Autor: Ag. - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Influence of short-term ammonium supply on ROS metabolism in Arabidopsis
Autorzy:: Podgorska, A.
Rasmusson, A.G.
Szal, B.
Powiązania:: https://bibliotekanauki.pl/articles/80357.pdf
Data publikacji:: 2013
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: conference
ammonium
reactive oxygen species
Arabidopsis
leaf cell
oxidative stress
homeostasis
antioxidative enzyme
Źródło:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
0860-7796
Pojawia się w:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Seed germination dynamics of some woody legumes: implication for restoration of arid zones ecosystems
Autorzy:: Buba, T.
Ezra, A.G.
Bako, S.P.
Sabo, M.U.
Powiązania:: https://bibliotekanauki.pl/articles/28825291.pdf
Data publikacji:: 2023
Wydawca:: Polska Akademia Nauk. Czasopisma i Monografie PAN
Źródło:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2023, 104, 4; 381-402
0860-7796
Pojawia się w:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Improved transformation of Agrobacterium assisted by silver nanoparticle
Autorzy:: Benny, Amala
Alex, Swapny
Soni, K.B.
Anith, K.N.
Kiran, A.G.
Viji, M.M.
Powiązania:: https://bibliotekanauki.pl/articles/16648111.pdf
Data publikacji:: 2022
Wydawca:: Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:: silver nanoparticles
Agrobacterium transformation
calcium chloride
competent cells
freeze thaw
transformation efficiency
Opis:: In transgenic plant development, the low transformation efficiency of Agrobacterium with exogenous DNA is the major constraint, and hence, methods to improve its transformation efficiency are needed. Recently, nanoparticlemediated gene transfer has evolved as a key transformational tool in genetic transformation. Since silver nanoparticles (AgNPs) can induce pores on the cell membrane, their efficacy in the improvement of conventional calcium chloride freeze-thaw technique of transformation of Agrobacterium was explored in this study. Agrobacterium cells in the exponential growth phase were exposed to different concentrations of AgNPs (0.01, 1, 5, 10, and 20 mg/l), and the half-maximal effective concentration (EC50) was determined via Probit analysis using the SPSS software. Transformation efficiency of AgNPs alone and in combination with calcium chloride was compared with that of the conventional calcium chloride freeze-thaw technique. AgNPs at a concentration of 0.01 mg/l in combination with calcium chloride (20 mM) showed a ten fold increase in the transformation efficiency (3.33 log CFU (colony-forming unit/microgram of DNA) of Agrobacterium tumefaciens strain EHA 105 with plasmid vector pART27 compared with the conventional technique (2.31 log CFU/μg of DNA). This study indicates that AgNPs of size 100 nm can eliminate the freeze-thaw stage in the conventional Agrobacterium transformation technique, with a 44% improvement in efficiency. The use of AgNPs (0.01 mg/l) along with 20 mM calcium chloride was found to be an economically viable method to improve the transformation of Agrobacterium with exogenous plasmid DNA.
Źródło:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2022, 103, 3; 311-317
0860-7796
Pojawia się w:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:: Biblioteka Nauki

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