- Tytuł:
- The membrane with polylactide and hyaluronic fibers for skin substitute
- Autorzy:
-
Stodolak-Zych, E.
Rozmus, K.
Dzierzkowska, E.
Zych, Ł.
Kapacz-Kmita, A.
Gargas, M.
Kołaczkowska, E.
Cieniawska, M.
Książek, A.
Ścisłowska-Czarnecka, A. - Powiązania:
- https://bibliotekanauki.pl/articles/306599.pdf
- Data publikacji:
- 2018
- Wydawca:
- Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
- Tematy:
-
nanowłókna
elektroprzędzenie
kwas hialuronowy
polilaktyd
nanofibrous scaffold
electrospinning
skin substitute
hyaluronic acid
polylactide - Opis:
- Skin substitutes are heterogeneous group of scaffolds (natural or synthetic) and cells. We hypothesize that nanofibers with layer composition made of polylactide (PLA) and sodium hyaluronate (HA) obtained using electrospinning method are a good matrix for cell adhesion and proliferation. Methods: Optimal conditions of electrospinning of PLA and HA nanofibers to create layered compositions (PLA membrane covered with HA nonwovens) were determined by modifying parameters such as the appropriate amount of solvents, polymer concentration, mixing temperature and electrospinning process conditions. By changing the parameters, it was possible to control the diameter and properties of both polymer fibers. The spinning solution were characterized by surface tension and rheology. A scanning electron microscope (SEM) was used to determine the morphology and fiber diameters: PLA and HA. Structure of the PLA/HA nonwoven was analyzed using spectroscopy (FTIR/ATR). Biocompatibility of the nonwoven with fibroblasts (ECM producers) was assessed in the in vitro conditions. Results: The results showed that stable conditions for the formation of submicron PLA fibers were obtained using a 13% wt. solution of the polymer, dissolved in a 3:1 mixture of DCM:DMF at 45 °C. The hyaluronic fibers were prepared from a 12% wt. solution of the polymer dissolved in a 2:1 mixture of ammonia water and ethyl alcohol. All materials were biocompatible but to a different degree. Conclusions: The proposed laminate scaffold was characterized by a hydrophobic-hydrophilic domain surface with a maintained fiber size of both layers. The material positively underwent biocompatibility testing in contact with fibroblasts.
- Źródło:
-
Acta of Bioengineering and Biomechanics; 2018, 20, 4; 91-99
1509-409X
2450-6303 - Pojawia się w:
- Acta of Bioengineering and Biomechanics
- Dostawca treści:
- Biblioteka Nauki
Artykuł