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    Wyświetlanie 1-3 z 3
    Tytuł:
    De novo sequencing and comparative transcriptome analysis of white petals and red labella in Phalaenopsis for discovery of genes related to flower color and floral differentiation
    Autorzy:
    Yang, Y.
    Wang, J.
    Ma, Z.
    Sun, G.
    Zhang, C.
    Powiązania:
    https://bibliotekanauki.pl/articles/58306.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    sequencing
    RNA sequence
    transcriptome
    Phalaenopsis
    gene
    flower
    colour
    floral differentiation
    diversity
    Opis:
    Phalaenopsis is one of the world’s most popular and important epiphytic monopodial orchids. The extraordinary floral diversity of Phalaenopsis is a reflection of its evolutionary success. As a consequence of this diversity, and of the complexity of flower color development in Phalaenopsis, this species is a valuable research material for developmental biology studies. Nevertheless, research on the molecular mechanisms underlying flower color and floral organ formation in Phalaenopsis is still in the early phases. In this study, we generated large amounts of data from Phalaenopsis flowers by combining Illumina sequencing with differentially expressed gene (DEG) analysis. We obtained 37 723 and 34 020 unigenes from petals and labella, respectively. A total of 2736 DEGs were identified, and the functions of many DEGs were annotated by BLAST-searching against several public databases. We mapped 837 up-regulated DEGs (432 from petals and 405 from labella) to 102 Kyoto Encyclopedia of Genes and Genomes pathways. Almost all pathways were represented in both petals (102 pathways) and labella (99 pathways). DEGs involved in energy metabolism were significantly differentially distributed between labella and petals, and various DEGs related to flower color and floral differentiation were found in the two organs. Interestingly, we also identified genes encoding several key enzymes involved in carotenoid synthesis. These genes were differentially expressed between petals and labella, suggesting that carotenoids may influence Phalaenopsis flower color. We thus conclude that a combination of anthocyanins and/or carotenoids determine flower color formation in Phalaenopsis. These results broaden our understanding of the mechanisms controlling flower color and floral organ differentiation in Phalaenopsis and other orchids.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2014, 83, 3
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Revealing the core transcriptome modulating plant growth phase in Arabidopsis thalianaby RNA sequencingand coexpression analysis of the FHY3 FAR1 mutant
    Autorzy:
    Wang, W.
    Zhao, G.
    Wu, S.
    Hua, W.
    Zhang, T.
    Ruan, R.
    Cheng, Y.
    Powiązania:
    https://bibliotekanauki.pl/articles/2130692.pdf
    Data publikacji:
    2020
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    weekly transcriptome analysis
    plant development
    different expression genes
    (DEGs)
    immune response
    Opis:
    Plants must continually calibrate their growth in response to the environment throughout their whole life cycle. Revealing the regularity of plant early growth and development is of great significance to plant genetic modification. It was previously demonstrated that loss of two key light signaling transcription factors, FHY3 and FAR1, can cause a stunted stature in the plant adult stage, and numerous defense response genes can be continuously activated. In this study, we performed a time-course transcriptome analysis of the early 4 weeks of leaf samples from wild plants and their fhy3 and far1 transcription factors. By comparative transcriptome analysis, we found that during the early 4 weeks of plant growth, plants primarily promoted morphogenesis by organizing their microtubules in the second week. In the third week, plants began to trigger large- scale defense responses to resist various external stresses. In the fourth week, increased photosynthetic efficiency promoted rapid biomass accumulation. Weighted gene coexpression network analysis of FHY3 and FAR1 revealed that the two light signaling transcription factors may be originally involved in the regulation of genes during embryonic development, and in the later growth stage, they might regulate gene expression of some defense-related genes to balance plant growth and immunity. Remarkably, our yeast two-hybrid and bimolecular fluorescence complementation experiments showed that FAR1 interacts with the immune signaling factor EDS1. Taken together, this study demonstrates the major biological processes occurring during the early 4 weeks of plant growth. The light signaling transcription factors, FHY3 and FAR1, may integrate light signals with immune signals to widely regulate plant growth by directly interacting with EDS1.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2020, 89, 2
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Isolation of biosynthesis related transcripts of 2,3,5,4-tetrahydroxy stilbene-2-O-beta-D-glucoside from Fallopia multiflora by suppression subtractive hybridization
    Autorzy:
    Zhao, W.
    Sheng, S.
    Liu, Z.
    Lu, Di.
    Zhu, K.
    Li, X.
    Zhao, S.
    Yao, Y.
    Powiązania:
    https://bibliotekanauki.pl/articles/58239.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    isolation
    biosynthesis
    transcriptome
    2,3,5,4'-tetrahydroxy stilbene-2-O-beta-D-glucoside
    Fallopia multiflora
    hybridization
    gene expression
    medicinal plant
    Chinese herb
    Opis:
    2,3,5,4'-tetrahydroxy stilbene-2-O-ß-D-glucoside (THSG) exerts multiple pharmacodynamic actions, found in Fallopia multiflora, but the biosynthesis pathway of THSG is still unclear. To clear this ambiguity, we constructed suppression subtractive hybridization (SSH) libraries to screen the genes involved in THSG biosynthesis from two F. multiflora varieties, which vary significantly in THSG content. Twelve non-redundant differentially expressed sequence tags were obtained and the full lengths of 4 unreported fragments were amplified by rapid amplification of cDNA ends. We totally got 7 fulllength transcripts, and all of them were aligned to the transcriptome and digital gene expression tag profiling database of four F. multiflora tissues (root, stem and leaf from Deqing F. multiflora and another root from Chongqing F. multiflora; data unpublished) using local BLAST. The results showed that there was a significant, organ specific difference in the expression of fragments and full-length sequences. All the sequences were annotated by aligning to nucleotide and protein databases. Kyoto Encyclopedia of Genes and Genomes pathway analysis indicated that THSG biosynthesis was correlated with multiple life activities.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2014, 83, 2
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-3 z 3

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