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    Wyświetlanie 1-2 z 2
    Tytuł:
    The viability and intestinal epithelial cell adhesion of probiotic strain combination - in vitro study
    Autorzy:
    Piatek, J.
    Gibas-Dorna, M.
    Olejnik, A.
    Krauss, H.
    Wierzbicki, K.
    Zukiewicz-Sobczak, W.
    Glowacki, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/52041.pdf
    Data publikacji:
    2012
    Wydawca:
    Instytut Medycyny Wsi
    Opis:
    To be effective, probiotic bacteria must exhibit a number of functional characteristics, including the resistance to gastric acidity and the ability to adhere to the intestinal epithelium. In this study, we examined in vitro the viability of lactic acid bacteria (LAB) combination after exposure to low pH, and the adhesion of LAB to Caco-2 cells during coincubation of 9 bacterial strains. To test bacterial viability, 6 commercially available products were incubated in 0.1 N HCl at pH 1.2 for 60 min. The greatest growth inhibition was noted for the non-capsulated product containing the Lactobacillus rhamnosus strain (log reduction of CFU = 6.4), and the best survival observed for the product containing 9 bacterial strains, equipped with a modern capsule made according to the Multi-Resistant Encapsulation technology (log reduction of CFU = 0.1). In the adhesion experiment, the combination of 9 bacterial strains was added to 17-day-old Caco-2 cell culture for 90 min. The greatest efficiency of adhesion was observed for the inoculum containing 5.5 × 108 CFU/mL/9.6 cm2 of Caco-2 and the dose of probiotic bacteria of 190 cells per one Caco-2 cell. As a result, approximately 157 bacterial cells adhered to one Caco-2 cell. The results indicate that the combination of 9 bacterial strains in the examined product is characterized as highly adhesive.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2012, 19, 1
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Occurrence of neurotic and anxiety disorders in rural schoolchildren and the role of physical exercise as a method to support their treatment
    Autorzy:
    Krauss, H.
    Buraczynska-Andrzejewska, B.
    Piatek, J.
    Sosnowski, P.
    Mikrut, K.
    Glowacki, M.
    Misterska, E.
    Zukiewicz-Sobczak, W.
    Zwolinski, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/50344.pdf
    Data publikacji:
    2012
    Wydawca:
    Instytut Medycyny Wsi
    Opis:
    Introduction: School anxiety has become a common problem in children and adolescents. Despite numerous studies, it is difficult to definitely identify the cause of neurotic disorders in school-age children. The objective of the presented study was to assess the prevalence of neurotic disorders in rural schoolchildren and the role of physical activity as a method to support their treatment. Material and methods: The study consisted of 123 girls and 117 boys living in rural areas of Wielkopolska. A questionnaire was provided evaluating family status, family relationships, school situation, somatic symptoms related to being at school, and the frequency of physical activity. Results: It was found that over the 50% of children considered the relations with siblings as correct, but also acknowledged that there were a conflict situations in their relationship. Nearly 25% of children indicated an emotional irrelevance with their parents. About 20% of the young people did not like going to school or were afraid to stay there. Over the 50% of children declared somato-emotional problems associated with the being at school. The symptoms of neurotic disorders were more strongly expressed and more common in girls. Analysis of physical activity indicated that only a small group of young people practiced sport regularly, and an even smaller percentage of children exercised with their parents and/or peers. Conclusions: The development of somatic and emotional disturbances in children may be associated with irregular family and school relationships and low physical activity. It was found that gender had a significant influence on most of the studied parameters.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2012, 19, 3
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-2 z 2

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