- Tytuł:
- Polarization angle dependence of stark absorption spectra of spirilloxanthin bound to the reconstituted LH1 complexes using LH1-subunits isolated from the purple photosynthetic bacterium Rhodospirillum rubrum
- Autorzy:
-
Horibe, Tomoko
Nakagawa, Katsunori
Kusumoto, Toshiyuki
Fujii, Ritsuko
Cogdell, Richard
Nango, Mamoru
Hashimoto, Hideki - Powiązania:
- https://bibliotekanauki.pl/articles/1039786.pdf
- Data publikacji:
- 2012
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
spirilloxanthin
Rhodospirillum rubrum
Stark absorption spectroscopy
polarization angle dependence
reconstitution
carotenoid
LH1 complex - Opis:
- Reconstituted LH1 complexes were prepared using the LH1 subunit-type complexes, isolated from the purple photosynthetic bacterium Rhodospirillum (Rs.) rubrum, and purified all-trans spirilloxanthin. Stark absorption spectra of spirilloxanthin bound to both the native and reconstituted LH1 complexes were compared in different polarization angles (χ) against the external electric field. From the polarization angle dependence of the Stark absorption spectra, two angles were determined in reference to the direction of transition dipole moment (m) of spirilloxanthin: one is the change in polarizability upon photoexcitation (Δα), θΔα and the other is the change in static dipole moment upon photoexcitation (Δμ), θΔμ. Despite the symmetric molecular structure of all-trans spirilloxanthin, its Stark absorption spectra show pronounced values of Δμ. This large Δμ values essentially caused by the effect of induced dipole moment through Δα both in the cases for native and reconstituted LH1 complexes. However, slightly different values of θΔα and θΔμ observed for the native LH1 complex suggest that spirilloxanthin is asymmetrically distorted when bound to the native LH1 complex and gives rise to intrinsic Δμ value.
- Źródło:
-
Acta Biochimica Polonica; 2012, 59, 1; 97-100
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł