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    Wyświetlanie 1-10 z 10
    Tytuł:
    Fragments of LINE-1 retrotransposons flanked by inverted telomeric repeats are present in the bovine genome. Homology with human LINE-1 elements
    Autorzy:
    Płucienniczak, Grażyna
    Płucienniczak, Andrzej
    Powiązania:
    https://bibliotekanauki.pl/articles/1044443.pdf
    Data publikacji:
    1999
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    L1MC4
    LINE-1
    retrotransposons
    telomeric repeats
    Opis:
    In the bovine genome we found two intrachromosomal DNA fragments flanked by inverted telomeric repeats (GenBank Accession Nos. AF136741 and AF136742). The internal parts of the fragments are homologous exclusively to the human sequences and to the consensus sequence of the L1MC4 subfamily of LINE-1 retrotransposons which are widespread among mammalian genomes. We found that distribution of homologous human sequences within our fragments is not random, reflecting a complicated pattern of insertion mechanisms of and maintenance of retrotransposons in mammalian genomes. One of the possible explanations of the origin of LINE-1 truncated elements flanked by inverted telomeric repeats in the bovine genome is that extrachromosomal DNA fragments may be modified by telomerase and subsequently, transferred into chromosomal DNA.
    Źródło:
    Acta Biochimica Polonica; 1999, 46, 4; 873-878
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Integrony
    Integrons
    Autorzy:
    Wolinowska, Renata
    Masny, Aleksander
    Płucienniczak, Andrzej
    Powiązania:
    https://bibliotekanauki.pl/articles/1201713.pdf
    Data publikacji:
    2002
    Wydawca:
    Polskie Towarzystwo Przyrodników im. Kopernika
    Opis:
    Accumulation of antibiotic resistance among pathogenic bacteria has called attention to horizontal gene transfer that involves plasmids and transpozons. Integrons, usually placed on mobile genome elements, are very deeply engaged in the process of origin of multiple-drug-resistant strains. Integrons are genetic elements that contain determinants of the components of the site-specific recombination system that recognizes and captures mobile gene cassettes. More than 70 different antibiotic resistance genes covering most classes of antimicrobials presently in use have been detected in gene cassettes. Integrons are frequently found in clinical and environmental strains of gram-negative rods. The discovery of super-integrons, i.e. genetic structures gathering gene cassettes in a huge number, led to the conception of genome cassettes capture as an element of a broader phenomenon of bacterial genome modification in response to changing environmental conditions.
    Źródło:
    Kosmos; 2002, 51, 3; 353-364
    0023-4249
    Pojawia się w:
    Kosmos
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The factor VIII protein and its function
    Autorzy:
    Mazurkiewicz-Pisarek, Anna
    Płucienniczak, Grażyna
    Ciach, Tomasz
    Płucienniczak, Andrzej
    Powiązania:
    https://bibliotekanauki.pl/articles/1038835.pdf
    Data publikacji:
    2016
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Factor VIII (FVIII)
    molecular structure
    function
    hemophilia A
    bleeding disorders
    Opis:
    Factor VIII (FVIII), an essential blood coagulation protein, is a key component of the fluid phase blood coagulation system. Human factor VIII is a single chain of about 300 kDa consisting of domains described as A1-A2-B-A3-C1-C2. The protein undergoes processing prior to secretion into blood resulting in a heavy chain of 200 kDa (A1-A2-B) and a light chain of 80 kDa (A3-C1-C2) linked by metal ions. The role of factor VIII is to increase the catalytic efficiency of factor IXa in the activation of factor X. Variants of these factors lead frequently also to severe bleeding disorders.
    Źródło:
    Acta Biochimica Polonica; 2016, 63, 1; 11-16
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Adaptation of PCR technique for quantitative estimation of genetic material from different regions of chromosome 21 in cases of trisomy 21.
    Autorzy:
    Nowacka, Joanna
    Helszer, Zofia
    Walter, Zofia
    Płucienniczak, Andrzej
    Kałużewski, Bogdan
    Powiązania:
    https://bibliotekanauki.pl/articles/1041513.pdf
    Data publikacji:
    2004
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    quantitative polymerase chain reaction
    trisomy 21
    Opis:
    Pre- and postnatal diagnosis of chromosomal aberrations is generally based on conventional cytogenetic analysis. In this paper, we have devised a quantitative polymerase chain reaction (Q-PCR) method to determine gene dose effects and applied it in cases of regular trisomy 21 as a model. The method is based on quantitative assessment of PCR products after using primers amplifying DNA fragments located in the pericentromeric, heterochromatic, euchromatic and telomeric regions of chromosome 21. A gene dose effect on the amount of PCR product in cases of trisomy 21 was confirmed. Moreover, a correlation between the amount of the PCR product of the examined sequences and their location in the chromosome was observed. The obtained results suggest that the Q-PCR technique can be applied in the diagnosis of aneuploidies.
    Źródło:
    Acta Biochimica Polonica; 2004, 51, 4; 995-1001
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Expression of bovine leukemia virus protein p24 in Escherichia coli and its use in the immunoblotting assay.
    Autorzy:
    Bicka, Leokadia
    Kuźmak, Jacek
    Kozaczyńska, Bożena
    Płucienniczak, Andrzej
    Skorupska, Anna
    Powiązania:
    https://bibliotekanauki.pl/articles/1044191.pdf
    Data publikacji:
    2001
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    bovine leukemia virus
    fusion protein p24
    immunoblotting assay
    gag gene cloning
    Opis:
    The gag gene encoded protein, p24 of bovine leukemia virus (BLV), was cloned and expressed as thioredoxin-6xHis-p24 protein in Escherichia coli. The bacterial cells carrying plasmid pT7THis-p24 expressed the protein of 38 kDa that was detected by immunoblotting analysis using anti-p24 monoclonal antibodies and sera from BLV infected cattle and sheep. The purified p24 fusion protein was shown to be sensitive and specific for detection of BLV antibodies in the infected cattle.
    Źródło:
    Acta Biochimica Polonica; 2001, 48, 1; 227-232
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    In vitro phosphorylation of ex-preprotachykinin by protein kinase C
    Autorzy:
    Hrabec, Elżbieta
    Hrabec, Zbigniew
    Lachowicz, Lilla
    Greger, Janusz
    Płucienniczak, Grażyna
    Płucienniczak, Andrzej
    Powiązania:
    https://bibliotekanauki.pl/articles/1045392.pdf
    Data publikacji:
    1994
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Źródło:
    Acta Biochimica Polonica; 1994, 41, 2; 194-195
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The effect of Arg209 to Lys mutation in mouse thymidylate synthase.
    Autorzy:
    Cieśla, Joanna
    Gołos, Barbara
    Wałajtys-Rode, Elżbieta
    Jagielska, Elżbieta
    Płucienniczak, Andrzej
    Rode, Wojciech
    Powiązania:
    https://bibliotekanauki.pl/articles/1043728.pdf
    Data publikacji:
    2002
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    nucleotide's phosphate binding
    thymidylate synthase
    mutation
    Opis:
    Mouse thymidylate synthase R209K (a mutation corresponding to R218K in Lactobacillus casei), overexpressed in thymidylate synthase-deficient Escherichia coli strain, was poorly soluble and with only feeble enzyme activity. The mutated protein, incubated with FdUMP and N5,10-methylenetetrahydrofolate, did not form a complex stable under conditions of SDS/polyacrylamide gel electrophoresis. The reaction catalyzed by the R209K enzyme (studied in a crude extract), compared to that catalyzed by purified wild-type recombinant mouse thymidylate synthase, showed the Km value for dUMP 571-fold higher and Vmax value over 50-fold (assuming that the mutated enzyme constituted 20% of total crude extract protein) lower. Thus the ratios kcat, R209K/kcat, 'wild' and (kcat, R209K/Km, R209KdUMP)/( kcat, 'wild'/Km, 'wild'dUMP) were 0.019 and 0.000032, respectively, documenting that mouse thymidylate synthase R209, similar to the corresponding L. casei R218, is essential for both dUMP binding and enzyme reaction.
    Źródło:
    Acta Biochimica Polonica; 2002, 49, 3; 651-658
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Molecular basis of cellulose biosynthesis disappearance in submerged culture of Acetobacter xylinum
    Autorzy:
    Krystynowicz, Alina
    Koziołkiewicz, Maria
    Wiktorowska-Jezierska, Agnieszka
    Bielecki, Stanisław
    Klemenska, Emilia
    Masny, Aleksander
    Płucienniczak, Andrzej
    Powiązania:
    https://bibliotekanauki.pl/articles/1041378.pdf
    Data publikacji:
    2005
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    2-D electrophoresis
    UDP-glucose pyrophosphorylase
    phosphoglucomutase
    bacterial cellulose
    Acetobacter xylinum
    PCR-MP
    Opis:
    Acetobacter xylinum strains are known as very efficient producers of bacterial cellulose which, due to its unique properties, has great application potential. One of the most important problems faced during cellulose synthesis by these bacteria is generation of cellulose non-producing cells, which can appear under submerged culture conditions. The reasons of this remain unknow. These studies have been undertaken to compare at the molecular level wild-type, cellulose producing (Cel+) A. xylinum strains with Cel- forms of cellulose-negative phenotype. Comparison of protein profiles of both forms of A. xylinum by 2D electrophoresis allowed for the isolation of proteins which were produced exclusively by either Cel+ or Cel- cells. Sequences of peptides derived from these proteins were aligned with those of proteins deposited in databases. This analysis revealed that Cel- cells lacked two enzymes: phosphoglucomutase and glucose-1-phosphate uridylyltransferase, which generates UDP-glucose being the substrate for cellulose synthase. DNA was analyzed by ligation-mediated PCR carried out at low denaturation temperature (PCR-MP). Two DNA fragments of different thermal stability (218 and 217 bp) were obtained from the DNA of Cel+ and Cel- forms, respectively. The only difference between these Cel- and Cel+ DNA fragments is deletion of one T residue. Alignment of those two sequences with those deposited in the GenBank database revealed that similar fragments are present in the genomes of some bacterial cellulose producers and are located downstream from open reading frames (ORF) encoding phosphoglucomutase. The meaning of this observation is discussed.
    Źródło:
    Acta Biochimica Polonica; 2005, 52, 3; 691-698
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The epidermal growth factor-like domain from tissue plasminogen activator. Cloning in E. coli, purification and ESR studies of its interaction with human blood platelets
    Autorzy:
    Pietrucha, Tadeusz
    Stec, Wojciech
    Okruszek, Andrzej
    Uznański, Bogdan
    Koziołkiewicz, Maria
    Wilk, Andrzej
    Płucienniczak, Andrzej
    Świątkowska, Maria
    Cierniewski, Czesław
    Powiązania:
    https://bibliotekanauki.pl/articles/1045415.pdf
    Data publikacji:
    1994
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Źródło:
    Acta Biochimica Polonica; 1994, 41, 1; 25-34
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-10 z 10

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