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    Wyświetlanie 1-3 z 3
    Tytuł:
    Antibody response to DNA vaccine against H5N1 avian influenza virus in broilers immunized according to three schedules
    Autorzy:
    Stachyra, Anna
    Góra-Sochacka, Anna
    Zagórski-Ostoja, Włodzimierz
    Król, Ewelina
    Sirko, Agnieszka
    Powiązania:
    https://bibliotekanauki.pl/articles/1039267.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Avian influenza
    H5N1
    DNA vaccine
    broilers
    Opis:
    Broiler type chickens were immunized intramuscularly with a DNA vaccine encoding hemagglutinin (HA) from H5N1 avian influenza virus. The chickens were divided into four groups: control group which was not immunized, a group which obtained only one dose, and two groups which were immunized twice, one group with a boost two weeks after the priming and the other four weeks. Blood samples were collected at several time points and the dynamics of the humoral response to the vaccine was studied. High level of anti-HA antibodies was detected only in the last two groups, that is in chickens immunized according to the prime-boost strategy, regardless of the schedule. An additional interesting observation of this study was detection of the cross-reactivity of an anti-H5 HA positive serum with H5N2 and H1N1 viruses, suggesting that the DNA vaccine tested can induce antibodies of a broad specificity.
    Źródło:
    Acta Biochimica Polonica; 2014, 61, 3; 593-596
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Molecular characterization and symbiotic importance of prsD gene of Rhizobium leguminosarum bv. trifolii TA1
    Autorzy:
    Mazur, Andrzej
    Wielbo, Jerzy
    Król, Jarosław
    Kopcińska, Joanna
    Łotocka, Barbara
    Golinowski, Władysław
    Skorupska, Anna
    Powiązania:
    https://bibliotekanauki.pl/articles/1044765.pdf
    Data publikacji:
    1998
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Źródło:
    Acta Biochimica Polonica; 1998, 45, 4; 1067-1073
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Acute hepatologic and nephrologic effects of calcitriol in Syrian golden hamster (Mesocricetus auratus)
    Autorzy:
    Podgorska, Ewa
    Sniegocka, Martyna
    Mycinska, Marianna
    Trybus, Wojciech
    Trybus, Ewa
    Kopacz-Bednarska, Anna
    Wiechec, Olga
    Krzykawska-Serda, Martyna
    Elas, Martyna
    Krol, Teodora
    Urbanska, Krystyna
    Slominski, Andrzej
    Powiązania:
    https://bibliotekanauki.pl/articles/1038358.pdf
    Data publikacji:
    2018
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    calcitriol
    Syrian golden hamster
    hepatologic toxicity
    nephrologic toxicity
    Opis:
    Although vitamin D is included in the group of fat-soluble vitamins, it must be considered as a prohormone. Its active forms, including calcitriol, have pleiotropic effects and play an important role in the regulation of cell proliferation, differentiation and apoptosis, as well as in hormone secretion, and they demonstrate anti-cancer properties. Since calcitriol delivery can be beneficial for the organism, and Syrian golden hamsters represent a unique experimental model, we decided to investigate its toxicity in this species. In this study, we injected calcitriol intraperitoneally at doses 0 (control), 0.180±0.009 µg/kg and 0.717±0.032 µg/kg. Animal behavior was observed for 72 hrs after injection, and afterwards blood, liver and kidneys were collected for post-mortem examination, electron microscopy, and hematology analyses. The highest dose of calcitriol induced a change in animal behavior from calm to aggressive, and the liver surface showed morphological signs of damage. Following injection of calcitriol, ultrastructural changes were also observed in the liver and kidneys, e.g. vacuolization and increased number of mitochondria. There was also a trend for increased serum levels of aspartate aminotransferase (AST), but not of alanine aminotransferase (ALT) or GGTP (gamma-glutamyl transpeptidase). There was no change in Ca, Mg and P levels, as well as in blood morphology between experimental and control groups. These results indicate that calcitriol at 0.717, but not at 0.180 µg/kg, may induce acute damage to the liver and kidneys, without inducing calcemia. We propose that the hepatotoxic effect of calcitriol in hamster constitutes the primary cause of behavioral changes.
    Źródło:
    Acta Biochimica Polonica; 2018, 65, 3; 351-358
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-3 z 3

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