Temat: Bombyx mori cells - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Pinellia ternata agglutinin produced in Bombyx mori cells exhibits bioactivity
Autorzy:: Xu, Tao
Wang, Bo
Wang, Liya
Zhang, Yaozhou
Lv, Zhengbing
Powiązania:: https://bibliotekanauki.pl/articles/1039738.pdf
Data publikacji:: 2012
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: Pinellia ternata agglutinin
silkworm bioreactor
plant lectin
Bombyx mori cells
Opis:: Pinellia ternata agglutinin (PTA) is highly homologous to many other monocot mannose-binding lectins which reportedly possess antitumor activities. Its production in silkworm cells has great application potential because the baculovirus expression system can produce post-translationally modified proteins at low cost. In the current study, the pta gene was cloned and expressed in silkworm cells, and the expressed protein was analyzed using a hemagglutination assay. A preliminary in vitro study on its anti-proliferative activity was performed. The results show that the recombinant PTA with an apparent molecular mass of 29 kDa can hemagglutinate rabbit erythrocytes and this activity can be inhibited by D-mannan at a low concentration. In addition, the recombinant hemagglutinin exhibited a dose-dependent anti-proliferative activity on hepatoma cells. The results of the current study suggest that PTA and other important bioactive proteins could be produced by silkworm bioreactor for biomedicine research and application.
Źródło:: Acta Biochimica Polonica; 2012, 59, 2; 231-236
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Knitted silk mesh-like scaffold incorporated with sponge-like regenerated silk fibroin/collagen I and seeded with mesenchymal stem cells for repairing Achilles tendon in rabbits
Autorzy:: Tang, L.
Yang, Y.
Li, Y.
Yang, G.
Luo, T.
Xue, Y.
Zhang, W.
Powiązania:: https://bibliotekanauki.pl/articles/306523.pdf
Data publikacji:: 2018
Wydawca:: Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:: ścięgno Achillesa
kolagen
komórki macierzyste
szpik kostny
Bombyx mori silk
regenerated silk fibroin
collagen I
bone marrow-derived mesenchymal stem cells
weft-knit
Achilles tendon action
Opis:: A scaffold knit with natural sericin-free silk fibroin fiber possesses desirable mechanical properties, biocompatibility, ease of fabrication, and slow degradability. However, regenerated silk fibroin degrades faster than natural silk. In this study, natural silk fibroin fiber mesh-like scaffolds were prepared by a weft-knitting method and the pores were filled with sponge-like regenerated silk fibroin-collagen I. The microporous sponge and mesh-like scaffolds were fused to achieve gradient degradation of the scaffolds, and rabbit bone marrow mesenchymal stem cells (BMSCs) were seeded onto the scaffolds to form scaffold–BMSCs composites. The composites were implanted into gap defects made in the rabbit Achilles tendon. Twenty weeks after implantation, histological observation showed that tendon-like tissue had formed, collagen I mRNA was expressed, abundant collagen was generated, and that there was no obvious degradation of silk. The maximum load of the neo-Achilles tendon was 62.14% that of the natural Achilles tendon. These outcomes were superior to those obtained in the group implanted with a scaffold without BMSCs. These findings suggest the feasibility of constructing tissue-engineered tendons using weft-knitted silk scaffolds incorporated with sponge-like regenerated silk fibroin/collagen I and seeded with BMSCs, and show potential of the scaffold–BMSCs composites to repair Achilles tendon defects.
Źródło:: Acta of Bioengineering and Biomechanics; 2018, 20, 3; 77-87
1509-409X
2450-6303
Pojawia się w:: Acta of Bioengineering and Biomechanics
Dostawca treści:: Biblioteka Nauki

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