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Wyszukujesz frazę "Kubiak, L." wg kryterium: Wszystkie pola


Wyświetlanie 1-2 z 2
Tytuł:
Microbiological analysis of bioaerosols collected from Hospital Emergency Departments and ambulances
Autorzy:
Bielawska-Drózd, A.
Cieślik, P.
Bohacz, J.
Kornillowicz-Kowalska, T.
Żakowska, D.
Bartoszcze, M.
Wlizło-Skowronek, B.
Winnicka, I.
Brytan, M.
Kubiak, L.
Skopińska-Różewska, E.
Kocik, J.
Powiązania:
https://bibliotekanauki.pl/articles/2081735.pdf
Data publikacji:
2018
Wydawca:
Instytut Medycyny Wsi
Tematy:
Button Sampler
bioaerosol
Hospital Emergency Departments
ambulances
bacteria
fungi
Opis:
Introduction and objective. The goal of the study was a microbiological, qualitative and quantitative analysis of bioaerosol at the workplace of medical personnel (Health Emergency Departments (HEDs), ambulances), and comparative administration offices with an expected neutral occupational exposure to biological agents measured with individual Button Sampler. Materials and method. Personal sampling was performed with Button Sampler instrument loaded with gelatine filters in 10 HEDs, in 9 ambulances and in 9 offices to assess the occupational biological agents’ exposure in air. Sampling was conducted from March until April 2016. Samples were quantitatively assessed for viable and total number of bacteria and fungi. Routine procedures for microbiological diagnostics were implemented. Data were analysed using Kruskal–Wallis and Mann-Whitney statistical tests with α=0.05. P value less than 0.05 were considered significant. Results. At the workplaces assessed, the concentrations of viable microorganisms in HEDs were 1.3 × 102 – 4.2 × 103 CFU/m3 for bacteria, 3.4 × 100 – 8.1 × 101 CFU/m3 for fungi; in ambulances 1.3 × 102 – 1.4 × 103 CFU/m3 (bacteria), 6.7 × 100 – 6.5 × 102 CFU/m3 (fungi) and in offices 4.2 × 101 – 5.0 × 103 CFU/m3 (bacteria), 0 – 7.9 × 102 CFU/m3 (fungi). In outdoor air, the number of microorganisms reached the level: 1.0 × 102 – 5.9 × 102 CFU/m3 for bacteria and 1.5 × 102 – 8.2 × 102 CFU/m3 for fungi. The predominant isolated bacteria were Gram-positive cocci. The prevalent fungi species belonged to the genus Aspergillus and Penicillium. Conclusions. The quantitative assessment of examined indoor air was similar to control outdoor air, and were relatively low. The level of microbiological contamination did not exceed 5 × 103 CFU/m3 which is recommended as an admissible level in public spaces in Poland.
Źródło:
Annals of Agricultural and Environmental Medicine; 2018, 25, 2; 274-279
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Severe Influenza Outbreak in Western Ukraine in 2009 – a molecular-epidemiological study
Autorzy:
Kocik, J.
Niemcewicz, M.
Johns, M.
Jerke, K.
Michalski, A.
Bielecka, A.
Lasocki, K.
Gawel, J.
Bielawska-Drozd, A.
Joniec, J.
Kolodziej, M.
Graniak, G.
Goniewicz, M.
Kubiak, L.
Powiązania:
https://bibliotekanauki.pl/articles/50555.pdf
Data publikacji:
2013
Wydawca:
Instytut Medycyny Wsi
Opis:
Introduction: In the autumn of 2009 the authors participated in a humanitarian operation in Western Ukraine by undertaking an epidemiological investigation of an influenza-like-illness (ILI) in the L’viv Oblast region. Mobile biological survey teams took samples from civilian patients with severe acute respiratory distress syndrome, rapid transportation of the samples, and their molecular analysis in Poland to provide accurate results. Objective: The aim of the study was the molecular and epidemiological analysis of the biological samples collected. Material and Methods: Real-time reverse transcriptase polymerase chain reaction (real-time RT-PCR), multiplex PCR techniques, traditional Sanger Sequencing and classical viral culture methods were used. Results: Among the 124 influenza-like illness cases, ~50% (58) were positive for influenza A virus in WHO-CDC molecular assay, including subtyping. The specimens were further analyzed to confirm results and determine the genetic sequence. Phylogenetically, the nucleotide similarity of both the Ukraine specimens and reference A/California/7/2009 (pH1N1) was 99.2–99.3%. Oseltamivir resistance was not registered. HA1 region characterization showed an overall protein identity of 98.5–99.4%. Conclusions: An unexpected high contribution of influenza A was confirmed among ILI patients, as well as a very limited number of other detected viruses, indicate that the 2009 epidemic in western Ukraine was strongly related to novel influenza A/H1N1. The importance of swift sharing of information and reference laboratories networking in surveillance, as well as serving governments and international agencies in pursuing adequate actions, should be stressed.
Źródło:
Annals of Agricultural and Environmental Medicine; 2013, 20, 3
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-2 z 2

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