- Tytuł:
- Adhesion and growth of vascular cells on porous polyethylene terephthalate scaffolds
- Autorzy:
-
Havlikova, J.
Turek, K.
Dajko, G.
Bacakova, L. - Powiązania:
- https://bibliotekanauki.pl/articles/283781.pdf
- Data publikacji:
- 2010
- Wydawca:
- Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
- Tematy:
-
polymers
scaffolds
vascular cells - Opis:
- Polymers such as polyethylene terephthalate (PET) have been used for large-caliber vascular prostheses with a relative success but their application is limited in small-caliber grafts. Blood vessel grafts with an internal diameter smaller than 6 mm are prone to fail mainly due to their thrombogenicity and poor haemodynamics. One of the possible solutions of these problems may be reconstruction of the tunica intima and media on the synthetic grafts. For this purpose, special PET foils were prepared. Six-μm thick foils were irradiated by copper ions or fission fragments from a radionuclide etalon source 252Cf and etched by 1M sodium hydroxide to obtain holes of a defined diameter (from 80 to100 nm in foils irradiated by copper ions and from 1.0 to 1.5 μm in foils irradiated by fission fragments) and density (1x106 cm-2 - fission fragments to 5x108 cm-2 – copper ions) (FIG.1). Afterward these materials were seeded with vascular smooth muscle cells (VSMC) derived from the rat aorta, or endothelial cells of the line CPAE. Adhesion, proliferation and viability of the cells were monitored after one, three and seven days. The cell proliferation was evaluated by changes in the cell number in several time intervals and construction of growth curves. Determination of cell viability was based on staining of live cells with calcein emitting green fluorescence, and the dead cells with ethidium bromide emitting red fluorescence. Experiments with the growth of vascular smooth muscle cells and endothelial cells on the PET scaffolds with different pore size showed that endothelial cells prefer pores around 1 μm while VSMC have no preferences concerning the pore size of the polymer scaffolds tested. Although the highest cell population densities were found on the glass coverslips used as control material, the number of cells growing on pristine PET did not differ from the densities on PET foils irradiated by Cu-ions or fission fragments of Cf. The obtained data showed applicability of our improved polymer foils as supporting scaffolds for vascular cells. In the further step, these porous PET membranes could serve as synthetic analogues of internal elastic lamina separating vascular smooth muscle cells and endothelial cells in a newly constructed bioartificial vascular wall.
- Źródło:
-
Engineering of Biomaterials; 2010, 13, no. 99-101; 108-109
1429-7248 - Pojawia się w:
- Engineering of Biomaterials
- Dostawca treści:
- Biblioteka Nauki
Artykuł