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Wyświetlanie 1-4 z 4
Tytuł:
Effect of different wavelengths of light on laccase, cellobiose dehydrogenase, and proteases produced by Cerrena unicolor, Pycnoporus sanguineus and Phlebia lindtneri
Autorzy:
Janusz, Grzegorz
Sulej, Justyna
Jaszek, Magdalena
Osińska-Jaroszuk, Monika
Powiązania:
https://bibliotekanauki.pl/articles/1038799.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
white rot fungi
submerged cultures
visible light wavelenghts
Opis:
Three species of white rot fungi: Cerrena unicolor, Phlebia lindtneri and Pycnoporus sanguineus were cultured in two different media under five different lighting conditions: dark, white, red, blue, and green light. Laccase, cellobiose dehydrogenase, and protease activities were examined in the samples. Blue light efficiently boosted laccase synthesis in C. unicolor and P. sanguineus, whereas the highest activities (20 654 nkat/l) of P. lindtneri laccase were observed when this fungus was maintained in green light. On the contrary, the green light allowed obtaining the highest activities of cellobiose dehydrogenase of C. unicolor and P. lindtneri, while CDH of P. sanguineus seems to be dependent on white light. It is clearly visible that differences in protease activities are noticeable not only between the lights variants but also among the media used. However, high proteases activities are correlated with light variants inducing laccase in Lindeberg and Holm medium. Contrary to the cellulose-based medium, where they are weak in light variants that lead to high CDH activities.
Źródło:
Acta Biochimica Polonica; 2016, 63, 2; 223-228
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Determination of biodiversity of Coprinus comatus using genotyping and metabolic profiling tools
Autorzy:
Pawlik, Anna
Malinowska, Anna
Siwulski, Marek
Frąc, Magdalena
Rogalski, Jerzy
Janusz, Grzegorz
Powiązania:
https://bibliotekanauki.pl/articles/1038885.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
AFLP
ITS
Biolog
fungal diversity
Coprinus comatus
shaggy mane mushroom
Opis:
Coprinus comatus strains (CCMs) originating from Poland were identified using ITS region sequencing. Based on the sequences obtained, the genetic relationship between the CCM strains was determined and a clear separation of all strains into two main clusters was obtained. The Coprinus strains were also genetically characterized for the first time by the AFLP technique. The analysis showed that the CCMs separated into four main clusters and a high complication of a UPGMA-based dendrogram was achieved. C. comatus strains included in the analysis displayed an AFLP profile similarity level in the range from 44 to 66%. The highest similarity coefficient, 0.490, was found between CCM12 and CCM13, and the lowest (0.202) between the CCM2 and CCM5 isolates. Biolog FF MicroPlates were applied to obtain data on utilization of 95 carbon sources and mycelial growth. The analysis allowed comparison of the functional diversity of the CCM strains and revealed a broad variability within the analyzed Coprinus species based on substrate utilization profiles. Significant differences (2-48) have been shown in the substrate richness values. The Biolog experiments proved to be a good profiling technology for studying the diversity in shaggy manes due to metabolic differences and demonstrated that all the strains might be considered individually. It is evident that the strain metabolic grouping does not correlate with the grouping based on the ITS sequences and AFLP profiles, however, some similarities may be observed.
Źródło:
Acta Biochimica Polonica; 2015, 62, 4; 683-689
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The phenotypic and genomic diversity of Aspergillus strains producing glucose dehydrogenase
Autorzy:
Rola, Beata
Pawlik, Anna
Frąc, Magdalena
Małek, Wanda
Targoński, Zdzisław
Rogalski, Jerzy
Janusz, Grzegorz
Powiązania:
https://bibliotekanauki.pl/articles/1038906.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Aspergillus
glucose dehydrogenase
identification
metabolic diversity
Opis:
Twelve Aspergillus sp. strains producing glucose dehydrogenase were identified using ITS region sequencing. Based on the sequences obtained, the genomic relationship of the analyzed strains was investigated. Moreover, partial gdh gene sequences were determined and aligned. The amplified fragment length polymorphism (AFLP) method was applied for genomic fingerprinting of twelve Aspergillus isolates. Using one PstI restriction endonuclease and five selective primers in an AFLP assay, 556 DNA fragments were generated, including 532 polymorphic bands. The AFLP profiles were found to be highly specific for each strain and they unambiguously distinguished twelve Aspergilli fungi. The AFLP-based dendrogram generated by the UPGMA method grouped all the Aspergillus fungi studied into two major clusters. All the Aspergillus strains were also characterized using Biolog FF MicroPlates to obtain data on C-substrate utilization and mitochondrial activity. The ability to decompose various substrates differed among the analyzed strains up to three folds. All of the studied strains mainly decomposed carbohydrates.
Źródło:
Acta Biochimica Polonica; 2015, 62, 4; 747-755
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Diversity of influenza-like illness etiology in Polish Armed Forces in influenza epidemic season
Autorzy:
Kocik, Janusz
Niemcewicz, Marcin
Winnicka, Izabela
Michalski, Aleksander
Bielawska-Drózd, Agata
Kołodziej, Marcin
Joniec, Justyna
Cieślik, Piotr
Graniak, Grzegorz
Mirski, Tomasz
Gaweł, Jerzy
Bielecka-Oder, Anna
Kubiak, Leszek
Russell, Kevin
Powiązania:
https://bibliotekanauki.pl/articles/1039252.pdf
Data publikacji:
2014
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Influenza
ILI
surveillance
Polish Army
Opis:
The aim of this study was to conduct an epidemiological and laboratory surveillance of Influenza-Like Illnesses (ILI) in Polish Armed Forces, civilian military personnel and their families in 2011/2012 epidemic season, under the United States Department of Defense-Global Emerging Infections Surveillance and Response System (DoD-GEIS). ILI incidence data were analyzed in relation to age, gender, patient category as well as pathogen patterns. Multiple viral, bacterial and viral-bacterial co-infections were identified. Nose and throat swabs of active duty soldiers in the homeland country and in the NATO peacekeeping forces KFOR (Kosovo Force), as well as members of their families were tested for the presence of viral and bacterial pathogens. From October 2011 to May 2012, 416 specimens from ILI symptoms patients were collected and analyzed for the presence of viral and bacterial pathogens. Among viruses, coronavirus was the most commonly detected. In the case of bacterial infections, the most common pathogen was Staphylococcus aureus.
Źródło:
Acta Biochimica Polonica; 2014, 61, 3; 489-494
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-4 z 4

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