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Tytuł:
A study to determine the most effective daymark shape for a leading line
Autorzy:
Ahmad Fuad, A F.
Adlan, D. A. A.
Kamis, A. S.
Ahmad, A. S.
Powiązania:
https://bibliotekanauki.pl/articles/2211922.pdf
Data publikacji:
2022
Wydawca:
Uniwersytet Morski w Gdyni. Wydział Nawigacyjny
Tematy:
navigational infractructure
leading line
daymark
daymark shape
navigational infrastructure
Port of Kemaman
leading line daymark
IALA recommendations
Opis:
The use of daymark shapes for leading lines include its visual shape and symbol, to aid sailors who need to navigate safely through a marked narrow channel. The shapes which are currently used are rectangles, diamonds, and triangles. However, there is not much information on which shape is the most effective for navigational referencing. Therefore, the main objective of this research is to determine the most effective shape for a leading line daymark. The Kemaman Port was used as an area of interest to model the three-dimensional simulation. The size and height of the leading line daymark was calculated based on the dimensions of the navigational channel obtained from the Kemaman Port navigation chart. The simulation for different shapes of the leading mark in the same area was developed using a three-dimensional software. Experts were engaged to evaluate the effectiveness of each daymark shape across the maximum and minimum distances during the simulation run. Results showed that the diamond shape daymark was relatively better identified and used as reference, compared to other shapes in the same range due to its relatively larger surface area. Therefore, the study objective was achieved, and the results can be used to improve marine navigation safety.
Źródło:
TransNav : International Journal on Marine Navigation and Safety of Sea Transportation; 2022, 16, 3; 497--500
2083-6473
2083-6481
Pojawia się w:
TransNav : International Journal on Marine Navigation and Safety of Sea Transportation
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A comparison study between advance transfer technique and advance transfer mathematical model using bulk carrier ship: cross-track distance validation by percentage change and mann whitney u test
Autorzy:
Kamis, A. S.
Ahmad Fuad, A. S.
Powiązania:
https://bibliotekanauki.pl/articles/2211981.pdf
Data publikacji:
2022
Wydawca:
Uniwersytet Morski w Gdyni. Wydział Nawigacyjny
Tematy:
bulk carriers
coss-track distance
ECDIS
wheel over point
advanced transfer technique
advance transfer mathematical model
wilcoxon-mann whitney u tes
IBM SPSS
Opis:
One of the methods of efficient course alteration is through the accurate identification of the WOP by ATT. ATT is widely used by mariners worldwide, and recently, the technique has been restructured and enhanced into ATMM. To prove the efficacy of ATMM over ATT, a few types of ships have been used to carry out the manoeuvring analysis. This study extends the analysis by using a bulk carrier ship. A ship simulator was used for a manoeuvring simulation study, which was carried out to verify the differences between these two methods. Throughout the manoeuvring simulation study, XTD data for each simulation was monitored and verified by XTL compliance, percentage variation, and the Wilcoxon-Mann Whitney U Test via IBM SPSS. It was found that the ATMM can produce a significantly improved WOP compared to ATT and is suitable to be used onboard a bulk carrier ship. This research's finding is expected to contribute as evidence to strengthen ATMM's efficiency so that it can be accepted as an ECDIS algorithm for ship navigation.
Źródło:
TransNav : International Journal on Marine Navigation and Safety of Sea Transportation; 2022, 16, 3; 541--551
2083-6473
2083-6481
Pojawia się w:
TransNav : International Journal on Marine Navigation and Safety of Sea Transportation
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Parametric optimization of rice bran oil extraction using response surface methodology
Autorzy:
Ahmad, S. W.
Javed, F.
Ahmad, S.
Akram, M.
Rehman, A.
Powiązania:
https://bibliotekanauki.pl/articles/778979.pdf
Data publikacji:
2016
Wydawca:
Zachodniopomorski Uniwersytet Technologiczny w Szczecinie. Wydawnictwo Uczelniane ZUT w Szczecinie
Tematy:
solvent extraction
rice bran oil
response surface methodology (RSM)
central composite design (CCD)
Opis:
Use of bran oil in various edible and nonedible industries is very common. In this research work, efficient and optimized methodology for the recovery of rice bran oil has been investigated. The present statistical study includes parametric optimization, based on experimental results of rice bran oil extraction. In this study, three solvents, acetone, ethanol and solvent mixture (SM) [acetone: ethanol (1:1 v/v)] were employed in extraction investigations. Response surface methodology (RSM), an optimization technique, was exploited for this purpose. A five level central composite design (CCD) consisting four operating parameter, like temperature, stirring rate, solvent-bran ratio and contact time were examined to optimize rice bran oil extraction. Experimental results showed that oil recovery can be enhanced from 71% to 82% when temperature, solvent-bran ratio, stirring rate and contact time were kept at 55°C, 6:1, 180 rpm and 45 minutes, respectively while fixing the pH of the mixture at 7.1.
Źródło:
Polish Journal of Chemical Technology; 2016, 18, 3; 103-109
1509-8117
1899-4741
Pojawia się w:
Polish Journal of Chemical Technology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A concept explanation on the development of wheel over point mathematical model for efficient course alteration
Autorzy:
Kamis, A. S.
Ahmad Fuad, A. F.
Powiązania:
https://bibliotekanauki.pl/articles/2063988.pdf
Data publikacji:
2021
Wydawca:
Uniwersytet Morski w Gdyni. Wydział Nawigacyjny
Tematy:
wheel over point
IBM SPSS
ECDIS
efficient course alteration
mathematical model
altering of the course
Mann-Whitney U Test
Opis:
This concept paper described the process of bridging the gaps in one of the methods for determining wheel over point (WOP). WOP is a marking made on charted courses to indicate a point at which a ship should change course. Identifying WOP is vital to avoid the vessel overshooting the planned track. One method for determining WOP is the advanced transfer technique. However, two issues were identified while reviewing this technique. Therefore, an improved mathematical model could be developed to overcome these problems. A manoeuvring analysis using a ship simulator will be performed to test the developed mathematical model's efficacy. The data obtained from the simulation study can be validated further by its adherence to the cross track limit, reduction in percentage change, and using IBM SPSS for the Mann-Whitney U test. The developed mathematical model is expectantly capable of producing a better track-keeping function and suitable for use onboard a cargo ship. The mathematical model also could be implemented as an algorithm in the Electronic Chart Display and Information System to help navigators make more efficient course changes.
Źródło:
TransNav : International Journal on Marine Navigation and Safety of Sea Transportation; 2021, 15, 3; 795--798
2083-6473
2083-6481
Pojawia się w:
TransNav : International Journal on Marine Navigation and Safety of Sea Transportation
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Ethnobotanical investigation of wild vegetables used among local communities in northwest Pakistan
Autorzy:
Ahmad, K.
Weckerle, C.S.
Nazir, A.
Powiązania:
https://bibliotekanauki.pl/articles/57306.pdf
Data publikacji:
2019
Wydawca:
Polskie Towarzystwo Botaniczne
Opis:
Wild vegetables can contribute to people’s food security and health. In northwest Pakistan, almost 40% of the households are classified as food insecure, due to increasing population and natural and man-made catastrophes. There is an urgent need to get an overview of still existing practices of wild vegetable use and to incorporate such knowledge into agricultural policies. The present study, therefore, aims to collect and analyze information on the most widely and commonly used wild vegetables in northwest Pakistan. Semistructured interviews were performed with 126 informants (26–78-year old) from all 24 districts of the Khyber Pakhtunkhwa Province. Information on culinary and medicinal use, cooking methods, and growing and harvest season were collected. A rating scale was used to get the opinion on quality, abundance, and use frequency of wild vegetables. Information on trading was gathered on local and regional markets. Transect walks were done with key informants for specimen collection. A total of 25 wild vegetable species (21 genera, 13 families) were documented. Most of them are herbs (22 spp.). One third of the species needs processing or detoxification prior to cooking. Taste and food quality as perceived by the local people are the main driver for use frequency and commercialization. Length of availability varies from 2 to 7 months with Rumex dentatus and Vicia faba also available during the winter season. Overall, 21 spp. are also used medicinally, mainly for gastrointestinal diseases. Leaves are the preferred plant part for both vegetables and medicines. Nine species are sold in markets, Bauhinia variegata and Caralluma tuberculata throughout the whole province. Local communities in northwest Pakistan have a broad knowledge of local plant use, especially on wild vegetables and their adequate processing. The present study suggests that the most commonly used wild vegetables should be promoted to reinforce food safety and most of them may also be commercialized. Conservation priority has to be given to the commercially harvested Caralluma tuberculata, which is presently overexploited.
Źródło:
Acta Societatis Botanicorum Poloniae; 2019, 88, 1
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Phosphate-induced changes in fatty acid biosynthesis in Chlorella sp. KS-MA2 strain
Autorzy:
Ahmad, A.
Osman, S.M.
Cha, T.S.
Loh, S.H.
Powiązania:
https://bibliotekanauki.pl/articles/80623.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2016, 97, 4
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Foliar application of salicylic acid with salinity stress on physiological and biochemical attributes of sunflower (Helianthus annuus L.) crop
Autorzy:
Noreen, S.
Siddiq, A.
Hussain, K.
Ahmad, S.
Hasanuzzama, M.
Powiązania:
https://bibliotekanauki.pl/articles/11543526.pdf
Data publikacji:
2017
Wydawca:
Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Opis:
The potential agricultural lands are falling prey to salinity in the world over including Pakistan. The limited water supply is also becoming a serious problem to feed the humans and livestock production. Therefore, research studies were undertaken to enhance the growth and development of sunflower (Helianthus annuus L.) on saline soils to increase productively of crop. The treatments consisted of (a) two lines of sunflower (Hysun-33 and LG-56-63), (b) two levels of salinity (0, 120 mM (NaCl)) and (c) two levels of salicylic acid (0, 200 mg L-1) and were arranged in a completely randomized design with four replications. The results showed that biological yield was significantly reduced due to imposition of salinity at the rate of 120 mM (NaCl) on both sunflower lines (Hysun -33, LG 56-63). The stem length was also reduced due to decrease in biological yield in response to salinity. However, the exogenous application of salicylic acid at the rate of 200 mg L-1 mitigated the adverse effects of salts and improved the biological yield and stem length under saline and non-saline environments. The quantity of chlorophyll (SPAD) values were impacted negatively in response to salt stress, however, the phenomenon was recovered by foliar spray of salicylic acid. The nutrient concentration of K+, Cl- and Na+ were altered because of presence of excess quantity of NaCl in the substrate. The translocation of K+ ion was reduced substantially, while higher amount of Na+ and Cl- ions were absorbed, thus creating ionic imbalance in the plant system. The foliar spray of salicylic acid (200 mg L-1) enhanced the uptake of K+ from the soil medium. The salicylic acid proved a potential phytoprotectant to mitigate the adverse effects of salinity and thereby improving the physiological and biochemicals attributes, stem length and also enhanced uptake of K+ ion while depressing Na+ and Cl- ions in plant system.
Źródło:
Acta Scientiarum Polonorum. Hortorum Cultus; 2017, 16, 2; 57-74
1644-0692
Pojawia się w:
Acta Scientiarum Polonorum. Hortorum Cultus
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Success factors of project and process management — lessons learned from EPPM 2016
Autorzy:
Ahmad, H. S.
Bazlamit, S. M.
Jurczuk, A.
Orłowski, C.
Powiązania:
https://bibliotekanauki.pl/articles/125625.pdf
Data publikacji:
2017
Wydawca:
Politechnika Białostocka. Oficyna Wydawnicza Politechniki Białostockiej
Tematy:
post-conference report
materiał pokonferencyjny
Opis:
The aim of this post-conference report is to present key-issues delivered by participants of the Project and Process Management sessions (PPM) at the 7th International Conference on Engineering, Project, and Production Management (EPPM 2016). EPPM 2016 was held in Bialystok, Poland in 21-23 September. The format of the EPPM 2016 Conference involved a mixture of keynote speeches, individual presentations on topical issues, and extensive panel discussions. It featured 89 papers in five thematic sessions across the two conference days. Over 115 delegates attended EPPM 2016 from more than 20 countries across Europe, Asia, and the rest of the world.
Źródło:
Engineering Management in Production and Services; 2017, 9, 3; 7-9
2543-6597
2543-912X
Pojawia się w:
Engineering Management in Production and Services
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Dominant factors affecting the behavior of innovative employees
Główne czynniki wpływające na innowacyjne zachowania pracowników
Autorzy:
Fauzia, S.
Budiningsih, I.
Djaelani, A.
Ahmad, M.
Powiązania:
https://bibliotekanauki.pl/articles/404829.pdf
Data publikacji:
2017
Wydawca:
Politechnika Częstochowska
Tematy:
zachowanie innowacyjne
organizacja ucząca się
dzielenie się wiedzą
organizacja
zaangażowanie
innovative behavior
learning organization
knowledge sharing
organizational
commitment
Opis:
The aim of the studies is to determine the model of the relationship between innovative employee behavior with learning organization, knowledge sharing, and organizational commitment. The method of the research is survey in 166 employees selected randomly at Multicentral Aryaguna Company - Jakarta, and analyzed by using descriptive, correlation and regression. The study fine that learning organization, knowledge sharing and organizational commitment collectively contribute to enhancinginnovative employee behavior in 62% and 38% of other factors, its mean learning organization, knowledge sharing, and organizational commitment cannot be ignored’ as an instrument to improve innovative employee behavior.
Celem badań jest określenie modelu relacji pomiędzy innowacyjnymi zachowaniami pracowników, a organizacją uczącą się i dzieleniem się wiedzą. Badanie wykazało, że organizacja ucząca się, dzielenie się wiedzą oraz zaangażowanie organizacyjne wspólnie przyczyniają się do zwiększenia innowacyjnych zachowań pracowników w 62% i 38% innych czynników, co oznacza, że organizacja ucząca się, dzielenie się wiedzą i zaangażowanie organizacyjne, jako instrumenty poprawy innowacyjnego zachowania pracownika, nie mogą być ignorowane. Metodą badań była ankieta przeprowadzona wśród 166 wybranych losowo pracowników przedsiębiorstwa Multicentral Aryaguna w Dżakarcie i przeanalizowana za pomocą opisu, korelacji i regresji.
Źródło:
Polish Journal of Management Studies; 2017, 16, 1; 32-40
2081-7452
Pojawia się w:
Polish Journal of Management Studies
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Prediction cost maintenance model of office building based on condition-based maintenance
Predykcyjno-kosztowy model konserwacji budynku biurowego oparty o utrzymanie zależne od bieżącego stanu technicznego (CBM)
Autorzy:
Au-Yong, C. P.
Ali, A. S.
Ahmad, F.
Powiązania:
https://bibliotekanauki.pl/articles/301654.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Polskie Naukowo-Techniczne Towarzystwo Eksploatacyjne PAN
Tematy:
charakterystyki
utrzymanie zależne od bieżącego stanu technicznego (CBM)
wydajność kosztu
budynek biurowy
Malezja
characteristics
condition-based maintenance
cost performance
office building
Malaysia
Opis:
Koszty konserwacji budynków nieustannie rosną ze względu na nieodpowiednią konserwację. Z tej racji, niezbędne jest wypracowanie rozwiązań obniżających koszty konserwacji. Różne badania wykazały, iż charakterystyki utrzymania urządzeń zależnie od ich bieżącego stanu technicznego (condition-based maintenance, CBM) są bezpośrednio powiązane z wydajnością kosztu. Niniejszy artykuł stara się więc ustalić związek pomiędzy charakterystykami utrzymania budynków zależnie od ich bieżącego stanu technicznego a wydajnością kosztu. Następnie opracowano model regresji dla planowania konserwacji jak i predykcji. W badaniach użyto metody mieszanej łączącej badania kwestionariuszowe, wywiad oraz studium przypadku. Rezultaty podkreśliły, iż wiarygodność danych z konserwacji i informacji to najbardziej istotne charakterystyki CBM. W konsekwencji, wnioski z badań sugerują, iż planowanie i wdrożenie strategii utrzymania w zależności od bieżącego stanu technicznego powinno brać pod uwagę jej istotne charakterystyki i odwoływać się do wynikającego z niej modelu predykcji. Ponadto, praca zawiera zalecenia jakimi środkami można w praktyce poprawić istotne charakterystyki i wydajność kosztu.
Building maintenance costs are continuously increasing as a result of poor maintenance. Consequently, there is an urgent need to develop solutions to reduce the maintenance costs. Various studies demonstrated that the characteristics of condition-based maintenance are directly related to the cost performance. Thus, this paper seeks to establish the relationships between the characteristics of condition-based maintenance and the cost performance. The researcher then developed a regression model for maintenance planning and prediction. The study adopted a mix method approach that includes questionnaire survey, interview, and case study. The findings highlighted the reliability of maintenance data and information as the most significant characteristic of conditionbased maintenance. Consequently, the study concluded that the planning and the application of the condition-based maintenance strategy should consider its significant characteristics and make reference to the resulting prediction model. Furthermore, the study recommended measures to improve the significant characteristics and the cost performance in practice.
Źródło:
Eksploatacja i Niezawodność; 2014, 16, 2; 319-324
1507-2711
Pojawia się w:
Eksploatacja i Niezawodność
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Impact of family-based selection on growth performance and immune response of Japanese quail
Autorzy:
Rehman, A.
Hussain, J.
Mahmud, A.
Javed, K.
Ghayas, A.
Ahmad, S.
Powiązania:
https://bibliotekanauki.pl/articles/2087135.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
weight base selection
egg base selection
growth performance
immune response
Źródło:
Polish Journal of Veterinary Sciences; 2021, 24, 4; 479-486
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Indication of urban expansion of Gujranwala city (Pakistan) by remote sensing and GIS
Autorzy:
Aziz, A.
Anwar, M.M.
Rani, M.
Ahmad, S.
Zaheer, S.
Powiązania:
https://bibliotekanauki.pl/articles/2049753.pdf
Data publikacji:
2019
Wydawca:
Fundacja na Rzecz Młodych Naukowców
Tematy:
remote sensing
industrial revolution
Earth surface system
human influence
ecological process
human activity
temporal variation
GIS
urban sprawl
ecology
time
Opis:
Land-cover by land-use shows the influence of human on earth surface by different phenomena’s, ecological processes and human activities, its indication is a parameter of measurement how and with which extent the human influenced the natural surface with temporal variations. The change detection technique is used based upon the temporal observations from 2000 to 2015. Study area is one of the most populated city of Pakistan where industrial revolution is at its peak. The whole study area is categorized into four classes (i.e. Agricultural, Barren, Built-up and semi natural surfaces). The observational intervals were divided into three main classes i.e. 2000 to 2005, 2005 to 2010 and 2010 to 2015. Remarkable changes were occurred in built up area from 2000 to 2015 which is 38.6 % of total area, while the total change/decreased in barren land is -332 9(ha), similarly the Parks and grasses are also in decreasing extent and affect the local biodiversity and natural environment. The land cover is directly proportional to population expansion of urban city. For making the prediction of future urban expansion, previously occurred changes were aggregated to come up with a single value and then adding it to the recent land-use values. The mapping and analysis was undertaken through techniques of remote sensing and GIS, which proved to be very effective to measure the changes, and trend of changes with time, this mapping not only tells us the scale of change but, also tells us the patterns and the forces which causes these changes.
Źródło:
Environment, Earth and Ecology; 2019, 3, 1; 13-23
2543-9774
2451-4225
Pojawia się w:
Environment, Earth and Ecology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Impact of membrane pore structure on protein detection sensitivity of affinity-based immunoassay
Autorzy:
Ahmad, A. L.
Ideris, N.
Ooi, B.S.
Low, S.C.
Ismail, A.
Powiązania:
https://bibliotekanauki.pl/articles/777927.pdf
Data publikacji:
2016
Wydawca:
Zachodniopomorski Uniwersytet Technologiczny w Szczecinie. Wydawnictwo Uczelniane ZUT w Szczecinie
Tematy:
morphology
PVDF membrane
protein immobilization
Opis:
Understanding a membrane’s morphology is important for controlling its final performance during protein immobilization. Porous, symmetric membranes were prepared from a polyvinylidene fluoride/N-methyl-2-pyrrolidinone solution by phase inversion process, to obtain membrane with various microsized pores. The concentration and surface area of aprotein dotted on the membrane surface were measured by staining with Ponceau S dye. The dotted protein was further scanned and analysed to perform quantitative measurements for relative comparison. The intensity of the red protein spot and its surface area varied depending on the membrane pore size, demonstrating the dependence of protein immobilization on this factor. The membrane with the smallest pore size (M3) showed the highest protein spot intensity and surface area when examined at different protein concentrations. An increase in the applied protein volume showed a linearity proportional trend to the total surface area, and an uneven round dot shape was observed at a large applied volume of protein solution.
Źródło:
Polish Journal of Chemical Technology; 2016, 18, 2; 97-103
1509-8117
1899-4741
Pojawia się w:
Polish Journal of Chemical Technology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Detection and copy number estimation of the transgenic nucleotide sequences in an unknown GM event of Oryza sativa
Detekcja i oszacowanie liczby kopii transgenu cry1Ac kodującego cechę odporności na szkodniki w genetycznie modyfikowanym ryżu (Oryza sativa)
Autorzy:
Sajjad, A.M.
Bashir, T.
Saeed, S.
Ahmad, E.
Powiązania:
https://bibliotekanauki.pl/articles/28252.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Botaniczne
Opis:
The present study was designed to establish a qualitative detection method based on conventional and real time PCR assay to screen the commonly grown rice varieties for the presence of the cry1Ac gene. The detection of genetically modified rice in the screening process would necessitate accurate assay development and precise qualitative PCR tests complying with established procedures for the detection and characterization of transgenes in food grains. Such assay would not only enable the monitoring of transgene flow in local agricultural environment but also the characterization of different plant species produced with this transgene and its regulatory components. Thus, a reliable and quick screening assay was established for the qualitative detection of the transgene along with the promoter and selectable marker gene in genetically modified rice. By conventional PCR, a fragment of 215 bp was amplified with gene specific primers of cry1Ac. Primers for other transgenes such as gna and bar were also employed; however, no amplification was detected. The presence of the p35s, sps, and nptII genes was confirmed by qualitative real-time PCR. The specificity of the respective PCR products was checked through melt peak curve analysis. Sharp and precise melting temperatures indicated the presence of a single kind of PCR product in correspondence to each of the primers used. Moreover, the copy number of cry1Ac was estimated by ΔΔCT method. It is proposed that the primer sets and experimental conditions used in this study will be sufficient to meet the requirements for molecular detection and characterization of the cry1Ac transgene and affiliated sequences in sorting out conventional rice varieties from the ones which are genetically modified. It will also help to monitor the ecological flow of these transgenes and other biosafety factors.
Celem niniejszych badań było opracowanie metody detekcji genu cry1Ac, odpowiedzialnego za odporność na szkodniki, z wykorzystaniem konwencjonalnej techniki PCR oraz PCR w czasie rzeczywistym, w celu badania obecności tego transgenu w powszechnie uprawianych odmianach ryżu. Detekcja modyfikacji genetycznej obecnej w odmianach ryżu wymaga opracowania metodyki badawczej z wykorzystaniem metody PCR, zgodnie z ustalonymi procedurami dotyczącymi obecności transgenów w zbożach konsumpcyjnych. Badania te nie tylko umożliwiłyby monitorowanie przepływu transgenów w lokalnym środowisku rolnym, ale również dokonanie charakterystyki różnych gatunków roślin wytworzonych z wykorzystaniem oznaczanego transgenu oraz związanych z nim fragmentów regulatorowych. W niniejszej pracy przedstawiono metodę wiarygodnego testu kontrolnego, w celu detekcji transgenu wraz z promotorem i selekcyjnym genem markerowym. Przy użyciu konwencjonalnej techniki PCR z zastosowaniem starterów specyficznych dla genu cry1Ac powielono fragment długości 215 pz. Poszukiwano także, lecz nie wykryto, innych transgenów, takich jak gna i bar. Obecność genów p35s, sps i nptII została potwierdzona na podstawie techniki ilościowego PCR w czasie rzeczywistym. Poszczególne produkty PCR poddano analizie z wykorzystaniem krzywych topnienia. Ostre wierzchołki krzywych topnienia wskazywały na obecność pojedynczego produktu PCR, odpowiednio dla każdego użytego startera. Za pomocą metody ΔΔCT oszacowano liczbę kopii genu cry1Ac. Na podstawie niniejszych badań wykazano, że zestawy starterów i opracowana metodyka są właściwe do molekularnej detekcji i charakterystyki transgenu cry1Ac oraz sekwencji z nim związanych i mogą służyć do wskazania genetycznie modyfikowanych odmian ryżu. Metoda ta może być również pomocna w monitorowaniu środowiska pod względem przepływu tego transgenu.
Źródło:
Acta Agrobotanica; 2016, 69, 4
0065-0951
2300-357X
Pojawia się w:
Acta Agrobotanica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Prediction of protein subcellular localization using support vector machine with the choice of proper kernel
Autorzy:
Hasan, M.A.M.
Ahmad, S.
Molla, M.K.I.
Powiązania:
https://bibliotekanauki.pl/articles/81150.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
subcellular localization
protein
prediction
support vector machine
model selection
kernel
radial base function
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2017, 98, 2
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 31

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