- Tytuł:
- Overproduction and purification of the CcpA protein from Lactococcus lactis.
- Autorzy:
-
Kowalczyk, Magdalena
Bardowski, Jacek - Powiązania:
- https://bibliotekanauki.pl/articles/1043621.pdf
- Data publikacji:
- 2003
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
His-tag
cross links
dimers
CcpA - Opis:
- In this work we present cloning and overexpression of lactococcal CcpA protein in Escherichia coli Xl1blue strain as a fusion with 6×His tag. A high yield of the CcpA protein was obtained when the cells were cultured in liquid medium LB with 100 Μg/ml ampicillin at 37°C and subsequently for 4 h after induction by IPTG. The procedure let us obtain 5 mg of homogenous CcpA protein. Glutaraldehyde crosslinking analysis indicated the formation of dimer or tetramer forms of the CcpA protein.
- Źródło:
-
Acta Biochimica Polonica; 2003, 50, 2; 455-459
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł