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Wyświetlanie 1-2 z 2
Tytuł:
In vitro cultures of Salvia officinalis L. as a source of antioxidant compounds
Autorzy:
Grzegorczyk, I
Bilichowski, I.
Mikiciuk-Olasik, E.
Wysokinska, H.
Powiązania:
https://bibliotekanauki.pl/articles/57164.pdf
Data publikacji:
2005
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
Salvia officinalis
carnosic acid
concentration
rosmarinic acid
sage
botany
antioxidant compound
in vitro
carnosol
Opis:
The concentrations of carnosic acid, carnosol and rosmarinic acid in different materials from differentiated (multiple shoot cultures and regenerated plants) and undifferentiated (callus and cell suspension) in vitro cultures of Salvia officinalis were determined by HPLC. The results suggested that diterpenoid (carnosic acid and carnosol) production is closely related to shoot differentiation. The highest diterpenoid yield (11.4 mg g-1 for carnosic acid and 1.1 mg g-1 for carnosol) was achieved in shoots of 10-week-old micropropagated plants. The levels were comparable to those found in shoots of naturally growing plants. Undifferentiated callus and cell suspension cultures produced only very low amounts of carnosol (ca. 0.05 mg g-1 of dry weight). In contrast, content of rosmarinic acid in callus and suspension cultures as well as shoots growing in vitro and in vivo was similar and ranged between 11.2 and 18.6 mg g-1 of dry weight.
Źródło:
Acta Societatis Botanicorum Poloniae; 2005, 74, 1
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Liquid shoot culture of Salvia officinalis L. for micropropagation and production of antioxidant compounds; Effect of triacontanol
Autorzy:
Grzegorczyk, I
Wysokinska, H.
Powiązania:
https://bibliotekanauki.pl/articles/58539.pdf
Data publikacji:
2008
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
Salvia officinalis
medicinal plant
triacontanol
liquid shoot culture
botany
herb
micropropagation
production
antioxidant compound
sage
Opis:
Liquid shoot culture of Salvia officinalis L. in MS medium containing IAA (0.1 mg l-1) and BAP (0.45 mg l-1) was developed and evaluated in relation to shoot multiplication and antioxidant compound (carnosic acid, carnosol and rosmarinic acid) accumulation. In the liquid medium, on average, 3 new shoots per explant (shoot tip) were obtained within 3 weeks. The shoots produced 8.2±0.02 mg of diterpenoids and 31.2±0.29 mg of rosmarinic acid per gram of dry weight. Shoot proliferation and diterpenoid content increased when triacontanol (5, 10 or 20 pg l-1) was added to the liquid medium. In optimum conditions (at 20 pg l-1 TRIA) almost 7 shoots were formed per explant after 3 weeks. An increase in diterpenoid production (expressed as the sum of carnosol and carnosic acid) ranged from 30% to 50% and dependended on triacontanol concentration tested. The level of diterpenoids in triacontanol-treated shoots was similar to the content of compounds in commercial herbal product (dried leaves of S. officinalis) (10-12 mg g-1 dry wt). Triacontanol did not increase rosmarinic acid production, but the content of the phenolic as compound in shoots grown in liquid culture (31 mg g-1 dry wt) was even 24 times higher compared to samples of dried leaves of S. officinalis plants. We also demonstrated that the highest amounts of CA, Car and RA were accumulated in young, top parts of sage shoots. This observation could be useful for improving the selection of material for the extraction of natural antioxidants from S. officinalis.
Źródło:
Acta Societatis Botanicorum Poloniae; 2008, 77, 2
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-2 z 2

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