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Wyświetlanie 1-15 z 24
Tytuł:
In vivo confocal microscopy and in vitro culture techniques as tools for evaluation of severe Acanthamoeba keratitis incidents
Autorzy:
Padzik, Monika
Szaflik, Jacek P.
Baltaza, Wanda
Perkowski, Konrad
Dybycz, Monika
Chomicz, Lidia
Powiązania:
https://bibliotekanauki.pl/articles/972158.pdf
Data publikacji:
2017
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
Acanthamoeba keratitis
in vitro cultivation
in vivo confocal microscopy
Opis:
Amphizoic amoebae belonging to the genus Acanthamoeba are known as etiological agents of sightthreatening Acanthamoeba keratitis. The leading risk factor for the development of this serious human disease is contact lens wearing which popularity increases worldwide, also in Poland. The disease with active epithelial inflammations, corneal ulcers, including loss of the visual acuity is a serious medical problem as an emerging threat for the public health related to improper contact lens hygiene. The treatment of the amoebic keratitis is difficult, often unsuccessful due to delayed proper diagnosis. The clinical picture of the disease, often with severe course is nonspecific, similar to that occurring in viral, fungal or bacterial keratitis, thus clinical symptoms alone are not sufficient to identify the causative agent of the amoebic infection. Early diagnosis is decisive for the suitable therapeutic management and the treatment efficacy. In our studies, several complicated, difficult to treat Acanthamoeba keratitis incidences pertaining Polish patients using contact lenses have been retrospectively analyzed in terms of the usefulness of non-invasive methods of in vivo confocal microscopy and in vitro culture techniques applied for diagnosis. Hyper-reflective double-walled spherical Acanthamoeba cysts, with a more reflective outer wall were detected in the epithelium and anterior layers of the corneal stroma. In vivo confocal microscopy, if available, may be a valuable, sensitive tool for diagnosis in late identified severe infections mainly with strong viability strains, however confoscan may offer limited value at lowintensity amoebic infections. The microscopic visualization of amoebae in slides prepared directly from corneal scraping and laboratory examinations of specimens from in vitro cultivated corneal isolates allow to confirm or verify results of in vivo examinations, furthermore to identify directly the pathogens and to clarify previous misdiagnoses.
Źródło:
Annals of Parasitology; 2017, 63, 4; 341-346
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Amoebicidal or amoebostatic influence of disinfectants used in health facilities and laboratories on corneal strains of Acanthamoeba
Autorzy:
Baltaza, Wanda
Padzik, Marcin
Szaflik, Jacek P.
Dybicz, Monika
Hendiger, Edyta
Chomicz, Lidia
Powiązania:
https://bibliotekanauki.pl/articles/972267.pdf
Data publikacji:
2017
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
in vitro dynamics
influence of disinfectants
Acanthamoeba strains
Opis:
Different Acanthamoeba species are amphizoic organisms distributed in wide range of habitats in natural and man-made environments; they are also detected on surfaces of equipment and accessories in health facilities. Some strains of the amoebae are causative agents of the vision-threatening human disease Acanthamoeba keratitis, mainly reported in contact lens wearers. An exceptional high resistance of Acanthamoeba trophozoites and particularly cysts to chemicals, disinfectants and drugs is believed as influencing difficulty resulting in unsuccessful therapeutic management. As Acanthamoeba keratitis is the serious medical problem worldwide, different chemicals with possible activity against environmental and clinical Acanthamoeba strains are tested. In our study, selected disinfectants used in health care settings and laboratories were tested and their efficacy against the corneal strains Acanthamoeba castellanii and A. polyphaga , and environmental A. castellanii Neff strain was assessed. Comparative assessment of results of the assays show that, apart from amoebistatic effects, the disinfectants indicated expected cysticidal efficacy.
Źródło:
Annals of Parasitology; 2017, 63, 3; 162-172
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The activity in vitro of itraconazole and ketoconazole against pathogenic fungi
Autorzy:
Kurnatowska, A.
Horwatt-Bozyczko, E.
Powiązania:
https://bibliotekanauki.pl/articles/839467.pdf
Data publikacji:
1998
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
activity
pathogenic fungi
fungi
ketoconazole
in vitro
itraconazole
Źródło:
Annals of Parasitology; 1998, 44, 3
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Badania in vitro lekoodpornosci szczepow grzybow z rodzaju Candida
Autorzy:
Banach-Piatkowska, W
Kotlowski, A.
Humanowska, J.
Mayer, L.
Kowalczyk, D.
Powiązania:
https://bibliotekanauki.pl/articles/840174.pdf
Data publikacji:
2001
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
lekoopornosc
grzyby
szczepy chorobotworcze
Candida
leki przeciwgrzybicze
badania in vitro
Opis:
The purpose of this study was to determine the in vitro susceptibility to 6 antifungal agents of 143 strains of Candida species isolated from 545 patients with suspicion of fungal infection. Test ATB Fungus (bioMerieux) was used. Among all Candida isolates 91.6% were susceptible to tested polyenes and 32.9% to all azoles. Less susceptible to polyenes isolates were noted in low percentage (0.7 to 4.2%) and to azoles more frequcntly (32.9 to 41.2%). Candida isolates resistant to antifungal agents tested were dependent from the kind of clinical samples and species of fungi. Monitoring of the susceptibility to antifungal agents by ATB commercial test seems to be uscful tool for therapeutic purposes.
Źródło:
Annals of Parasitology; 2001, 47, 4; 811-816
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Influence of interferon-gamma on the in vitro growth of Toxoplasma gondii
Autorzy:
Konopka, E.
Dzbenski, T.
Powiązania:
https://bibliotekanauki.pl/articles/841123.pdf
Data publikacji:
1998
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
parasite
mouse
mice
macrophage
Toxoplasma gondii
interferon gamma
growth
in vitro
Źródło:
Annals of Parasitology; 1998, 44, 3
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Influence of Anisakis simplex stage III larvae upon the activity of proteases under in vitro conditions
Autorzy:
Dziekonska-Rynko, J
Rokicki, J.
Jablonowski, Z.
Powiązania:
https://bibliotekanauki.pl/articles/837165.pdf
Data publikacji:
2002
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
protease activity
stage
papain
pepsin
trypsin
larva
Anisakis simplex
in vitro
Opis:
The larvae of Anisakis simplex had the largest influence upon decreasing the activity of porcine pepsin. The activity of that enzyme in tests, where the larvae were present during the entire period of incubation, was lower than in the controls. No similar trends were observed in case of the solutions with bovine and porcine trypsin. The activity of those enzymes in the solutions containing the larvae was higher than in the controls. Only the activity of porcine trypsin after 10 h of incubation was slightly lower in the experimental sample than in the control, however, during the later hours the dynamics of the activity decrease of that enzyme in the controls was higher than in the experimental samples. The recorded activity of papain in the samples containing the larvae was higher than that in the controls during the entire time of the experiment.
Źródło:
Annals of Parasitology; 2002, 48, 2
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Towards control of parasitic nematodes in domestic animals by microfungi
Autorzy:
Nansen, P.
Powiązania:
https://bibliotekanauki.pl/articles/837554.pdf
Data publikacji:
1998
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
screening
parasite
in vivo
animal
nematode
fungi
in vitro
Duddingtonia flagrans
domestic animal
Źródło:
Annals of Parasitology; 1998, 44, 3
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
In vitro antifungal susceptibility testing of Scopulariopsis brevicaulis strains using agar diffusion method
Ocena in vitro lekowrażliwości szczepów Scopulariopsis brevicaulis metodą dyfuzji w agarze
Autorzy:
Skora, M.
Macura, A.B.
Powiązania:
https://bibliotekanauki.pl/articles/841599.pdf
Data publikacji:
2011
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
in vitro
antifungal susceptibility
testing
Scopulariopsis brevicaulis
agar diffusion test
human infection
Opis:
The genus Scopulariopsis is a common soil saprotroph and has been isolated from air, organic waste and also from plant, animal and human tissues. Scopulariopsis has mainly been associated in humans with superficial mycoses, but it has also been described as the cause of subcutaneous and invasive infections. The most common aetiological agent of infections in humans is Scopulariopsis brevicaulis. This species has been reported to be resistant in vitro to broad-spectrum antifungal agents available today. The aim of the study was to establish in vitro antifungal susceptibility of 35 S. brevicaulis strains against amphotericin B (AMB), flucytosine (FC), caspofungin (CAS), terbinafine (TER), ciclopirox (CIC), voriconazole (VOR), clotrimazole (CTR), miconazole (MCZ), econazole (ECO), ketoconazole (KET), itraconazole (ITR), and fluconazole (FLU). Antifungal susceptibility tests were evaluated by an agar diffusion method (Neo-Sensitabs, Rosco, Denmark). AMB, FC, CAS, ITR and FLU showed no antifungal activity against S. brevicaulis. TER, CIC, CTR, KET, VOR, ECO, and MCZ revealed inhibitory activity for S. brevicaulis, but it varied for each of the drugs. The best antifungal effect was observed for TER and CIC. All isolates had large inhibition zones for TER and CIC. CTR was also inhibitory for all tested S. brevicaulis isolates, but the diameters of inhibition zones were smaller than for TER and CIC. Nearly 89% isolates showed inhibition zones for KET and the mean diameter of the inhibition zone was comparable to CTR. The least antifungal activity exhibited VOR, ECO and MCZ. Because of the multiresistance of S. brevicaulis, infections due to this species may not respond to particular antifungal treatment and other therapeutic approaches should be considered e.g., combined therapy and/or surgery.
Źródło:
Annals of Parasitology; 2011, 57, 2
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wzrost populacji Balantidium coli w warunkach in vivo i in vitro
Autorzy:
Skotarczak, B
Zielinski, R.
Powiązania:
https://bibliotekanauki.pl/articles/839984.pdf
Data publikacji:
1997
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
Balantidium coli
hodowla in vivo
pasozyty zwierzat
liczebnosc populacji
pierwotniaki
parazytologia
hodowla in vitro
Opis:
Trophozoites of Balantidium coli were isolated from the pig's caecum and cultivated in vitro. Pigs were divided into two groups: one with the acute balantidiosis and the second - with the asymptomatic balantidiosis. In the first case the biotic potential of protozoans turned out to be higher (the most intensive divisions occurred 24 hours after the second passage). Protozoans isolated from pigs with the asymptomatic balantidiosis had lower biotic potential (the most intensive divisions occurred 24 hours after the third passage). This fact indicates the differentiation of the investigated populations of B. coli.
Źródło:
Annals of Parasitology; 1997, 43, 2; 171-178
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
In vitro susceptibility of Giardia isolates of human and animal origin to natamycin
Autorzy:
Majewska, A.C.
Powiązania:
https://bibliotekanauki.pl/articles/837538.pdf
Data publikacji:
1998
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
metronidazole
man
Giardia
drug
giardiasis
animal
natamycin
Protozoa
ornidazole
in vitro
parasitosis
Źródło:
Annals of Parasitology; 1998, 44, 3
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The impact of in vitro selection with closantel on development of Fasciola hepatica
Autorzy:
Klockiewicz, M.
Pisarek, A.
Wedrychowicz, H.
Powiązania:
https://bibliotekanauki.pl/articles/836445.pdf
Data publikacji:
1998
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
parasite
selection
Oligocene water
life cycle
Fasciola hepatica
in vitro
Galba truncatula
host
closantel
Źródło:
Annals of Parasitology; 1998, 44, 3
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Badania in vitro wplywu albendazolu na kinetyke reakcji katalizowanej przez cytozolowa transferaze glutationowa [GST] z watroby szczura
Effect in vitro of albendazole on the kinetics of cytosolic glutathione transferase from the rat liver
Autorzy:
Wojtkowiak, A
Boczon, K.
Wandurska-Nowak, E.
Powiązania:
https://bibliotekanauki.pl/articles/836932.pdf
Data publikacji:
2007
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
szczury
katalizatory
kinetyka reakcji enzymatycznych
albendazol
transferaza S-glutationowa
watroba
transferazy glutationowe
badania in vitro
Źródło:
Annals of Parasitology; 2007, 53, 2; 97-102
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Ocena przydatnosci testow kolorymetrycznych MTT i XTT do badan prowadzonych in vitro na tachyzoitach Toxoplasma gondii
The utility of MTT and XTT colorimetric test in the studies conducted in vitro with Toxoplasma gondii tachyzoites
Autorzy:
Dzitko, K
Dudzinska, D.
Grzybowski, M.
Dlugonska, H.
Powiązania:
https://bibliotekanauki.pl/articles/838636.pdf
Data publikacji:
2010
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
Toxoplasma gondii
tachyzoity
badania in vitro
metody badan
kolorymetria
test MTT
test XTT
ocena przydatnosci
Źródło:
Annals of Parasitology; 2010, 56, 2; 145-152
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Ocena in vitro grzybow z rodzaju Aspergillus i Penicillium na nowe leki przeciwgrzybicze
Autorzy:
Macura, A B
Pawlik, B.
Szczepko, I.
Powiązania:
https://bibliotekanauki.pl/articles/841467.pdf
Data publikacji:
2000
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
plesnie
Penicillium
dzialanie lekow
Aspergillus
leki przeciwgrzybicze
parazytologia
ocena
dzialanie przeciwgrzybicze
badania in vitro
grzybice
Opis:
Fifty four mould strains (32 Aspergillus and 22 Penicillium) isolated from clinical materials were tested using dilution method. Two test media were used: Yeast Nitrogen Base (YNB) and Czapek Dox (CD). The following drugs were tested: amorolfine, cyclopirox, itraconazole, and terbinafine. In the dilution method, the drugs were tested at four contrentrations: 0.1 mg/l, 1 mg/l, 10 mg/l, and 100 mg/l. No matter which medium and/or drug was used, terbinafine turned out to be most effective. The drug at a conceatration of 1 mg/l inhibited 88.9% of the strains in the CD medium. The MIC values in the YNB medium varied from 0,1 mg/l through 100 mg/1, however, 50% of the strains were inhibited at 1 mg/l or less. Itraconazole revealed a fairly good in vitro antifungal action, particularly in the CD medium: 77.8% of the strains were inhibited at 10 mg/l or less. Aspergillus fumigatus and Aspergillus flavus were most susceptible to itraconazole. The MIC values of cyclopirox amounted to 100 mg/l for all of the strains in the CD medium but not in the YNB where they varied from 1 mg/l to 100 mg/l. Amorolfine was the least effective drug. Most of the strains were inhibited at a concentration of at least 100 mg/l. The findings give evidence that the susceptibility of Aspergillus and Penicillium to the particular drugs is different, and that the results are influenced by the test medium.
Źródło:
Annals of Parasitology; 2000, 46, 1; 157-162
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 24

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