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Wyszukujesz frazę "photodynamic therapy" wg kryterium: Temat

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    Wyświetlanie 1-15 z 15
    Tytuł:
    Tritolylporphyrin dimer as a new potent hydrophobic sensitizer for photodynamic therapy of melanoma.
    Autorzy:
    Drzewiecka, Agnieszka
    Urbańska, Krystyna
    Matuszak, Zenon
    Pineiro, Marta
    Arnaut, Luis
    Habdas, Jan
    Ratuszna, Alicja
    Stochel, Grażyna
    Powiązania:
    https://bibliotekanauki.pl/articles/1044198.pdf
    Data publikacji:
    2001
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    melanoma
    porphyrins
    photodynamic therapy
    Opis:
    We report the synthesis, photochemical and photophysical properties and preliminary studies on biological effect of a new tritolylporphyrin dimer (T-D). Absorption and emission properties of T-D suggest its possible use in photodynamic therapy. T-D is capable of singlet oxygen production with 0.8 quantum yield. It also has a high photostability. The photodynamic properties of the dimer were examined following the growth of SKMEL 188 (human melanoma) cells irradiated with red light (cut off <630 nm). The surviving fraction of the cells decreased about 3-fold (vs. non-irradiated cells) for an 81 J/cm2 dose. Our results suggest that tritolylporphyrine dimer T-D may be an interesting hydrophobic sensitizer for photodynamic therapy.
    Źródło:
    Acta Biochimica Polonica; 2001, 48, 1; 277-282
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Photodynamic effect of lanthanide derivatives of meso-tetra(N-methyl-4-pyridyl)porphine against Staphylococcus aureus
    Autorzy:
    Jurczak, Agata
    Szramka, Bozena
    Grinholc, Mariusz
    Legendziewicz, Janina
    Bielawski, Krzysztof
    Powiązania:
    https://bibliotekanauki.pl/articles/1040720.pdf
    Data publikacji:
    2008
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    photosensitizer
    porphyrin
    photodynamic therapy
    MRSA
    Opis:
    Photodynamic therapy (PDT), used for cancer treatment, is also an alternative method for eradication of drug-resistant bacteria. This method utilizes a nontoxic light-activated dye, called a photosensitizer, and visible light to produce reactive oxygen species that lead to bacterial cell death. The purpose of this study was to investigate the bactericidal effect of PDT using lanthanide derivatives of meso-tetra(N-methyl-4-pyridyl)porphine against Staphylococcus aureus strains. The new photosensitizers appeared to be photodynamically ineffective. No enhancement of antistaphylococcal activity of TMPyP was observed after the conjugation of the porphyrin with lanthanide ions. Additionally, a significant difference in the susceptibility of two bacterial strains to unmodified TMPyP was observed.
    Źródło:
    Acta Biochimica Polonica; 2008, 55, 3; 581-585
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Bactericidal effect of photodynamic therapy against methicillin-resistant Staphylococcus aureus strain with the use of various porphyrin photosensitizers
    Autorzy:
    Grinholc, Mariusz
    Szramka, Bozena
    Olender, Katarzyna
    Graczyk, Alfreda
    Powiązania:
    https://bibliotekanauki.pl/articles/1041060.pdf
    Data publikacji:
    2007
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    photosensitizer
    porphyrin
    photodynamic therapy
    MRSA
    Opis:
    Photodynamic therapy (PDT) is based on photosensitizers activated by light of appropriate wavelength. Their activation leads to generation of singlet oxygen and free radicals responsible for the cytotoxic effect. The aim of this project was to compare the bactericidal effect of PDT using different porphyrin photosensitizers against a methicillin-resistant Staphylococcus aureus strain. Exogenous sensitizers (protoporphyrin IX and newly synthesized derivative, protoporphyrin diarginate) induced a 3 log10-unit reduction in bacterial viable counts. With the use of endogenous, ALA-induced porphyrins, a 1.6 log10-unit reduction was obtained. The sensitizers tested executed their antibacterial activity with no essential change in the antibiotic resistance pattern of the studied strain.
    Źródło:
    Acta Biochimica Polonica; 2007, 54, 3; 665-670
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Direct tumor damage mechanisms of photodynamic therapy.
    Autorzy:
    Nowis, Dominika
    Makowski, Marcin
    Stokłosa, Tomasz
    Legat, Magdalena
    Issat, Tadeusz
    Gołąb, Jakub
    Powiązania:
    https://bibliotekanauki.pl/articles/1041411.pdf
    Data publikacji:
    2005
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    photosensitizer
    tumor
    photodynamic therapy
    apoptosis
    Opis:
    Photodynamic therapy (PDT) is a clinically approved and rapidly developing cancer treatment regimen. It is a minimally invasive two-stage procedure that requires administration of a photosensitizing agent followed by illumination of the tumor with visible light usually generated by laser sources. A third component of PDT is molecular oxygen which is required for the most effective antitumor effects. In the presence of the latter, light of an appropriate wavelength excites the photosensitizer thereby producing cytotoxic intermediates that damage cellular structures. PDT has been approved in many countries for the treatment of lung, esophageal, bladder, skin and head and neck cancers. The antitumor effects of this treatment result from the combination of direct tumor cell photodamage, destruction of tumor vasculature and activation of an immune response. The mechanisms of the direct photodamage of tumor cells, the signaling pathways that lead to apoptosis or survival of sublethaly damaged cells, and potential novel strategies of improving the antitumor efficacy of PDT are discussed.
    Źródło:
    Acta Biochimica Polonica; 2005, 52, 2; 339-352
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Application of electrophoretic methods for detection of protein-porphyrin complexes.
    Autorzy:
    Kowalska, Agnieszka
    Kwiek, Piotr
    Miłosz, Ewa
    Gondek, Grzegorz
    Romiszewska, Anna
    Graczyk, Alfreda
    Podhajska, Anna
    Powiązania:
    https://bibliotekanauki.pl/articles/1043407.pdf
    Data publikacji:
    2003
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    porphyrins
    photodynamic therapy
    PDT
    electrophoresis
    Opis:
    Simple methods for detection and isolation of protein-porphyrin complexes were elaborated in our laboratory. They are based on the separation of protein-porphyrin complexes in native polyacrylamide gel and measurement of their fluorescence, with the use of two detection systems: the commercially available Gel DocTM 2000 system, and a system specially designed for the purpose of these investigations, concerning protein-porphyrin interactions. The fluorescent complexes can be electro-transferred from the gel onto PVDF membrane, eluted and analyzed in order to identify the protein interacting with porphyrins.
    Źródło:
    Acta Biochimica Polonica; 2003, 50, 4; 1155-1163
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Indocyanine green as a prospective sensitizer for photodynamic therapy of melanomas.
    Autorzy:
    Urbanska, Krystyna
    Romanowska-Dixon, Bożena
    Matuszak, Zenon
    Oszajca, Janusz
    Nowak-Sliwinska, Patrycja
    Stochel, Grażyna
    Powiązania:
    https://bibliotekanauki.pl/articles/1043767.pdf
    Data publikacji:
    2002
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    melanoma
    photodynamic therapy
    indocyanine green
    Opis:
    Spectroscopic, photochemical and biological properties of indocyanine green (ICG) are presented. Light over 800 nm is effectively absorbed by ICG. This property as well as photochemical behaviour of ICG make it a very suitable dye for photodynamic treatment of melanoma cells. Cytotoxicity of ICG itself and the effect of photodynamic therapy (PDT) were evaluated by following the growth of human (SKMEL 188) and mouse (S91) melanoma cells. The surviving fraction of the cells irradiated (λex = 830 nm) vs non-irradiated, treated with the same dose of ICG, is significantly decreased (5- to 10-fold). These results show that ICG is a very promising dye for photodynamic therapy of melanomas.
    Źródło:
    Acta Biochimica Polonica; 2002, 49, 2; 387-391
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Combined effects of radiotherapy and photodynamic therapy on an in vitro human prostate model.
    Autorzy:
    Colasanti, Alberto
    Kisslinger, Annamaria
    Quarto, Maria
    Riccio, Patrizia
    Powiązania:
    https://bibliotekanauki.pl/articles/1041520.pdf
    Data publikacji:
    2004
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    radiotherapy
    prostate
    photodynamic therapy
    indocyanine green
    Opis:
    Human prostate cancer cells were evaluated for growth after photodynamic therapy, radiotherapy, and combined treatment. Indocyanine green was tested as a photosensitizer and radiosensitizer. Two human cell lines were used: PC-3 derived from prostate carcinoma, and EPN derived from normal prostate tissue. The light source used for the photoactivation experiments was a diode laser peaked at 805 nm. The light dose incident on cells was 108 J/cm2. Ionizing radiation was produced by a linear accelerator, and the dose was 2, 4 and 6 Gy. Cytotoxicity was evaluated by measuring the colony forming ability of cells. Our results show that indocyanine green induces cell death by photoactivation, but it does not act as a radiosensitizer if used with ionizing radiation. The combined treatment of photodynamic therapy and radiotherapy produces an additive effect which does not depend on the sequence of the two treatments. Combined treatments could be more useful since they allow the reduction of the ionizing radiation dose to obtain the same effect as one obtainable by radiotherapy alone.
    Źródło:
    Acta Biochimica Polonica; 2004, 51, 4; 1039-1046
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Uptake of photofrin II, a photosensitizer used in photodynamic therapy, by tumour cells in vitro
    Autorzy:
    Chwiłkowska, Agnieszka
    Saczko, Jolanta
    Modrzycka, Teresa
    Marcinkowska, Anna
    Malarska, Anna
    Bielewicz, Julita
    Patalas, Dorota
    Banaś, Teresa
    Powiązania:
    https://bibliotekanauki.pl/articles/1043629.pdf
    Data publikacji:
    2003
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    photofrin II
    photodynamic therapy
    fluorescence spectra
    Opis:
    Photosensitizing dyes are used in fluorescence diagnostics and photodynamic therapy (PDT). These usually hematoporphyrin derivatives (HpD) accumulate preferentially within neoplastic tissues. HpD is a mixture of ether and ester linked porphyrins. Its partially purified form is known under the commercial name of photofrin II (PII). PII emission spectra were studied in a hydrophilic (PBS) and a lipophilic (PC liposomes) environment. Red shift was observed in their emission maxima from 615 nm in buffer solution to 635 nm in lipid. Identical red shift was obtained when the intracellular fluorescence of two cancer cell lines, MCF 7 and Jurkat, incubated with PII was investigated. Thus, intramembrane localization of PII may be suggested. As determined from the total fluorescence intensity, the uptake of PII was only slightly higher for Jurkat than for MCF 7 cells. Nevertheless the kinetics of the uptake was found to be different for both cell lines.
    Źródło:
    Acta Biochimica Polonica; 2003, 50, 2; 509-513
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Cytotoxicity of PP(Arg)2- and PP(Ala)2(Arg)2-based photodynamic therapy and early stage of apoptosis induction in human breast cancers in vitro
    Autorzy:
    Nowak-Stępniowska, Agata
    Wiktorska, Katarzyna
    Małecki, Maciej
    Milczarek, Małgorzata
    Lubelska, Katarzyna
    Padzik-Graczyk, Alfreda
    Powiązania:
    https://bibliotekanauki.pl/articles/1039664.pdf
    Data publikacji:
    2012
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    photodynamic therapy
    breast cancer
    transmembrane mitochondrial potential
    Opis:
    Mitochondria are cell energetic centers where ATP is produced. They also play a very important role in the PDT as intracellular sites of photosensitizer localization. Photosensitizers gathering in mitochondria (like porphyrin derivatives used in this work) are more effective in tumor cell destruction. Moreover, it was assumed that di-amino acid substituents attached to porphyrin ring will strengthen the effectivity of interaction with membrane receptors of examined cells. MTT assay was performed to investigate the influence of PP(Arg)2 and PP(Ala)2(Arg)2-based PDT on breast cancer cell viability for 24 h, 48 h and 120 h after cell irradiation. Then the influence of PP(Ala)2(Arg)2- and PP(Arg)2-mediated PDT on early mitochondrial apoptosis induction via measurements of the transmembrane mitochondrial potential changes was examined. Results showed that lower energy doses and maximal nontoxic photosensitizer doses of PP(Ala)2(Arg)2 and PP(Arg)2 applied in PDT can imply apoptotic cell death. It was confirmed that modification of the protoporphyrin IX by attaching two alanine substituents raised the efficiency of photodynamic therapy.
    Źródło:
    Acta Biochimica Polonica; 2012, 59, 4; 603-611
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Orientation and spectral properties of two stilbazolium merocyanine dyes in stretched and unstretched polyvinyl alcohol films
    Autorzy:
    Dudkowiak, Alina
    Frąckowiak, Danuta
    Teślak, Ewa
    Gryczyński, Zygmunt
    Gryczyński, Ignacy
    Powiązania:
    https://bibliotekanauki.pl/articles/1041055.pdf
    Data publikacji:
    2007
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    anisotropic polymer matrix
    stilbazolium merocyanine dyes
    photodynamic therapy
    Opis:
    Spectral properties (anisotropy coefficients calculated for absorption, emission and fluorescence decay time) of two stilbazolium merocyanine dyes have been determined to evaluate the applicability of these dyes as sensitizers in photodynamic therapy. The dyes were embedded in an anisotropic polymer matrix. Analysis of the emission decay components measured in polarized light provides information on the interactions of the dye molecules with the polymer matrix being a model of an anisotropic biological system. Different values of the emission anisotropies obtained from various polarized components of fluorescence decays have shown that the orientations of the dye molecules influence their interactions with the polymer. This means that differently oriented dye molecules located in biological systems should exhibit different interactions with membranes. The chain length and type of side groups attached as well as the salt form of the dye molecule were shown to influence the dye-polymer interactions and should be taken into account before the application of merocyanine dyes in medicine. These dyes seem to be promising optical sensors with spectral properties, including the calculated anisotropy coefficients, sensitive to the molecular environment, useful to study orientation and interaction with neighbouring molecules in biological membranes.
    Źródło:
    Acta Biochimica Polonica; 2007, 54, 3; 647-656
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Spectral properties of phthalocyanines incorporated into resting and stimulated human peripheral blood cells.
    Autorzy:
    Waszkowiak, Aneta
    Frąckowiak, Danuta
    Wiktorowicz, Krzysztof
    Miyake, Jun
    Powiązania:
    https://bibliotekanauki.pl/articles/1043724.pdf
    Data publikacji:
    2002
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    phthalocyanines
    fluorescence
    photodynamic therapy
    human peripheral blood cells
    Opis:
    Human peripheral blood cells stimulated by phytohemagglutinin (which serve as a model of cancerous cells) and resting cells were incubated in dimethyl sulfoxide solutions of various phthalocyanines. In order to diminish the influence of atmospheric oxygen the cells were embedded in a polymer (polyvinyl alcohol) film. Fluorescence spectra of the samples were measured over two regions of excitation wavelengths: at 405 nm (predominant absorption of the cell material) and in the regions of strong absorption of phthalocyanines (at about 605 nm and 337 nm). The intrinsic emission of cell material became changed as a result both of cells' stimulation and of incubation of cells in dye solution. In most cases the stimulated cells when stained by dye exhibited higher long wavelength fluorescence intensity than resting cells. This suggests higher efficiency of dye incorporation into cancerous cells than into healthy cells. The absorption spectra of samples were also measured. The spectra of various phthalocyanines in incubation solvent, in polymer and in the cells embedded in polymer, were compared. The comparison of properties of the cells stimulated for different time periods enabled to establish the conditions of stimulation creating a population of cells incorporating a large number of sensitizing molecules.
    Źródło:
    Acta Biochimica Polonica; 2002, 49, 3; 633-641
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The interactions of phthalocyanines with stimulated and resting human peripheral blood mononuclear cells.
    Autorzy:
    Frąckowiak, Danuta
    Waszkowiak, Aneta
    Manikowski, Henryk
    Ion, Rodica-Mariana
    Cofta, Justyna
    Wiktorowicz, Krzysztof
    Powiązania:
    https://bibliotekanauki.pl/articles/1044196.pdf
    Data publikacji:
    2001
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    blood mononuclear cells
    photodynamic therapy
    interaction with phthalocyanines
    Opis:
    The interactions of two metal-free phthalocyanines [(H2Pc) and Solar Pc (with four peripherical groups: SO2N(CH2CH2OH)2)] and of one metal substituted dye (CoPc) with resting and stimulated human peripheral blood mononuclear cells (PBMC) were compared. The absorption, fluorescence, photoacoustic and EPR spectra of both resting cells and cells stimulated by phytohaemagglutinin, incubated in dimethyl sulfoxide (DMSO) with very low or 95% water content and with or without dye addition, were measured. The fate of the light absorbed by the samples was investigated. It is known that singlet oxygen production is crucial for photodynamic action of dyes. Thermal deactivation and luminescence emission compete with this process, so investigation of these alternative paths of sensitizer deactivation provides information about photodynamic action. The incorporation of the investigated dyes into cells and the perturbation of the cell structure caused by the dyes, the incubation solvent and the activator were investigated by comparing the spectral properties of PBMC before and after stimulation and incubation. Incubation of the cells for 1 h in a solution of Solar Pc in 99.5% aqueous DMSO, resulted in an efficient dye incorporation which was highly selective. Solar Pc being introduced much more efficiently into stimulated cells than into resting cells.
    Źródło:
    Acta Biochimica Polonica; 2001, 48, 1; 257-269
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Preliminary studies of phthalocyanine sensitizers incorporated into human leukemia cells from two cell-lines.
    Autorzy:
    Wiktorowicz, Krzysztof
    Cofta, Justyna
    Dudkowiak, Alina
    Waszkowiak, Aneta
    Frąckowiak, Danuta
    Powiązania:
    https://bibliotekanauki.pl/articles/1041548.pdf
    Data publikacji:
    2004
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    MOLT 4 cells
    fluorescence
    photodynamic therapy
    phthalocyanine
    CCRF cells
    Opis:
    Three phthalocyanine dyes-sensitizers were incorporated into two types of human T leukemia cells from two cell-lines: CCRF and MOLT 4. The efficiency of the dye incorporation into cells and photochemical properties of stained cells were investigated using fluorescence spectroscopy. The dyes exhibited different properties in each of the two cell-lines. Small differences in cell membrane properties have a strong influence on the efficiency of dye incorporation and on the course of photodynamic reaction. It is suggested that, for a given patient, an optimal dye-sensitizer should be established before photodynamic treatment.
    Źródło:
    Acta Biochimica Polonica; 2004, 51, 3; 703-710
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Pulmonary metastases of the A549-derived lung adenocarcinoma tumors growing in nude mice. A multiple case study
    Autorzy:
    Jakubowska, Monika
    Śniegocka, Martyna
    Podgórska, Ewa
    Michalczyk-Wetula, Dominika
    Urbanska, Krystyna
    Susz, Anna
    Fiedor, Leszek
    Pyka, Janusz
    Płonka, Przemysław
    Powiązania:
    https://bibliotekanauki.pl/articles/1039526.pdf
    Data publikacji:
    2013
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    adenocarcinoma
    histological analysis
    metastases
    nude mice
    photodynamic therapy
    zinc pheophorbide a
    Opis:
    Lung adenocarcinoma is a leading human malignancy with fatal prognosis. Ninety percent of the deaths, however, are caused by metastases. The model of subcutaneous tumor xenograft in nude mice was adopted to study the growth of control and photodynamically treated tumors derived from the human A549 lung adenocarcinoma cell line. As a side-result of the primary studies, observations on the metastasis of these tumors to the murine lungs were collected, and reported in the present paper. The metastasizing primary tumors were drained by a prominent number of lymphatic vessels. The metastatic tissue revealed the morphology of well-differentiated or trans-differentiated adenocarcinoma. Further histological and histochemical analyses demonstrated the presence of golden-brown granules in the metastatic tissue, similar to these found in the tumor tissue. In contrast to the primary tumors, the electron paramagnetic resonance spectroscopy revealed no nitric oxide - hemoglobin complexes (a source of intense paramagnetic signals), in the metastases. No metastases were found in other murine organs; however, white infarctions were identified in a single liver. Taken together, the A549-derived tumors growing subcutaneously in nude mice can metastasize and grow on site in the pulmonary tissue. Thus, they can represent an alternative for the model of induced metastatic nodule formation, following intravenous administration of the cancerous cells.
    Źródło:
    Acta Biochimica Polonica; 2013, 60, 3; 323-330
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Photodynamic effects of two water soluble porphyrins evaluated on human malignant melanoma cells in vitro.
    Autorzy:
    Szurko, Agnieszka
    Krämer-Marek, Gabriela
    Wideł, Maria
    Ratuszna, Alicja
    Habdas, Jan
    Kuś, Piotr
    Powiązania:
    https://bibliotekanauki.pl/articles/1043409.pdf
    Data publikacji:
    2003
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    malignant melanoma
    tumour megacolonies in vitro
    necrosis
    photodynamic therapy
    synthetic porphyrins
    apoptosis
    Opis:
    Two water soluble porphyrins: meso-tetra-4-N-methylpyridyl-porphyrin iodide (P1) and 5,10-di-(4-acetamidophenyl)-15,20-di-(4-N-methylpyridyl) porphyrin (P2) were synthesised and evaluated in respect to their photochemical and photophysical properties as well as biological activity. Cytotoxic and phototoxic effects were evaluated in human malignant melanoma Me45 line using clonogenic assay, cytological study of micronuclei, apoptosis and necrosis frequency and inhibition of growth of megacolonies. Both porphyrins were characterised by high UV and low visible light absorptions. Dark toxicity measured on the basis of the clonogenic assay and inhibition of megacolony growth area indicated that P1 was non-toxic at concentrations up to 50 μg/ml (42.14 μM) and P2 at concentrations up to 20 μg/ml (16.86 μM). The photodynamic effect induced by red light above 630 nm indicated that both porphyrins were able to inhibit growth of melanoma megacolonies at non-toxic concentrations. Cytologic examination showed that the predominant mode of cell death was necrosis.
    Źródło:
    Acta Biochimica Polonica; 2003, 50, 4; 1165-1174
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
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