- Tytuł:
- Benzo[a]pyrene and cyclopenta[c]phenanthrene suppress expression of p53 in head kidney of rainbow trout
- Autorzy:
-
Brzuzan, P.
Woźny, M.
Góra, M.
Łuczyński, M. K.
Jabłońska, A. - Powiązania:
- https://bibliotekanauki.pl/articles/363082.pdf
- Data publikacji:
- 2011
- Wydawca:
- Uniwersytet Warmińsko-Mazurski w Olsztynie
- Tematy:
-
B[a]P
CP[c]Ph
ekspresja genów
hnRNA
pstrąg tęczowy
poziom transkrypcji
gene expression
rainbow trout
transcription rate - Opis:
- Although p53, a protein of important tumor suppressive function, has been extensively studied in mammals, relatively little is known about the p53 pathways in lower vertebrates. Particularly, limited information exists on possible influences of environmental contaminants on the expression of the p53 gene in fish. In the current study, we assessed the effects of benzo[a]pyrene (B[a]P; potent tumor promoter) and cyclopenta[c]phenanthrene (CP[c]Ph; clastogenic agent) exposure on a 24h profile of p53 gene expression in head kidney of juvenile rainbow trout (Oncorhynchus mykiss). To analyze the p53 transcription rate, we developed protocol for the examination of both mRNA and heterogeneous nuclear (hn) RNA of the gene, using Real-Time RTPCR approach. The results show that both compounds are capable of suppressing p53 transcriptional activity within 12h of the treatment. Our finding supports the idea that structurally different PAHs may influence cell physiologic functions controlled by p53 in fish, in part, by down-regulating its RNA expression levels.
- Źródło:
-
Environmental Biotechnology; 2011, 7, 1; 41-45
1734-4964 - Pojawia się w:
- Environmental Biotechnology
- Dostawca treści:
- Biblioteka Nauki
Artykuł