Autor: Stępień, Agnieszka - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: A simple method for the determination of the cholesterol esterase activity
Autorzy:: Stępień, Agnieszka
Gonchar, Mykhailo
Powiązania:: https://bibliotekanauki.pl/articles/1039538.pdf
Data publikacji:: 2013
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: cholesterol esterase
cholesterol
activity determination
human serum
Opis:: The proposed method determines the activity of cholesterol esterase (CEH) and takes advantage of its ability to catalyze the hydrolysis of cholesterol esters naturally present in human serum. The assay is based on Allain's method of spectrophotometric determination of cholesterol by means of cholesterol oxidase, peroxidase, but using 3,5-dichloro-dihydroxybenzenesulfonic acid (DHBS) as phenolic chromogen and human serum as a source of substrate for the CEH as a novelty. Furthermore, it is characterized by low costs and high precision. It can be employed to control the activity of CE preparations used for the preparation of enzymatic kits for the determination of cholesterol or for screening of potential bacterial enzyme producers.
Źródło:: Acta Biochimica Polonica; 2013, 60, 3; 401-403
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Yeast nuclear PET127 gene can suppress deletions of the SUV3 or DSS1 genes: An indication of a functional interaction between 3' and 5' ends of mitochondrial mRNAs
Autorzy:: Węgierski, Tomasz
Dmochowska, Aleksandra
Jabłonowska, Agnieszka
Dziembowski, Andrzej
Bartnik, Ewa
Stępień, Piotr
Powiązania:: https://bibliotekanauki.pl/articles/1044752.pdf
Data publikacji:: 1998
Wydawca:: Polskie Towarzystwo Biochemiczne
Źródło:: Acta Biochimica Polonica; 1998, 45, 4; 935-940
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Human SUV3 helicase regulates growth rate of the HeLa cells and can localize in the nucleoli
Autorzy:: Szewczyk, Maciej
Fedoryszak-Kuśka, Natalia
Tkaczuk, Katarzyna
Dobrucki, Jurek
Waligórska, Agnieszka
Stępień, Piotr
Powiązania:: https://bibliotekanauki.pl/articles/1038704.pdf
Data publikacji:: 2017
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: SUV3 helicase
SUPV3L1
dual targeting
nucleolus
mitochondria
cell cycle
Opis:: The human SUV3 helicase (SUV3, hSUV3, SUPV3L1) is a DNA/RNA unwinding enzyme belonging to the class of DexH-box helicases. It localizes predominantly in the mitochondria, where it forms an RNA-degrading complex called mitochondrial degradosome with exonuclease PNP (polynucleotide phosphorylase). Association of this complex with the polyA polymerase can modulate mitochondrial polyA tails. Silencing of the SUV3 gene was shown to inhibit the cell cycle and to induce apoptosis in human cell lines. However, since small amounts of the SUV3 helicase were found in the cell nuclei, it was not clear whether the observed phenotypes of SUV3 depletion were of mitochondrial or nuclear origin. In order to answer this question we have designed gene constructs able to inhibit the SUV3 activity exclusively in the cell nuclei. The results indicate that the observed growth rate impairment upon SUV3 depletion is due to its nuclear function(s). Unexpectedly, overexpression of the nuclear-targeted wild-type copies of the SUV3 gene resulted in a higher growth rate. In addition, we demonstrate that the SUV3 helicase can be found in the HeLa cell nucleoli, but it is not detectable in the DNA-repair foci. Our results indicate that the nucleolar-associated human SUV3 protein is an important factor in regulation of the cell cycle.
Źródło:: Acta Biochimica Polonica; 2017, 64, 1; 177-181
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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