- Tytuł:
- The influence of selective COX-2 inhibitor on phase of healing surgical wounds: proliferation and secretion of bFGF by endothelial cells
- Autorzy:
-
Jasiak, Łukasz
Kowalczyk, Mateusz
Mazan, Paula
Kowalczyk, Edward
Sienkiewicz, Monika
Jóźwiak-Bębnista, Marta
Wiktorowska-Owczarek, Anna - Powiązania:
- https://bibliotekanauki.pl/articles/763865.pdf
- Data publikacji:
- 2017
- Wydawca:
- Uniwersytet Marii Curie-Skłodowskiej. Wydawnictwo Uniwersytetu Marii Curie-Skłodowskiej
- Tematy:
- angiogenesis, selective COX-2 inhibitor, fibroblast growth factor, vascular endothelial cell
- Opis:
- The process of wound healing consists of the following phases: inflammation, proliferation, remodeling. Non-steroidal antiinflammatory drugs may be important in this process, especially in a stage called angiogenesis. For this reason, it was decided to investigate the effect of selective COX-2 (cyclooxygenase 2) inhibitor (NS-398) on the proliferation of endothelial cells and their ability to secrete bFGF (fibroblast growth factor) for vascular endothelial cells (HMEC-1). For determination of the secretion of bFGF in a cell line HMEC-1 immunosorbent ELISA assays were used. In turn, the cell proliferation assay was performed using the MTT method. Using MTT method, it was found that NS-398 at 10 μM did not affect cell viability. Whereas selective COX-2 inhibitor at 100 μM decreased cell viability in a statistically significant manner and inhibited the proliferative effect of 100 μg/mL LPS at concentrations of 10 and 100 μM. In the further step, application of NS-398 (10 and 100 μM) with LPS (100 μg/mL; inflammatory environment) reduced the secretion of bFGF in a statistically significant manner. The investigations showed that NS-398 has an antiangiogenic effect which is based on reducing the proliferation of vascular endothelial cells and inhibiting the secretion of bFGF- factor responsible for angiogenesis during wound healing.
- Źródło:
-
Annales Universitatis Mariae Curie-Sklodowska, sectio C – Biologia; 2017, 72, 1
2083-3563
0066-2232 - Pojawia się w:
- Annales Universitatis Mariae Curie-Sklodowska, sectio C – Biologia
- Dostawca treści:
- Biblioteka Nauki
Artykuł