- Tytuł:
- Interaction of maize (Zea mays) protein phosphatase 2A with tubulin
- Autorzy:
-
Awotunde, Olubunmi
Lechward, Katarzyna
Krajewska, Katarzyna
Żołnierowicz, Stanisław
Muszyńska, Grażyna - Powiązania:
- https://bibliotekanauki.pl/articles/1043655.pdf
- Data publikacji:
- 2003
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
protein phosphatase 2A
tubulin
protein-protein interaction
plant cytoskeleton organization
maize - Opis:
- Immunological and biochemical evidence has been obtained for an interaction of maize protein phosphatase 2A (PP2A) holoenzyme with tubulin. Tubulin co-purifies with maize seedling PP2A. Affinity chromatography of the maize PP2A preparation on immobilized tubulin revealed two peaks of phosphorylase a phosphatase activity. In one of the peaks, the catalytic (C) and constant regulatory (A) subunits of PP2A were identified by Western blotting. The subunits (C and A) of PP2A were co-immunoprecipitated from maize seedlings homogenate by an anti-α-tubulin antibody. The interaction of plant PP2A with tubulin indicates a possible role of reversible protein phosphorylation in the dynamic structure of plant cytoskeleton.
- Źródło:
-
Acta Biochimica Polonica; 2003, 50, 1; 131-138
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł