Informacja

Drogi użytkowniku, aplikacja do prawidłowego działania wymaga obsługi JavaScript. Proszę włącz obsługę JavaScript w Twojej przeglądarce.

English (EN)·Polski (PL)·Yкраїнський (UK)
Przejdź do strony domowej biblioteki Centralna Biblioteka Wojskowa Link otwiera się w nowym oknie Logo biblioteki Prolib Integro - strona głównaCentralna Biblioteka Wojskowa

Wyszukujesz frazę "polymerase chain reaction" wg kryterium: Temat

  Lista filtrów
Wyrównaj
    Wyświetlanie 1-15 z 15
    Tytuł:
    Hantavirus RNA not detected in Ixodes ricinus ticks
    Autorzy:
    Wojcik-Fatla, A.
    Zajac, V.
    Knap, J.P.
    Dutkiewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/49890.pdf
    Data publikacji:
    2011
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    hantavirus
    RNA
    Ixodes ricinus
    tick
    epidemiology
    polymerase chain reaction
    Polska
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2011, 18, 2
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Virulence and antibiotic resistance of Escherichia coli isolated from rooks
    Autorzy:
    Kmet, V.
    Drugdova, Z.
    Kmetova, M.
    Stanko, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/51141.pdf
    Data publikacji:
    2013
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    virulence
    antibiotic resistance
    Escherichia coli
    isolation
    rook
    polymerase chain reaction
    DNA microarray
    Opis:
    With regard to antibiotic resistance studies in various model animals in the urban environment, the presented study focused on the rook, many behavioural and ecological aspects of which are important from an epidemiological point of view. A total of 130 Escherichia coli strains isolated from rook faeces during a two-year period (2011–2012) were investigated for antibiotic resistance and virulence. Resistance to ampicillin (60%) and streptomycin (40%) were the most frequent, followed by resistance to fluoroquinolones (ciprofloxacin-22% and enrofloxacin-24%), tetracycline (18%), cotrimoxazol (17%) and florfenicol (14%). Ceftiofur resistance occured in 10.7 % of strains and cefquinom resistance in 1.5 % of strains. Twenty-five E.coli strains with a higher level of MICs of cephalosporins (over 2mg/L of ceftazidime and ceftriaxon) and fluoroquinolones were selected for detection of betalactamase genes (CTX-M, CMY), plasmid-mediated quinolone resistance qnrS, integrase 1, and for APEC (avian pathogenic E.coli) virulence factors (iutA, cvaC, iss, tsh, ibeA, papC, kpsII). Genes of CTX-M1, CMY-2, integrase 1, papC, cvaC, iutA were detected in one strain of E.coli, and qnrS, integrase 1, iss, cvaC, tsh were detected in another E.coli. DNA microarray revealed the absence of verotoxin and enterotoxin genes and pathogenicity islands. The results show that rooks can serve as a reservoir of antibiotic-resistant E. coli with avian pathogenic virulence factors for the human population, and potentially transmit such E.coli over long distances.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2013, 20, 2
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Detectability of tick-borne agents DNA in the blood of dogs, undergoing treatment for borreliosis
    Autorzy:
    Wodecka, B
    Rymaszewska, A.
    Sawczuk, M.
    Skotarczak, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/50622.pdf
    Data publikacji:
    2009
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    dog
    tick-borne agent
    polymerase chain reaction
    RFLP method
    borreliosis
    anaplasmosis
    diagnostics
    blood
    treatment
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2009, 16, 1; 9-14
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    A small-scale survey of hantavirus in mammals from eastern Poland
    Autorzy:
    Wojcik-Fatla, A.
    Zajac, V.
    Knap, J.P.
    Sroka, J.
    Cisak, E.
    Sawczyn, A.
    Dutkiewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/51577.pdf
    Data publikacji:
    2013
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    hantavirus
    Bunyaviridae
    mammal
    small mammal
    Microtus agrestis
    Myodes glareolus
    Sorex araneus
    epidemiology
    polymerase chain reaction
    flood
    Polska
    Opis:
    Samples of 30 dead small mammals each were collected on area ‘A’ located in eastern Poland which is exposed to flooding by the Vistula river, and on the area ‘B’, also located in eastern Poland but not exposed to flooding. Kidneys and livers of the mammals were examined by the PCR and nested PCR methods for the presence of hantavirus RNA. Out of 7 species of small mammals examined, the presence of hantaviruses was detected in 4 of them. Hantavirus prevalence was low in Apodemus agrarius (2.6%), the most numerous mammal species, whereas in the remaining 3 positive species (Microtus agrestis, Myodes glareolus, Sorex araneus) this was 12.5–100%. The presence of hantaviruses was detected only in the animals found on area ‘A’ exposed to flooding, and their prevalence was statistically greater compared to area ‘B’ not exposed to flooding (16.7% vs. 0%, p=0.0345). The overall positivity of the examined small mammals population from the areas ‘A’ and ‘B’ was 8.3%. The sequence analysis of the samples positive for hantavirus proved that the amplified products showed 77–86% homology with the L segment sequence of hantavirus Fusong-Mf-731 isolated from Microtus fortis in China. The presented study is the first to demonstrate the occurrence of hantavirus infection in small mammals from eastern Poland, and the first to demonstrate the significant relationship between flooding and the prevalence of hantaviruses in small mammals.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2013, 20, 2
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Molecular and serological diagnosis of Borrellia burgdorferi infection among patients with diagnosed erythema migrans
    Autorzy:
    Kondrusik, M
    Grygorczuk, S.
    Skotarczak, B.
    Wodecka, B.
    Rymaszewska, A.
    Pancewicz, S.
    Zajkowska, J.
    Swierzbinska, R.
    Hermanowska-Szpakowicz, T.
    Powiązania:
    https://bibliotekanauki.pl/articles/51856.pdf
    Data publikacji:
    2007
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    human disease
    erythema migrans phase
    patient
    diagnosis
    ELISA test
    polymerase chain reaction
    serological diagnosis
    Borrelia burgdorferi
    borreliosis
    Opis:
    The aim of the study was to assess the frequency of Borrelia burgdorferi DNA detection in the blood and urine of patients diagnosed with erythema migrans, and compare the results of PCR-based methods with ELISA methodology. The latter was used to detect serum antibodies against Borrelia burgdorferi of the IgM and IgG classes, before and after antibiotic therapy. The study included 86 patients hospitalized in the Department of Infectious Diseases and Neuroinfections in the Medical Academy in Białystok, diagnosed with the erythema migrans phase of Lyme borreliosis. Examinations were carried out twice: the fi rst at the moment of diagnosis (Trial 1), the second after 4 weeks of antibiotic therapy. The study showed that antibiotic therapy in the early phase of borreliosis does not decrease the sensitivity of PCR and that after 4 weeks of therapy (Trial 2), spirochete DNA is still detectable in most patients (45/86). There was no correlation between detectability of spirochete DNA and the presence of antibodies against B. burgdorferi s.l. (assessed by ELISA) during the course of erythema migrans. The largest percentage of positive results in the detection of B. burgdorferi s.l. DNA was observed in patients who simultaneously possessed IgM and IgG antibodies against B. burgdorferi, while the lowest percentage of PCR positive results was among patients with only IgM antibodies.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2007, 14, 2
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Application of a duplex-PCR for detection of cows' milk in goats' milk
    Autorzy:
    Kotowicz, M
    Adamczyk, E.
    Bania, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/51721.pdf
    Data publikacji:
    2007
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    cow milk
    human disease
    goat milk
    food
    allergy
    polymerase chain reaction
    species identification
    milk-derived product
    detection
    milk
    Opis:
    A duplex-PCR method, with 2 pairs of primers recognizing sequences of mitochondrial D-loop region, was developed to identify cows’ milk in the milk of goats. The PCR was shown to be specifi c and sensitive, enabling the detection of less than 1% of cows’ milk added to the milk of goats. Simultaneous use of a primer pair for goats’ and cows’ mitochondrial DNA fragment prevented false negative results. The method was applied to track the presence of cow DNA in goat milk available on the Polish market. A total of 54 milk samples from 3 Polish (34) and one foreign producer (20) were examined. In 33 samples, cow DNA was detected, while 21 samples, including all of the 20 samples from foreign producers, produced the goat-specific product only.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2007, 14, 2
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Isolation, cultivation and identification of Borrelia burgdorferi genospecies from Ixodes ricinus from the city of Brno, Czech Republic
    Autorzy:
    Pejchalova, K
    Zakovska, A.
    Mejzlikova, M.
    Halouzka, J.
    Dendis, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/49816.pdf
    Data publikacji:
    2007
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    cultivation
    infection
    Brno
    infection risk
    Lyme borreliosis
    Czech Republic
    isolation
    polymerase chain reaction
    Borrelia burgdorferi
    Ixodes ricinus
    tick
    identification
    Opis:
    A total of 305 ticks (21 larvae, 243 nymphs, 19 females and 22 males) were collected by fl agging of vegetation in suburban woods of Pisárky Park (city of Brno) from July to October 2002. The midgut of each tick was dissected out and transferred individually into BSK-H medium. After cultivation, all specimens were examined by dark-fi eld microscopy (DFM) for the presence of borreliae. Out of 305 tick samples, 45 were (14.8%) DFM positive. The following polymerase chain reaction (PCR) then revealed 37 (12.1%) samples positive for the presence of Borrelia burgdorferi sensu lato DNA. All 37 samples were further analysed by restriction fragment length polymorphism (RFLP) method. PCR-RFLP analysis revealed 14 strains of B. afzelii (37.8%), 15 strains of B. garinii (40.5%) and 2 strains of B. burgdorferi sensu stricto (5.4%). Four samples (10.8%) showed a mixed population of these genospecies. Two samples produced atypical RFLP pattern which were detected by sequence analysis as B. valaisiana (5.4%). Isolation attempts resulted in 21 spirochaetal strains (including two stains of B. valaisiana). The results show the diversity of B. burgdorferi s.l. in tick population and refer the risk of infection by pathogenic borreliae in Brno.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2007, 14, 1
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The occurrence of Toxoplasma gondii and Borrelia burgdorferi sensu lato in Ixodes ricinus ticks from East Poland with the use of PCR
    Autorzy:
    Sroka, J
    Szymanska, J.
    Wojcik-Fatla, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/51137.pdf
    Data publikacji:
    2009
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    Polska
    tick
    Ixodes ricinus
    Toxoplasma gondii
    Borrelia burgdorferi
    occurrence
    polymerase chain reaction
    genotyping
    B1 gene
    human population
    infection
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2009, 16, 2; 313-319
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Preliminary study on the occurrence of Toxoplasma gondii in Ixodes ricinus ticks from north-western Poland with the use of PCR
    Autorzy:
    Sroka, J
    Wojcik-Fatla, A.
    Zwolinski, J.
    Zajac, V.
    Sawczuk, M.
    Dutkiewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/51615.pdf
    Data publikacji:
    2008
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    polymerase chain reaction
    parasite
    genotyping
    Polska
    tick
    toxoplasmosis
    Ixodes ricinus
    B1 gene
    zoonotic disease
    occurrence
    Toxoplasma gondii
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2008, 15, 2
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    A 56-year-old man with RT-PCR negative nasopharyngeal swabs with Coronavirus Disease 2019 (COVID-19) Pneumonia
    Autorzy:
    Dworzańska, A.
    Tudrujek-Zdunek, M.
    Mosiewicz, J.
    Panasiuk, L.
    Tomasiewicz, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/2085530.pdf
    Data publikacji:
    2020
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    RT-PCR
    pneumonia
    Covid-19
    Coronavirus Disease 2019
    chest computed tomography
    real-time-reverse transcription-polymerase chain-reaction
    Opis:
    Introduction. Diagnostic procedure in Coronavirus Disease 2019 (COVID-19) is based mainly on performing real-time-reverse transcription-polymerase chain-reaction (RT-PCR), which has been accepted as the gold standard method. In some cases, such as mutations of the SARS-CoV-2 genome, variable viral load kinetics or laboratory errors, it can be false-negative. Case report. The case is presented of a 56-year-old man with respiratory tract symptoms, with twice negative results of real-time-reverse transcription-polymerase chain-reaction of nasopharyngeal swabs and positive chest computed tomography, with typical findings for COVID-19 pneumonia. Conclusions. Patients with negative RT-PCR results, but with positive computed tomography findings characteristic for COVID-19, should be treated as well as those infected.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2020, 27, 2; 317-318
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    What is percentage of pathogenic borreliae in spirochaetal findings of mosquito larvae?
    Autorzy:
    Nejedla, P
    Norek, A.
    Vostal, K.
    Zakovska, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/51950.pdf
    Data publikacji:
    2009
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    mosquito
    larva
    Culex
    spirochaete
    polymerase chain reaction
    Borrelia burgdorferi
    Culex pipiens
    human disease
    tick
    Ixodes ricinus
    midgut
    Czech Republic
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2009, 16, 2; 273-276
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Serological and molecular survey of Anaplasma phagocytophilum in Italian hunting dogs
    Autorzy:
    Ebani, V.V.
    Bertelloni, F.
    Turchi, B.
    Cerri, D.
    Powiązania:
    https://bibliotekanauki.pl/articles/50930.pdf
    Data publikacji:
    2013
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    serological survey
    molecular survey
    Anaplasma phagocytophilum
    hunting dog
    dog
    polymerase chain reaction
    indirect immunofluorescence antibody test
    tick
    zoonotic pathogen
    Italy
    prevalence
    Opis:
    The presence of Anaplasma phagocytophilum, a tick-transmitted zoonotic pathogen, has been reported in Italy in humans and several animal species, including dogs, but data concerning its prevalence in the Italian canine population are lacking. The aim of this study was to determine the prevalence of A. phagocytophilum infection in hunting dogs which live in geographic areas of central Italy where the infection had been previously detected in wild animals. Sera from 215 hunting dogs were tested by indirect immunofluorescence antibody test (IFAT) to detect antibodies to A. phagocytophilum. Buffy coat samples from the same 215 animals were submitted to a polymerase chain reaction (PCR) assay specific for the A. phagocytophilum 16S rRNA gene. Thirty-two (14.8%) dogs proved to be seropositive with antibody titres from 1:40–1:2,560. The highest seroprevalence was observed in dogs 6–10-year-old. Two seropositive (0.9%) animals were also PCR positive. Sequencing of PCR products revealed gene sequences of A. phagocytophilum in both cases. These results confirm the presence of A. phagocytophilum in the Italian areas studied; thus, the zoonotic potential of this agent should be considered particularly for people, as hunters, at risk of exposure to tick bites.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2013, 20, 2
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The occurrence of Toxoplasma gondii infection in people and animals from rural environment of Lublin region - estimate of potential role of water as a source of infection
    Autorzy:
    Sroka, J
    Wojcik-Fatla, A.
    Szymanska, J.
    Dutkiewicz, J.
    Zajac, V.
    Zwolinski, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/49789.pdf
    Data publikacji:
    2010
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    parasite
    protozoan parasite
    Toxoplasma gondii
    occurrence
    people
    animal
    domestic animal
    rural environment
    Lublin region
    epidemiology
    serological examination
    farm inhabitant
    potable water
    polymerase chain reaction
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2010, 17, 1; 125-132
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Distribution of trichothecene and zearalenone producing Fusarium species in grain of different cereal species and cultivars grown under organic farming conditions in Lithuania
    Autorzy:
    Suproniene, S
    Justesen, A.F.
    Nicolaisen, M.
    Mankeviciene, A.
    Dabkevicius, Z.
    Semaskiene, R.
    Leistrumaite, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/49864.pdf
    Data publikacji:
    2010
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    Lithuania
    organic farming
    farming condition
    plant cultivar
    cereal
    real-time polymerase chain reaction
    Fusarium
    Fusarium langsethiae
    trichothecene
    zearalenone
    distribution
    human health
    animal health
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2010, 17, 1; 79-86
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Prevalence of Anaplasma phagocytophilum in Ixodes ricinus ticks determined by polymerase chain reaction with two pairs of primers detecting 16S rRNA and ankA genes
    Autorzy:
    Chmielewska-Badora, J
    Zwolinski, J.
    Cisak, E.
    Wojcik-Fatla, A.
    Buczek, A.
    Dutkiewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/51023.pdf
    Data publikacji:
    2007
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    Lublin region
    Polska
    pathogen
    Ixodes ricinus
    Eastern Poland
    tick
    determination
    animal pathogen
    human pathogen
    ankA gene
    16S rRNA gene
    granulocytic anaplasmosis
    bacteria
    Anaplasma phagocytophilum
    polymerase chain reaction
    Opis:
    A total of 684 Ixodes ricinus ticks (321 nymphs, 184 males, and 179 females) were collected by fl agging lower vegetation in 6 forest districts located on the territory of Lublin province (eastern Poland). Ticks were examined by polymerase chain reaction (PCR) method for the presence of Anaplasma phagocytophilum DNA with two pairs of primers: EHR521/EHR747 for detecting 16S rRNA gene, and LA6/LA1 for detecting ankA gene. To study the relationship between infection in ticks and people occupationally exposed to tick bite, blood serum samples of 261 forestry workers employed in the same forest districts were examined by immunofl uorescence method for the presence of specifi c antibodies against A. phagocytophilum. A total of 70 ticks out of 684 examined (10.2%) showed the presence of A. phagocytophilum 16S rRNA gene. The prevalence of infection was signifi cantly dependent on tick’s stage (χ2=49.2, p<0.00001) and geographical locality (χ2=34.4, p<0.00001). The percentage of I. ricinus females infected with A. phagocytophilum (24.6%) was signifi cantly greater compared to males (6.5%) and nymphs (4.4%) (p<0.00001). Only 19 ticks out of 684 examined (2.8%) showed the presence of A. phagocytophilum ankA gene, signifi cantly less compared to 16S rRNA gene (p<0.00001). The prevalence of infection demonstrated by the presence of ankA gene was also signifi cantly dependent on tick’s stage (χ2=23.6, p<0.00001) but not on locality (χ2=9.8, p=0.082). A signifi cant correlation was found between the presence of A. phagocytophilum 16S rRNA gene in I. ricinus female ticks from the particular forest districts and the serologic response to A. phagocytophilum of forestry workers employed in the same districts (p<0.05). No signifi cant correlation was found between the presence of A. phagocytophilum ankA gene in I. ricinus ticks and serologic response of exposed workers. In conclusion, detection of A. phagocytophilum infection in ticks by PCR with the use of EHR521/EHR747 primers detecting 16S rRNA gene is signifi cantly more sensitive compared to LA6/LA1 primers detecting ankA gene.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2007, 14, 2
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-15 z 15

      Ta witryna wykorzystuje pliki cookies do przechowywania informacji na Twoim komputerze. Pliki cookies stosujemy w celu świadczenia usług na najwyższym poziomie, w tym w sposób dostosowany do indywidualnych potrzeb. Korzystanie z witryny bez zmiany ustawień dotyczących cookies oznacza, że będą one zamieszczane w Twoim komputerze. W każdym momencie możesz dokonać zmiany ustawień dotyczących cookies