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Wyszukujesz frazę "Chen, Cheng" wg kryterium: Autor


Wyświetlanie 1-3 z 3
Tytuł:
Influence of oleic acid in different solvent media on BRL 3A cell growth and viability
Autorzy:
Liu, Runqi
Yang, Wei
Xia, Cheng
Chen, Yuanyuan
Gao, Sansi
Dong, Zhihao
Huang, Baoyin
Li, Ruirui
He, Ping
Xu, Chuang
Powiązania:
https://bibliotekanauki.pl/articles/1038377.pdf
Data publikacji:
2018
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
oleic acid
non-alcoholic fatty liver disease
liver lipid deposition
Opis:
Oleic acid (OA) is widely used in pathology studies of hepatocellular lipid deposition. Identifying the effects of different solvents on OA-induced liver lipid deposition would be beneficial for studies on hepatocytes. We treated BRL 3A cells with OA dissolved in different solvents. After 12 h incubation, cell viability was assessed using MTT assays. Reactive oxygen species (ROS), triglyceride (TG) and total cholesterol (TC) counts, and the expression level of glucose regulated protein (GRP78), sterol regulatory element binding protein (SREBP-1C) and fatty acid synthase (FAS) were analyzed. Water, PBS and DMSO were disadvantageous to the dissolution of OA and did not cause an OA-induced response in hepatocytes. In the alcohol+OA-treated cells, the severe ER stress, oxidative stress and cellular fat deposition were significantly increased. BSA promoted cell growth and the cells treated with 1.2% BSA+OA showed a lower grade TG and endoplasmic reticulum stress compared with KOH+OA and alcohol+OA treatments. KOH had no significant influence on BRL 3A cells viability. When treated with OA dissolved in KOH, BRL 3A cells showed a typical hepatocyte damage. KOH was considered the suitable choice for an OA solvent for BRL 3A cells in hepatic lipidosis research.
Źródło:
Acta Biochimica Polonica; 2018, 65, 3; 443-447
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Ezetimibe prevents myocardial remodeling in an obese rat model by inhibiting inflammation
Autorzy:
Li, Xiao-Xing
Zhao, Lang
Chang, Ying
Liu, Bao-Shan
Xu, Feng
Zhang, Cheng
Ji, Xiao-Ping
Chen, Yu-Guo
Li, Chuan-Bao
Powiązania:
https://bibliotekanauki.pl/articles/1038380.pdf
Data publikacji:
2018
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
obese
inflammation
remodeling
ezetimibe
IL-6
Opis:
Inflammation plays an important role in the development of many obesity-related diseases. This study aimed to investigate the effect of ezetimibe on inflammation and myocardial remodeling in obese rats. A rat model of obesity was established, and myocardial damage was examined by transmission electron microscopy and Masson staining. Twenty obese rats were divided into two groups (n=10): obese group and ezetimibe group. Ten SD rats were used as controls. Western blot was performed to monitor the expression of P-p38MAPK and interleukin (IL)-6. Immunohistochemical staining was used to monitor the expression of intercellular adhesion molecule-1 and vascular cell adhesion molecule-1. In the obese rats group, we observed increased inflammatory factors and myocardial hypertrophy. In contrast, the ezetimibe group exhibited decreased expression of inflammatory factors and an improvement in myocardial remodeling compared to the obese group. Mechanistically, we found that ezetimibe decreased P-p38MAPK, IL-6, intercellular adhesion molecule-1, and vascular cell adhesion molecule-1 levels in the hearts of the obese rats. Taken together, these results indicate that ezetimibe may improve myocardial remodeling in obese rats by inhibiting inflammation.
Źródło:
Acta Biochimica Polonica; 2018, 65, 3; 465-470
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Myocardial remodeling in rats with metabolic syndrome: role of Rho-kinase mediated insulin resistance
Autorzy:
Li, Chuan-Bao
Li, Xiao-Xing
Chen, Yu-Guo
Gao, Hai-Qing
Bao, Cheng-Mei
Liu, Xiang-Qun
Bu, Pei-Li
Zhang, Juan
Zhang, Yun
Ji, Xiao-Ping
Powiązania:
https://bibliotekanauki.pl/articles/1039743.pdf
Data publikacji:
2012
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Rat
Metabolism
Myocardium
Phosphorylation
Insulin resistance
Opis:
Insulin resistance (IR) plays a critical role in metabolic syndrome (MS). Previous studies have demonstrated that activated ROCK is increased in MS patients. However, the effect of Rho-kinase (ROCK) on IR has not been definitely determined. Thus, the aims of the present study were to determine whether ROCK activation induces IR or affects myocardial structure and function, as well as the possible mechanisms underlying this process. Wistar rats fed high fat, high glucose and high salt diet sewed as model of MS and we used transmission electron microscopy, echocardiogram technology, and terminal deoxynucleotidyl transferase-mediated DNA nick-end labeling staining to identify any myocardial damage. The protein levels of MYPT-1 (characteristic of ROCK activation), IRS-1 and AKT were analyzed by immunohistochemistry and Western blotting. In hearts from MS rats, we found increased protein levels of phospho-MYPT-1 and phospho-IRS-1 (Ser307) and decreased phospho-AKT compared to levels in normal rats. In conclusion, the results suggest that ROCK-mediated IR is involved in the development of myocardial impairments in MS rats and that this effect is mediated probably via the IRS-1/PI3-kinase/AKT pathway.
Źródło:
Acta Biochimica Polonica; 2012, 59, 2; 249-254
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-3 z 3

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