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    Wyświetlanie 1-3 z 3
    Tytuł:
    Denaturation and aggregation of lysozyme in water-ethanol solution
    Autorzy:
    Szymańska, Agnieszka
    Hornowski, Tomasz
    Ślósarek, Genowefa
    Powiązania:
    https://bibliotekanauki.pl/articles/1039757.pdf
    Data publikacji:
    2012
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    volumetric studies
    monomer-to-filament structural transition
    lysozyme
    viscosity
    Opis:
    We have applied rheological methods for the analysis of ethanol-lysozyme interaction during the process of denaturation and aggregation of the protein. At low concentration of ethanol a destruction of the hydration shell of lysozyme is observed. With the increase in the ethanol concentration a structural transformation takes place. It leads to the formation of a protein aggregate with an elongated structure. The rheological characteristics of lysozyme-water-ethanol solution changes from Newtonian to pseudoplastic.
    Źródło:
    Acta Biochimica Polonica; 2012, 59, 2; 317-322
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Searching for anti-glioma activity. Ribonucleoside analogues with modifications in nucleobase and sugar moieties
    Autorzy:
    Framski, Grzegorz
    Wawrzyniak, Dariusz
    Jahnz-Wechmann, Zofia
    Szymanska-Michalak, Agnieszka
    Kraszewski, Adam
    Barciszewski, Jan
    Boryski, Jerzy
    Stawinski, Jacek
    Powiązania:
    https://bibliotekanauki.pl/articles/1038738.pdf
    Data publikacji:
    2016
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    ribonucleoside analogues
    anti-glioblastoma activity
    anticancer ribonucleotides
    Opis:
    Several ribonucleoside analogues with modifications in the nucleobase and sugar moiety have been screened for anti-glioma activity in the T98G glioma cell line using cervical (HeLa) cell line as reference human malignant cells, and lung fibroblast (MCR-5) cell line as non-cancerous reference cells. Among the investigated compounds, ribonucleosides containing 6-chloropurine (3), 7-guanine (5) and a pyrrolopyrimidine (18) as nucleobases, show promising anti-glioma activity with good selectivity indices, and can be considered as lead structures for further anti-cancer studies.
    Źródło:
    Acta Biochimica Polonica; 2016, 63, 4; 765-771
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Screening of lipase carriers for reactions in water, biphasic and pure organic solvent systems
    Autorzy:
    Hrydziuszko, Zofia
    Dmytryk, Agnieszka
    Majewska, Paulina
    Szymańska, Katarzyna
    Liesiene, Jolanta
    Jarzębski, Andrzej
    Bryjak, Jolanta
    Powiązania:
    https://bibliotekanauki.pl/articles/1039314.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    lipase
    immobilization
    hydrolysis
    transesterification
    Opis:
    In bioprocesses lipases are typically used in immobilized form, irrespective of type of reaction systems, to ensure an even distribution of catalysts in water restricted media and/or to facilitate separation and reuse. In these studies we report on the selection of appropriate enzyme-carrier preparation for hydrolysis reaction in aqueous and biphasic systems and transesterification in organic solvent. For this Candida rugosa lipase was bound by adsorption or covalent attachment onto various carriers to give 24 preparations. Selection of proper preparation was based on reactivity, thermal stability (4 h at 60°C), possibility of drying and operational stability in 17-23 successive batch processes of 4-nitrophenyl palmitate hydrolysis in water. Activity of preparations varied from 20 to 5100 U∙mL-1 but the most stable preparations were those of moderate activity: bound by adsorption or covalent attachment to NH2-Kieselgel or acrylic carrier (retained activity over 90%). Selected preparations were used for hydrolysis of ethyl (1-butyryloxyethyl)-phenylphosphinate in biphasic system, and, after drying, in ethyl (1-hydroxyethyl)-phenyl-phosphinate transesterification. In this study operational stability was the principal criterion of selection. In water system, lipase covalently bound to NH2-Kieselgel was the best - preserved 50% of initial activity in consecutive batch processes. In biphasic system and lipase covalently bound to acrylic and NH2-Kieselgel the values were 90 or 77%, respectively, whereas in organic solvent, when lipase was immobilized on NH2-Kieselgel by adsorption, it was 50%. Thus, NH2-Kieselgel appears to be an universal matrix for investigated lipase immobilization and can be used in all reaction systems.
    Źródło:
    Acta Biochimica Polonica; 2014, 61, 1; 1-6
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-3 z 3

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