rate. On the basis of our results, the protocol described in this study has a high potential to be used in the micropropagation of this valuable plant.
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2020, 101, 1; 55-61
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of halotolerant plant growth-promoting rhizobacteria from Bougainvillea glabra on wheat and maize seedlings under NaCl stress
Autorzy:
Karnwal, A.
Powiązania:
https://bibliotekanauki.pl/articles/2096294.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
abiotic stress
salinity stress
seedling growth
rhizobacteria
staple food
Opis:
Wheat and maize are the main staple food crops that contribute to human food security. Their growth, however, is reduced under stresses such as salinity. The plant microbiome is associated with each plant tissue and develops a holobiont in association with the plant. Plants actively manage the configuration of their related bacterial population and its function. These microorganisms provide a broad range of benefits and advantages to the plants. The present study aimed to examine the growth improvement of wheat var. HD 2687 and maize var. PSCL-4642 under salinity at the seedling stage following inoculation of salt-tolerant plant growth-promoting rhizobacteria (PGPR) BoGl120 purified from Bougainvillea glabra. The seed germination potential with/without bacterial inoculation was examined at 50, 100, 150, and 200 mM NaCl concentrations for both crops. Compared to controls, at 50 mM NaCl concentration, the BoGl120 isolate provided the maximum radicle length in maize (32 mm) and in wheat (24.8 mm). At 100 mM NaCl concentration, however, the radicle length of wheat and maize seedlings was decreased. Inoculation of plants with the BoGl120 isolate enhanced the plumule length of seedlings at different NaCl concentrations as compared to controls. In comparison with controls, BoGl120 improved the plumule length of wheat to 32.6, 14.0, and 8 mm at 50, 100, and 150 mM NaCl concentrations, respectively. The results of the present study support the concept that PGPRs could help to increase the tolerance against saline stress in wheat and maize at the seedling stage.
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2020, 101, 4; 349-359
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of salt stress-tolerant bacterial endophytes from Bougainvillea glabra on the growth of Triticum aestivum L. var. HD 2687 and Zea mays var. PSCL-4642
Autorzy:
Karnwal, A.
Powiązania:
https://bibliotekanauki.pl/articles/2096990.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Bougainvillea glabra
NaCl
plant growth
seed germination
saline stress
Opis:
Wheat and corn crops contribute to the food security of humans by providing a nutrient-rich diet. However, their production in abiotic stress conditions such as salinity is limited. Endophytes exert a beneficial effect on plants through the decomposition of organic materials for smooth absorption, detoxification, and reduction of the effect of phytopathogenic microorganisms by increasing the immunity of host plants to resist phytopathogens and through nutrient deposition in plants responsible for reducing salt stress. The present study aimed to evaluate the NaCl tolerance efficiency of Triticum aestivum L. var. HD 2687 and Zea mays var. PSCL-4642 cultivars at the germination stage after inoculation with salt-tolerant bacterial endophyte BoGl21 isolated from Bougainvillea glabra. The seeds of both crops were tested for percentage of seed germination with/without bacteria at 50, 100, 150, and 200 mM NaCl concentrations. The BoGl21 isolate induced a significant increase in radicle length in corn (25.6 mm) as compared to that in wheat (10.3 mm) at 50 mM NaCl. However, at 100 mM NaCl, the radical length of wheat and corn seedlings was 5 mm and 8.8 mm, respectively. Inoculation of maize and wheat with the bacterial isolate significantly increased the plumule length of the germinated seeds as compared to that of controls. BoGl21 increased the plumule length of wheat as compared to that of the control seeds by 31.9, 11.7, and 4.8 mm at 50, 100, and 150 mM salinity stress, respectively. Inoculation of corn seeds with BoGl21 at the tested NaCl levels (50, 100, and 150 mM NaCl) increased the plumule length of the germinated seeds by 33.1, 22, 13.2, and 3.2 mm, respectively. The current research results support the hypothesis that bacterial endophytes could be beneficial to minimize the toxicity of saline stress on wheat and corn at the time of germination.
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2020, 101, 2; 89-99
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of TAT-signaling fusion system along with co-expression of GroEL/ES chaperones on secretory expression of somatropin
Autorzy:
Rabbani, M.
Ghasemi, R.
Bagherinejad, M.R.
Jahanian, A.
Powiązania:
https://bibliotekanauki.pl/articles/2096995.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
chaperone
GroEL/ES
human growth hormone
somatropin
TAT system
Opis:
Human growth hormone (somatropin) is one of the most widely used recombinant proteins that stimulates growth, cell reproduction, and cell regulation in humans. Synthetic production of this protein normally results in low yields and inclusion body formation. To overcome these difficulties, the production of somatropin along with two common signal peptides, namely TorA and SufI, in co-expression with a cytosolic chaperone, GroEL/ES, was evaluated in the present study. The target protein and the two signal sequences (TorA and SufI) were synthesized and cloned into an expression plasmid (pET-22) by using Nde l and Xho l endonucleases. The expression vector (pGro7) containing chaperone proteins (GroES/EL) and one of the expression vectors containing the signal sequence (and the target protein) were co-expressed in the BL21 DE3 expression host. The results showed that although some of the expressed proteins exit the cytoplasm and enter the periplasmic space, there is also an accumulation of proteins (probably as inclusion body) inside the cytoplasmic area. Western blot analysis showed that the inclusion of a signal sequence in the cassette containing the target protein could help to secrete the protein in the periplasmic space and culture media when compared with control groups. The result of these experiments show that the TAT secretion system promotes transportation of the target protein out of the cytoplasm. This secretory system completes folding of the protein structure and transfers the mature protein to the periplasmic space.
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2020, 101, 2; 101-108
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Influence of growth regulators and genotype on callus induction from cotyledonary explants of Camelina sativa (L.) Crantz
Autorzy:
Mikolajczyk, S.
Weigt, D.
Kurasiak-Popowska, D.
Tomkowiak, A.
Bazylewska, J.
Pluta, M.
Powiązania:
https://bibliotekanauki.pl/articles/951292.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
false flax
Camelina sativa
cotyledonary stage
callus induction
growth regulator
genotype
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Assessment of plant growth-promoting stress-tolerant endophytes screened from Bougainvillea glabra for the growth of Triticum aestivum L. and Zea mays
Autorzy:
Karnwal, A.
Powiązania:
https://bibliotekanauki.pl/articles/2096951.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
bacterial endophyte
dendrogram
molecular analysis
plant growth development
salinity tolerance
Opis:
Globally, more than 5.2 billion hectares of farming fields are damaged through erosion, salinity, and soil deterioration. Salt stress-tolerant bacteria have plant growth-promoting (PGP) characteristics that can be used to overcome environmental stresses. Isolation and screening of salt-tolerant endophytes from Bougainvillea glabra were achieved through surface sterilization of leaves, followed by their cultivation on 0.5% NaCl-supplemented media. The performance of isolates for indole acetic acid (IAA) production, phosphate solubilization, ACC deaminase activity, ammonia production, siderophore production, and stress tolerance was determined. In the present study, 5 morphologically distinct salt-tolerant endophytic bacteria were cultured. Out of 5 isolates, 60% salt-tolerant endophytes showed phosphate solubilization, 100% IAA production, 40% ACC-deaminase activity, 100% siderophore production, 40% ammonia production and 60% HCN production. Dendrogram generated based on stress tolerance showed three clusters: clusters 1 and 2 containing two isolates and cluster 3 containing 1 isolate. Based on the highest PGP activities, BoGl17 and BoGl21 isolates were chosen and further tested for wheat and corn growth promotion. The isolates increased wheat shoot and root dry weight by 40% and 80%, and by 69% and 126% for BoGl17 and BoGl21, respectively. The bacterial isolates identified as Pseudomonas reidholzensis BoGl17 had 95.59% identity with Pseudomonas reidholzensis strain ID3, while the isolate Bacillus aerius BoGl21 had 90.96% identity with Bacillus aerius strain 24K based on phenotypic features and 16S rRNA gene sequencing results. Both isolates significantly improved plant growth as compared to uninoculated plants.
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2020, 101, 1; 63-73
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The effect of temperature on growth of Thalassiosira pseudonana in vitro system
Autorzy:
Bojko, M.
Wanda, P.
Latowski, D.
Kuczynska, P.
Olchawa-Pajor, M.
Strzalka, K.
Powiązania:
https://bibliotekanauki.pl/articles/80198.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
diatom
Thalassiosira pseudonana
growth
gene sequence
temperature
in vitro
photosynthetic pigment
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Exploration of phytate-mineralizing bacteria with multifarious plant growth-promoting traits
Autorzy:
Kaur, Rajinder
Kaur, Sukhminderjit
Powiązania:
https://bibliotekanauki.pl/articles/16648096.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
phytate-mineralizing bacteria
phosphate solubilization
plant growth-promoting potential
biofertilizers
bioformulations
phosphorus
Opis:
Phytate-mineralizing bacteria (PMB) with plant growth-promoting activity can be considered as a potential biofertilizer for plant nutrition. PMB catalyzes the conversion of insoluble sugar phosphates, inositols, nucleic acids, phospholipids, nucleotides, phytate, and phytin into soluble forms that can be assimilated by plants. The present study aimed to isolate potential PMB from rhizospheric soils and to study their plant growth-promoting potential for the possible development of a potential phosphobacterium biofertilizer. For this purpose, 34 PMB isolates were isolated that showed potent phytate-mineralizing potential. These isolates were tested for their potential to solubilize tricalcium phosphate (TCP) and for various other plant growth-promoting activities. Significant differences were found among the isolates with regard to phytate mineralization and other plant growth-promoting characteristics. The bacterial isolates biochemically identified as Bacillus, Paenibacillus, Arthrobacter, and Burkholderia exhibited high/medium P solubilization, medium/high phytohormone production, and medium/low siderophore and ammonia production. Among all these isolates, isolate A14 (Burkholderia cenocepacia strain FDAARGOS_7) was the promising isolate with high TCP solubilization, medium phytate mineralization, high enzyme production, medium/high phytohormone production, and medium ammonia production. This strain also showed nitrogen fixation activity, zinc solubilizing potential, potassium solubilization, ACC deaminase production, and catalase production. Hence, it can be concluded that B. cenocepacia can be the potential candidate for biofertilizer development. Future studies are planned for exploring the role of PMB in biofertilizer formulations.
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2022, 103, 2; 99-112
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Changes in melatonin content in conditioned corn (Zea mays L.) and cucumber (Cucumis sativus L.) seeds
Autorzy:
Rakowiec, M.
Parczewska, A.
Szewczyk, R.
Kolodziejczyk, I.
Posmyk, M.M.
Powiązania:
https://bibliotekanauki.pl/articles/80461.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
exogenous melatonin
corn
Zea mays
cucumber
Cucumis sativus
seed
germination
seedling growth
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The use of a 2-aminoethylphosphonate by the mesophilic Penicillium commune strain
Autorzy:
Stosiek, N.
Klimek-Ochab, M.
Powiązania:
https://bibliotekanauki.pl/articles/80632.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
fungal strain
Penicillium commune
growth condition
2-aminoethylphosphonate
biodegradation
high performance liquid chromatography
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2018, 99, 4
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A preliminary study on some Chlorella spp. for biodiesel production
Autorzy:
Mohammady, N.G.-E.
El-Sayed, H.S.
El-Kassas, H.Y.
Elsherbiny, B.A.
Powiązania:
https://bibliotekanauki.pl/articles/80100.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
biodiesel production
Chlorella
fatty acid
growth
lipid
oil
esterification
fatty acid methyl ester
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2017, 98, 4
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
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