- Tytuł:
- Evaluation of Intracellular Ca2+ Concentration by Fura 2 Ratiometry in Encystment-induced Colpoda cucullus
- Autorzy:
- Matsuoka, Tatsuomi
- Powiązania:
- https://bibliotekanauki.pl/articles/763521.pdf
- Data publikacji:
- 2013
- Wydawca:
- Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
- Tematy:
- Colpoda, Ca2+, encystment induction, fura 2
- Opis:
- In Colpoda cucullus, the signaling pathways for encystment induction involving protein phosphorylation have been believed to be triggered by an increase in the intracellular Ca2+ concentration promoted by cell-to-cell mechanical contact due to overpopulation. By means of fura 2 ratiometry, the present study showed that the intracellular Ca2+ concentration was actually elevated when vegetative cells were induced to encyst by being suspended at a high cell density in the presence of external free Ca2+ and suppressed by chelating external Ca2+. This result strongly suggests that an increase in the intracellular Ca2+ concentration caused by an inflow of Ca2+ promoted by cell-tocell mechanical contact due to overpopulation enhances the rate of encystation in Colpoda cucullus.
- Źródło:
-
Acta Protozoologica; 2013, 52, 1
1689-0027 - Pojawia się w:
- Acta Protozoologica
- Dostawca treści:
- Biblioteka Nauki
Artykuł