- Tytuł:
- Type III CRISPR complexes from Thermus thermophilus
- Autorzy:
-
Szychowska, Marta
Siwek, Wojciech
Pawolski, Damian
Kazrani, Asgar
Pyrc, Krzysztof
Bochtler, Matthias - Powiązania:
- https://bibliotekanauki.pl/articles/1038832.pdf
- Data publikacji:
- 2016
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
CRISPR-Cas
endonuclease
Csm
Cmr
Thermus thermophilus
crRNA
mass spectrometry (MS)
chromatography - Opis:
- Pathogen-specific acquired immunity in bacteria is mediated by the CRISPR (clustered regularly interspaced short palindromic repeats)-Cas systems. Thermus thermophilus strain HB8 contains CRISPR systems of several major subtypes (type I, IIIA and IIIB), and has become a widely studied model for CRISPR biology. We have selected two highly expressed CRISPR spacers, crRNA 2.1 and crRNA 2.2, and have enriched endogenous T. thermophilus proteins that co-purify with these crRNAs. Mass spectroscopy indicates that the chromatography protocol enriches predominantly Csm complex subunits, but also Cmr subunits. After several chromatographic steps, size exclusion chromatography indicated a molecular mass of the crRNA associated complex of 265±69 kDa. In agreement with earlier work, crRNAs of different lengths (containing the selected spacers) were observed. Most of these were completely lost when several T. thermophilus csm genes were ablated.
- Źródło:
-
Acta Biochimica Polonica; 2016, 63, 2; 377-386
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł