- Tytuł:
- Thiamine prevents X-ray induction of genetic changes in human lymphocytes in vitro.
- Autorzy:
-
Konopacka, Maria
Rogoliński, Jacek - Powiązania:
- https://bibliotekanauki.pl/articles/1041567.pdf
- Data publikacji:
- 2004
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
thiamine
X-radiation
necrosis
human lymphocytes
micronuclei
apoptosis - Opis:
- The effects of thiamine (vitamin B1) on the level of spontaneous or radiation-induced genetic changes in human lymphocytes in vitro were studied. Cultured lymphocytes were exposed to increasing concentrations of thiamine (0-500 μg/ml) and irradiated with X-rays. The DNA damage was estimated as the frequency of micronuclei and apoptotic or necrotic morphological changes in fixed cells. The results show that thiamine alone did not induce genetic changes. A significant decrease in the fraction of apoptotic and necrotic cells was observed in lymphocytes irradiated in the presence of vitamin B1 at concentrations between 1-100 μg/ml compared to those irradiated in the absence of thiamine. Vitamin B1 at 1 and 10 μg/ml decreased also the extent of radiation-induced formation of micronuclei. Vitamin B1 had no effect on radiation-induced cytotoxicity as measured by nuclear division index. The results indicate that vitamin B1 protects human cells from radiation-induced genetic changes.
- Źródło:
-
Acta Biochimica Polonica; 2004, 51, 3; 839-843
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł