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    Wyświetlanie 1-6 z 6
    Tytuł:
    Molecular mechanisms of resistance to antifolates, a review
    Autorzy:
    Banerjee, Debabrata
    Ercikan-Abali, Emine
    Waltham, Mark
    Schnieders, Barbara
    Hochhauser, Daniel
    Li, Wei
    Fan, Jianguo
    Gorlick, Richard
    Goker, Erdem
    Bertino, Joseph
    Powiązania:
    https://bibliotekanauki.pl/articles/1045204.pdf
    Data publikacji:
    1995
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Źródło:
    Acta Biochimica Polonica; 1995, 42, 4; 457-464
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    A novel Δ12-fatty acid desaturase gene from methylotrophic yeast Pichia pastoris GS115
    Autorzy:
    Wei, D
    Li, M
    Zhang, X
    Zhou, H
    Xing, L
    Powiązania:
    https://bibliotekanauki.pl/articles/1041169.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    fatty acid desaturase gene
    Pichia pastoris
    Saccharomyces cerevisiae
    linoleic acid
    Opis:
    The methylotrophic yeast Pichia pastoris GS115, a widely used strain in production of various heterologous proteins, especially membrane-bound enzymes, can also produce linoleic and linolenic acids, which indicates the existence of membrane-bound Δ12 and Δ15-fatty acid desaturases. This paper describes the cloning and functional characterization of a novel Δ12-fatty acid desaturase gene from this methylotrophic yeast. The open reading frame of the gene (named Pp-FAD12) is 1263 bp in size and encodes a 420-amino-acid peptide. The deduced Pp-FAD12 protein shows high identity (50-67%) with Δ12-fatty acid desaturases from other fungi. It also shows a high identity (57%) with Δ15-fatty acid desaturase (named Sk-FAD15) from Saccharomyces kluyveri. Expression of Pp-FAD12 in polyunsaturated fatty acids non-producing yeast Saccharomyces cerevisiae demonstrated that its product converted oleic acid (18 : 1) to linoleic acid (18 : 2). This result suggests that Pp-FAD12 encodes a novel Δ12-fatty acid desaturase in P. pastoris GS115. This is the first report about the cloning and functional characterization of Δ12-fatty acid desaturase gene in methylotrophic yeast.
    Źródło:
    Acta Biochimica Polonica; 2006, 53, 4; 753-759
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Helicobacter pylori cytotoxin-associated gene A impairs the filtration barrier function of podocytes via p38 MAPK signaling pathway
    Autorzy:
    Yang, Man
    Wang, Ling
    Gu, Li-jie
    Yuan, Wei-jie
    Powiązania:
    https://bibliotekanauki.pl/articles/1038590.pdf
    Data publikacji:
    2017
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    CagA
    ZO-1
    p38 MAPK
    podocyte
    proteinuria
    Opis:
    Helicobacter pylori (Hp) specific antigens were found deposited in the glomeruli in some kidney diseases. However, the underlying molecular mechanisms remain to be elucidated. The aim of this study was to investigate the effect of cytotoxin associated gene A protein (CagA), a key virulence factor of Hp, on mouse podocytes. Cells were cultured and treated with recombinant CagA protein. The expression of the tight junction protein ZO-1 and p38 MAPK signaling pathway activation were measured with real-time RT-PCR and western blotting. The filtration barrier function of podocytes was evaluated with albumin influx assay. CagA decreased the expression and membrane distribution of ZO-1, impaired the filtration barrier function of podocytes, while activating p38 MAPK signaling pathway in these cells. Selective p38 MAPK inhibition partly prevented CagA-induced filtration barrier dysfunction of podocytes through ameliorating ZO-1 downregulation. Taken together, the results suggested that CagA, at least via p38 MAPK signaling pathway, may induce podocyte injury. Anti-Hp therapy may be beneficial for the treatment of kidney diseases related to Hp antigen deposition.
    Źródło:
    Acta Biochimica Polonica; 2017, 64, 3; 471-475
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Stigmasterol blocks cartilage degradation in rabbit model of osteoarthritis
    Autorzy:
    Chen, Wei-Ping
    Yu, Chong
    Hu, Peng-Fei
    Bao, Jia-Peng
    Tang, Jing-Li
    Wu, Li-Dong
    Powiązania:
    https://bibliotekanauki.pl/articles/1039645.pdf
    Data publikacji:
    2012
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    matrix metalloproteinases
    stigmasterol
    osteoarthritis
    Opis:
    Stigmasterol has been shown exihbit anti-osteoarthritic properties in vitro studies. However, the in vivo effects of stigmasterol on cartilage are still unclear. This study investigated the anti-osteoarthritic properties of stigmasterol on cartilage degradation in a rabbit model of osteoarthritis (OA). Twenty rabbits underwent bilateral anterior cruciate ligament transection (ACLT) to induce OA. Five rabbits were used as normal control. Two weeks after operation, the rabbits were randomly divided into two groups. Each group of 10 rabbits received intra-articular injection with 0.3 ml of stigmasterol in left knees and vehicle in right knees, once weekly. Group 1 was killed 6 weeks after ACLT and 2 were sacrificed 9 weeks after ACLT. The knee joints were assessed by gross morphology, histology and gene expression analysis. We found that expression of genes encoding matrix metalloproteinases (MMPs) was significantly higher while tissue inhibitors of metalloproteinase (TIMP)-1 was significantly lower in the both joints of the two OA groups compared to normal contrals. Stigmasterol reduced the cartilage degradation as assessed by histological analysis and markedly suppressed MMPs expression both in group 1 and group 2. Our results suggest that stigmasterol may be considered as a possible therapeutical agent in the treatment of OA.
    Źródło:
    Acta Biochimica Polonica; 2012, 59, 4; 537-541
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Two Gln187 mutants of human soluble APRIL inhibit proliferation of lung carcinoma A549 cells
    Autorzy:
    Dai, Shuangshuang
    Zheng, Yingru
    Chen, Bin
    Gao, Min
    Zhang, Yan
    Zhang, Li
    Gong, Wei
    He, Fengtain
    Powiązania:
    https://bibliotekanauki.pl/articles/1040492.pdf
    Data publikacji:
    2009
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    APRIL
    Gln187 mutant of sAPRIL
    anti-tumor activity
    Opis:
    Soluble APRIL (sAPRIL), the active form of a proliferation-inducing ligand (APRIL), is implicated in the proliferation of tumor cells. Suppressing APRIL function has been considered as a potential strategy for the therapy of APRIL-associated tumors. In the present study, we generated human sAPRIL and its two mutants, Gln187-D-sAPRIL (Gln187 deleted) and Gly187-sAPRIL (Gln187 replaced by Gly). In vitro experiments showed that the two mutants had similar specific binding capacity to lung carcinoma A549 cells compared to the wild-type sAPRIL, and both, especially Gly187-sAPRIL, exhibited significant antagonistic effect on sAPRIL-induced tumor cell proliferation in a dose-dependent manner, which might be predominantly mediated by blocking sAPRIL-induced MEK and ERK phosphorylation but not p38MAPK or JNK signaling. In vivo experiments with nude mice bearing A549 cell-derived xenograft tumor showed that only the Gly187-sAPRIL mutant could significantly suppress the tumor growth. These results suggest that Gln187 may be a crucial amino acid in APRIL-mediated tumor cell proliferation via the MEK-ERK signaling pathway and that the sAPRIL mutants may serve as novel potential antagonists of APRIL for the therapy of APRIL-associated cancers.
    Źródło:
    Acta Biochimica Polonica; 2009, 56, 4; 703-710
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Influence of oleic acid in different solvent media on BRL 3A cell growth and viability
    Autorzy:
    Liu, Runqi
    Yang, Wei
    Xia, Cheng
    Chen, Yuanyuan
    Gao, Sansi
    Dong, Zhihao
    Huang, Baoyin
    Li, Ruirui
    He, Ping
    Xu, Chuang
    Powiązania:
    https://bibliotekanauki.pl/articles/1038377.pdf
    Data publikacji:
    2018
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    oleic acid
    non-alcoholic fatty liver disease
    liver lipid deposition
    Opis:
    Oleic acid (OA) is widely used in pathology studies of hepatocellular lipid deposition. Identifying the effects of different solvents on OA-induced liver lipid deposition would be beneficial for studies on hepatocytes. We treated BRL 3A cells with OA dissolved in different solvents. After 12 h incubation, cell viability was assessed using MTT assays. Reactive oxygen species (ROS), triglyceride (TG) and total cholesterol (TC) counts, and the expression level of glucose regulated protein (GRP78), sterol regulatory element binding protein (SREBP-1C) and fatty acid synthase (FAS) were analyzed. Water, PBS and DMSO were disadvantageous to the dissolution of OA and did not cause an OA-induced response in hepatocytes. In the alcohol+OA-treated cells, the severe ER stress, oxidative stress and cellular fat deposition were significantly increased. BSA promoted cell growth and the cells treated with 1.2% BSA+OA showed a lower grade TG and endoplasmic reticulum stress compared with KOH+OA and alcohol+OA treatments. KOH had no significant influence on BRL 3A cells viability. When treated with OA dissolved in KOH, BRL 3A cells showed a typical hepatocyte damage. KOH was considered the suitable choice for an OA solvent for BRL 3A cells in hepatic lipidosis research.
    Źródło:
    Acta Biochimica Polonica; 2018, 65, 3; 443-447
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-6 z 6

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