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    Wyświetlanie 1-4 z 4
    Tytuł:
    Sampling, metadata and DNA extraction - important steps in metagenomic studies
    Autorzy:
    Felczykowska, Agnieszka
    Krajewska, Anna
    Zielińska, Sylwia
    Łoś, Joanna
    Powiązania:
    https://bibliotekanauki.pl/articles/1039153.pdf
    Data publikacji:
    2015
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    sampling
    DNA extraction
    DNA purification
    soil
    water
    wastewater
    metadata
    Opis:
    Metagenomic studies have become increasingly popular. They allow for the estimation of biodiversity in complex populations. This diversity presents an enormous but largely unexpected genetic and biological pool and can be exploited for the recovery of novel genes, entire metabolic pathways and their products. Generally metagenomic study is a genomic analysis of organisms by direct extraction and cloning of DNA from their natural environment. The most common problems of modern metagenomics are as follows: majority of the microorganisms present in the environment cannot be cultivated by standard techniques, DNA extraction methods are not very effective, isolated DNA is contaminated with various compounds, a choice for a screening method is not obvious.
    Źródło:
    Acta Biochimica Polonica; 2015, 62, 1; 151-160
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    First insight into microbial community composition in a phosphogypsum waste heap soil
    Autorzy:
    Zielińska, Sylwia
    Radkowski, Piotr
    Ossowski, Tadeusz
    Ludwig-Gałęzowska, Agnieszka
    Łoś, Joanna
    Łoś, Marcin
    Powiązania:
    https://bibliotekanauki.pl/articles/1038561.pdf
    Data publikacji:
    2017
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    microbial community
    16S rRNA gene
    soil sample
    postproduction waste
    phosphogypsum
    reclamation
    Opis:
    The aim of this study was to investigate the soil microbial communities of a phosphogypsum waste heap. The soil microbial community structures can differ over time, as they are affected by the changing environmental conditions caused by a long-term exposure to different kinds of pollutions, like is the case of soil in the post-production waste area in Wiślinka (in the northern part of Poland) currently undergoing restoration. Our analyses indicated that the most abundant phyla were Proteobacteria, Acidobacteria, and Actinobacteria, and generally such an abundance is common for most of the studied soils. The most dominant class were Alphaproteobacteria, with their participation in 33.46% of the total reads. Among this class, the most numbered order was Sphingomonadales, whereas among this order the Sphingomonadaceae family was the most abundant one. The Sphingomonadaceae family is currently in the center of interest of many researchers, due to the ability of some of its members to utilize a wide range of naturally occurring organic compounds and many types of environmental contaminants. This kind of knowledge about microbial populations can support efforts in bioremediation and can improve monitoring changes in the contaminated environments.
    Źródło:
    Acta Biochimica Polonica; 2017, 64, 4; 693-698
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The most widespread problems in the function-based microbial metagenomics
    Autorzy:
    Felczykowska, Agnieszka
    Krajewska, Anna
    Zielińska, Sylwia
    Łoś, Joanna
    Bloch, Sylwia
    Nejman-Faleńczyk, Bożena
    Powiązania:
    https://bibliotekanauki.pl/articles/1039154.pdf
    Data publikacji:
    2015
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    function-based metagenomics
    metagenomic library screening
    gene expression
    microbials
    databases
    codon usage bias
    cell accumulation
    Opis:
    Metagenomics is a powerful tool to better understand the microbial niches, especially these from extreme habitats like oceans and seas, hot springs or deserts. However, one who is going to face the metagenomic studies should realize the challenges which might occur in the course of experiments. This manuscript indicates common problems in function-driven metagenomics, especially factors that influence gene expression are taken into account. Codon usage bias, internal cell accumulation, correct protein folding or presence of proper initiation factors are discussed and possible ways to overcome these problems are proposed. Finally, the annotation process is described, including possible limitations that one should take under consideration. What is more, the most popular databases for metagenomic data are mentioned and discussed.
    Źródło:
    Acta Biochimica Polonica; 2015, 62, 1; 161-166
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Influence of acetaminophen and trichloroethylene on liver cytochrome P450-dependent monooxygenase system.
    Autorzy:
    Plewka, Andrzej
    Zielińska-Psuja, Barbara
    Kowalówka-Zawieja, Joanna
    Nowaczyk-Dura, Grażyna
    Plewka, Danuta
    Wiaderkiewicz, Anna
    Kamiński, Marcin
    Orłowski, Jerzy
    Powiązania:
    https://bibliotekanauki.pl/articles/1044264.pdf
    Data publikacji:
    2000
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    trichloroethylene
    liver
    rat
    acetaminophen
    cytochrome P450,glutathione
    Opis:
    The aim of the study was to evaluate the effect of acetaminophen (APAP) and/or trichloroethylene (TRI) on the liver cytochrome P450-dependent monooxygenase system, CYP2E1 and CYP1A2 (two important P450 isoforms), and liver glutathione (GSH) content in rats. Rats were given three different doses of APAP (250, 500 and 1000 mg/kg b...) and then the above-mentioned parameters were measured for 48 h. The lowest APAP dose produced small changes in the cytochrome P450 content of liver. At 500 mg/kg APAP increased the cytochrome P450 content to 230% of the control. The inductive effect was seen at 1000 mg/kg dose but at 24 h and later. NADPH-cytochrome P450 reductase activity was the highest after the lowest dose of APAP, while after the highest dose it was equal to the control value. TRI increased both the cytochrome P450 content and the NADPH-cytochrome P450 reductase activity. When TRI was combined with APAP, both these parameters increased in the first hours of observation, but they returned to the control values at 24 h. When APAP was given at 250 mg/kg, GSH levels decreased to 55% of the control at 8 h and returned to the control values at 24 h. The higher doses of APAP decreased GSH levels more than the lowest dose, but after 24 h GSH levels did not differ from those of the control. When TRI was given at 250 mg/kg, the GSH levels decreased to 68% of the control at 2 h and then they increased gradually and tended to exceed the control values at 48 h. The effect of TRI combined with APAP on the level of GSH was virtually the same as that of APAP alone given at 500 mg/kg.
    Źródło:
    Acta Biochimica Polonica; 2000, 47, 4; 1129-1136
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-4 z 4

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