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Tytuł:
Biochemical markers of bone turnover during pregnancy in horses: a longitudinal study
Autorzy:
Greiner, C.
Cavalier, E.
Remy, B.
Gabriel, A.
Farnir, F.
Gajewski, Z.
Carstanjen, B.
Powiązania:
https://bibliotekanauki.pl/articles/31744.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The effect of pregnancy on bone metabolism was investigated in healthy mares. Venous blood samples were collected 7 times from 19 multiparous mares starting at 20-weeks pre-parturition, continuing 6 times in 4-week intervals, including the week of parturition and one week after parturition. Serum concentrations of osteocalcin (OC) and carboxy-terminal cross-linking telopeptide of type I collagen (CTX-I) were determined. Measurement cycles and age had a significant (p < 0.01) influence on OC and CTX-I values. Pregnancy influenced bone metabolism with peak bone formation and resorption values around the time of parturition.
Źródło:
Polish Journal of Veterinary Sciences; 2012, 15, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Biochemical markers of oxidative stress in triticale seedlings exposed to cereal aphids
Autorzy:
Lukasik, I.
Golawska, S.
Powiązania:
https://bibliotekanauki.pl/articles/2117824.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
hydrogen peroxide
protein thiols
protein carbonyls
rhopalosiphum padi
sitobion
avenae
triticale
Opis:
In this study, we examined whether and to what extent oxidative stress is induced in seedlings of two winter triti- cale (Triticosecale wittm.) varieties (susceptible Tornado and resistant witon) in response to infestation by the cereal grain aphid (sitobion avenae l.) and bird-cherry-oat aphid (rhopalosiphum padi l.). we compared the level of hydrogen peroxide (H 2O2) and lipid peroxidation products as well as markers of protein damage (protein- bound thiol and carbonyl groups). The studied parameters were measured at 6, 24, 48 and 96 h post-initial aphid infestation compared to the non-infested control seedlings. Our studies indicated that the cereal aphid feeding evoked oxidative stress in the triticale seedlings. Cereal aphid feeding increased the H2O2 level in triticale tissues, with maximum levels observed at 24 and 48 h post-infestation. Triticale infestation with aphids also increased lipid peroxidation products in triticale seedlings, with the maximal levels at 48 or 96 h post-infestation. Further, there was a reduction in protein thiol content and an increase in protein carbonyl content in the triticale seedlings after infestation with female aphids. Stronger triticale macromolecule damages were evoked by the oligophagous aphid r. padi. There was a more substantial protein thiol content reduction in the resistant witon cultivar and higher accumulation of protein-bound carbonyls in the tissues of the susceptible Tornado cultivar. The changes were proportional to the aphid population and the time of aphid attack. These findings indicate that the defensive strategies against cereal aphid (s. avenae and r. padi) infestation were stimulated in triticale Tornado and witon seedlings. Our results explain some aspects and broaden the current knowledge of regulatory mechanisms in plant-aphid interactions.
Źródło:
Acta Biologica Cracoviensia. Series Botanica; 2019, 61, 2; 35-46
0001-5296
Pojawia się w:
Acta Biologica Cracoviensia. Series Botanica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The impact of physical effort on cortisol level: a review of the latest report
Autorzy:
Grzebisz, Natalia
Powiązania:
https://bibliotekanauki.pl/articles/1189840.pdf
Data publikacji:
2016
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
effort
cortisol
saliva
overtraining
biochemical markers
Opis:
The results of the current study demonstrate that exercise interferes with the hormonal balance system. The size and direction of these changes depend on the volume and intensity of a physical effort. Biochemical monitoring can support the process of training and gathered data can assist in countering the effects of overtraining. The article presents the latest reports on the character of the changes in cortisol influenced by various types of physical effort, the impact of the training units, time of their execution and the influence of the supply of carbohydrates on the level of this hormone in the body. This work also presents the advantages of saliva analysis, as a reliable material to measure the level of cortisol in athletes.
Źródło:
World Scientific News; 2016, 48; 205-210
2392-2192
Pojawia się w:
World Scientific News
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of host plants on biochemical markers of oxidative stress within tissues of Pea aphid
Autorzy:
Lukasik, I.
Golawska, S.
Wojcicka, A.
Powiązania:
https://bibliotekanauki.pl/articles/66913.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The effect of some species of the Fabaceae family (the pea Pisum sativum L., broad bean Vicia faba L. and vetch V. sativa L.) on biochemical markers of oxidative stress within tissues of the pea aphid, Acyrthosiphon pisum Harris (Homoptera, Aphididae), has been studied. The highest concentration of superoxide anion radical (O2-), hydrogen peroxide (H2O2) and lipid peroxidation products thiobarbituric acid reactive substances (TBARS) was recorded for morphs that fed on the broad bean. The opposite tendency was observed for the level of total thiols, that were the highest for insects reared on the pea. Among the studied aphid morphs, the highest concentration of superoxide anion and hydrogen peroxide was noted for the wingless females whereas TBARS content was the highest within the winged migrant tissues. Different results were obtained for the total thiols, where apterae as well as migrants reared on the tested plants, had comparable content of these compounds. Our experiments indicate that oxidative stress may play an important role in interactions between the pea aphid and their host plants.
Źródło:
Journal of Plant Protection Research; 2012, 52, 1
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Specialized Movement on the Rowing Ergometer and Post-workout Changes in Selected Peripheral Blood Parameters - a Case Report.
Autorzy:
Proia, Patrizia
Amato, Alessandra
Contró, Valentina
Thiel, Dan
Vostatková, Pavlína
Buryta, Beata
Kolbowicz, Marek
Lewandowski, Tomasz
Nowakowska, Anna
Powiązania:
https://bibliotekanauki.pl/articles/1030593.pdf
Data publikacji:
2018
Wydawca:
Uniwersytet Szczeciński. Wydawnictwo Naukowe Uniwersytetu Szczecińskiego
Tematy:
biochemical markers
haematological markers
matrix metalloproteinases
rowing
Opis:
Rowing is a sport discipline, which requires extreme physical strength and endurance and appropriate aerobic and anaerobic capacity as well. However, when the workout intensity and load is very high, exercise is associated with temporary changes in cellular metabolism and the immune system. The study included one male rower aged 28 years - the highly-skilled and experienced athlete. We determined basic cardiorespiratory fitness measures, complete blood count, and 24 clinical chemistry parameters including relevant biochemical and haematological parameters and matrix metaloproteinases activities. Maximal exercise on the rowing ergometer induced 2-fold increase in absolute counts of all leukocytes subsets. There was observed an increase in C-reactive protein concentrations as well. MMP-9 activity increased 1,3-fold compared to the baseline value. Exhaustive exercise caused significant changes in creatinine and urea serum levels, but the most prominent changes were found in total and direct bilirubin concentrations. Maximal exercise induced also a decrease in the iron and magnesium levels. No changes in ALT, GGT and ALP activity were observed, while increase in CK, AST and LDH activity in post-exercise time and the decrease during the recovery was found. Therefore acute specialized movement on the rowing ergometer is not the cause of muscular damage, but rather indicate efficient adaptation to the physical exercise. Moreover, it seems that maximal exercise induces an inflammatory response characterized by greater count of all subpopulations of leukocytes, elevated levels of CRP and MMP-9 serum activity.
Źródło:
Central European Journal of Sport Sciences and Medicine; 2018, 24, 4; 5-13
2300-9705
2353-2807
Pojawia się w:
Central European Journal of Sport Sciences and Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Semen characteristics and selected biochemical markers of canine seminal plasma in various seasons of the year
Autorzy:
Strzezek, R.
Szemplinska, K.
Filipowicz, K.
Kordan, W.
Powiązania:
https://bibliotekanauki.pl/articles/2087988.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The aim of this study was to evaluate the influence of season on selected qualitative semen characteristics and biochemical markers of canine seminal plasma. Whole ejaculates were collected from 5 crossbred dogs aged 2-8 years. The study covered a period of one year divided into four seasons: spring (March, April, May), summer (June, July, August), autumn (September, October, November) and winter (December, January, February). Semen samples were subjected to macroscopic and microscopic analyses to determine semen volume, total sperm counts and sperm morphology parameters. The study also involved the determination of sperm motility parameters (CASA system), sperm plasma membrane integrity (SPMI, fluorescent staining SYBR-14/PI), sperm mitochondrial membrane potential (MMP, fluorescent staining JC-1/PI) and the ATP content of sperm cells. Total protein content (TPC) and the activity of alkaline phosphatase (AP) and acid phosphatase (AcP) were determined in biochemical analyses of seminal plasma. No significant differences in ejaculate volume, SMPI or ATP content of sperm cells were observed between seasons. The highest total sperm counts were reported in ejaculates acquired in summer and autumn. The lowest MMP values were determined in summer ejaculates. No significant differences in sperm motility (MOT) were observed throughout the experiment, but ejaculates collected in autumn and winter were characterized by the highest progressive motility (PMOT). AP activity and TPC were not significantly affected by season. However, AcP activity levels were significantly lower in autumn than in the remaining seasons. Seasonal variations in the analyzed macroscopic and microscopic parameters of ejaculates and biochemical markers of seminal plasma did not exert a clear negative effect on the quality of canine semen.
Źródło:
Polish Journal of Veterinary Sciences; 2015, 18, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The dynamics of biochemical markers of enzymoemia and middle-weight molecules in the early post-traumatic period of brain injury
Autorzy:
Ziablitsev, S. V.
Elskyy, V. N.
Pischulina, S. V.
Strelchenko, U. I.
Antonov, E. V.
Sudilovskaya, U. L.
Khudoley, S. A.
Powiązania:
https://bibliotekanauki.pl/articles/1032378.pdf
Data publikacji:
2014
Wydawca:
Uniwersytet Kazimierza Wielkiego w Bydgoszczy
Tematy:
brain injury
biochemical markers
enzymoemia
middle-weight molecules
Opis:
In the experimental model of brain injury (BI) markers of enzymoemia (aminotransferases ALT and AST, alkaline and acid phosphatase (AlP and AcP), cathepsin D (CD)) and middle-weight molecules (MWM) were estimated. 3 hours after trauma there were increase of all enzymes that evidences about damage of cytoplasmic (ALT, AST, AlF) and lysosomal membranes (AcP, CD). During period of observation different dynamics of enzymatic changes occurred: plateau phase for ALT and CD and progressive increase of concentration with the maximum on the 5th day of the experiment for AlP and AcP. Increase of the level of MWM displayed the development of endogenous intoxication in the post-traumatic period. Relation between particular components of MWM was studied. It was shown that primary accumulation of nucleotide fraction and aromatic peptides is adverse factor of the course of post-traumatic period, development of endotoxicosis, and multiorgan failure.
Źródło:
Journal of Health Sciences; 2014, 4, 1; 297-302
1429-9623
Pojawia się w:
Journal of Health Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Rapid evaluation of germinability of primed China aster (Callistephus chinensis Ness.) seeds with physiological and biochemical markers
Autorzy:
Badek, B.
Romanowska-Duda, Z.
Grzesik, M.
van Duijn, B.
Powiązania:
https://bibliotekanauki.pl/articles/2057.pdf
Data publikacji:
2014
Wydawca:
Instytut Ogrodnictwa
Tematy:
enzyme activity
electrolyte leakage
seed germination
China aster
Callistephus chinensis
biochemical marker
physiological marker
Źródło:
Journal of Horticultural Research; 2014, 22, 2
2300-5009
Pojawia się w:
Journal of Horticultural Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Use biochemical markers to evaluate the quality of fresh and cryopreserved semen from the arctic fox (Vulpes lagopus)
Autorzy:
Stasiak, K.
Glogowski, J.
Demianowicz, W.
Kowalski, R.
Nowak-Tkaczyk, A.
Janicki, B.
Powiązania:
https://bibliotekanauki.pl/articles/30654.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The aim of this study was to use biochemical markers to evaluate the quality of fresh and cryopreserved semen from the arctic fox (Vulpes lagopus). Twenty-three manually collected ejaculates were analysed for the main indicators of semen quality (sperm concentration and ejaculate volume). Sperm motility and percentage of morphologically normal and abnormal spermatozoa were determined according to the stage of cryopreservation (fresh - measurement A; equilibrated - measurement B; frozen/thawed - measurement C). Furthermore, the seminal plasma and supernatants were analysed after equilibration and freeze/thawing for the activity of the enzymes alkaline phosphatase (ALP), acid phosphatase (AcP), lactate dehydrogenase (LDH) and aspartate aminotransferase (AspAT), and for the activity of acrosin inhibitors (AP). The mean concentration of sperm was 625.1 million/cm3, and ejaculate volume averaged 1.6 cm3. Seminal plasma was characterized by the highest activity of alkaline phosphatase (3.43×103 U/l) and lowest activity of acrosin inhibitors (4.55×103 U/l). After equilibration, the supernatants showed the highest activity of acid phosphatase (94.9 U/l) and after freeze-thawing, they showed a high activity of lactate dehydrogenase (535.8 U/l) and aspartate aminotransferase (577.1 U/l), which indicates that these proteins had leaked from spermatozoa into the extracellular medium during the biotechnique of semen cryopreservation. In addition, several significant relationships were found between some indicators of semen quality and plasma and/or supernatant enzyme activity.
Źródło:
Polish Journal of Veterinary Sciences; 2014, 17, 3
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Identification of glutenin and secalin subunits in addition and substitution lines of wheat (Triticum aestivum L.) using biochemical markers
Autorzy:
Chrząstek, Maria
Powiązania:
https://bibliotekanauki.pl/articles/2199010.pdf
Data publikacji:
2005-12-22
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
addition lines
HMW glutenins
LMW glutenins
1B/1R substitution line
SDS-PAGE electrophoresis
secalins
Opis:
The aim of paper was evaluation of composition of high molecular weight (HMW) and low molecular weight (LMW) glutenin subunits in wheat-rye addition lines cv. Grana - cv. Dańkowskie Złote, substitution line 1B/1R and initial forms, as well as, localization of Sec genes on chromosomes ‘Dańkowskie Złote’ rye. Analysis were performed using SDS-PAGE electrophoresis.The composition of HMW glutenin for all addition lines and ‘Grana’ wheat was: N - 6+8 - 2+12. Composition of LMW glutenin was estimated only for 2R and 6RL addition lines, wheat, and octoploid triticale. In both addition lines the subunits N-6+9-15 were identified, in wheat cv. Grana subunits 1-6+9-15 were present, whereas in octoploid triticale polymorphism was observed (1-6+9-15 or 1-11+13-N). Rye storage proteins (secalins) was found in addition lines: 1R, 2R and substitution line 1B/1R. It can be concluded that genes responsible for secalin biosynthesis in rye cv. Dańkowskie Złote are localized on 1R and 2R chromosomes.
Źródło:
Plant Breeding and Seed Science; 2005, 52; 79-86
1429-3862
2083-599X
Pojawia się w:
Plant Breeding and Seed Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Możliwości praktycznego zastosowania nowych wskaźników biochemicznych nadużywania alkoholu etylowego
New biochemical markers of ethyl alcohol abuse – new possibilities in clinical practice
Autorzy:
Paul, Przemysław
Kanclerz, Katarzyna
Kubanek, Alicja
Renke, Joanna
Renke, Marcin
Powiązania:
https://bibliotekanauki.pl/articles/2082697.pdf
Data publikacji:
2021-04-09
Wydawca:
Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
Tematy:
alkohol
wskaźniki
medycyna pracy
ubogowęglowodanowe izoformy transferyny
glukuronian etylu
fosfatydyloetanol
alcohol
biomarkers
occupational medicine
carbohydrate-deficient transferrin isoforms
ethyl glucuronide
phosphatidylethanol
Opis:
Alkohol jest w krajach rozwiniętych jednym z głównych czynników behawioralnych utraty zdrowia i życia. Jego nadużywanie ma poważne skutki społeczne i ekonomiczne: przyczynia się do większej liczby wypadków w pracy, kolizji drogowych oraz nieobecności w zakładach pracy. Rozpoznawanie i leczenie alkoholizmu jest bardzo trudne, dlatego wykorzystanie obiektywnych wskaźników biochemicznych nadużywania alkoholu etylowego może przyczynić się do wcześniejszego rozpoznawania, skuteczniejszego leczenia i wiarygodnego monitorowania okresu abstynencji. Celem pracy jest przedstawienie dostępnych w Polsce czułych i swoistych wskaźników szkodliwego spożywania alkoholu ze szczególnym uwzględnieniem możliwości ich praktycznego wykorzystania. Takie testy mogą znaleźć zastosowanie m.in. w programach odzyskiwania prawa jazdy przez kierowców zatrzymanych z powodu jazdy pod wpływem alkoholu, wczesnego wykrywania osób nadużywających alkoholu wśród pracowników podczas rutynowych badań w zakładach pracy, do monitorowania abstynencji w trakcie leczenia odwykowego, przed planowanymi zabiegami przeszczepiania narządów, do wykrywania szkodliwego spożywania alkoholu wśród kobiet ciężarnych i w sekcjach sądowo-lekarskich. Niezbędna jest standaryzacja metod oznaczania wskaźników w materiale biologicznym, a istotnym problemem we właściwej interpretacji wyników mogą być choroby współistniejące. Mimo tych ograniczeń obiektywne wskaźniki biochemiczne nadużywania alkoholu etylowego mogą być pomocne w opiece nad pacjentami. Szczególną rolę mogą pełnić w diagnostyce w medycynie pracy, przyczyniając się do wzrostu bezpieczeństwa na drogach publicznych oraz bezpieczeństwa pracowników w zakładach pracy.
Alcohol ranks as one of the leading behavioral threats to health and life in developed countries. Alcohol abuse triggers serious social and economic effects: it contributes to higher prevalence of work-related and road accidents, as well as absence from work. The diagnosis and treatment of alcoholism still remain very difficult. Hence, the use of objective biochemical markers of alcohol abuse may contribute to earlier detection, more effective therapy and reliable teetotalism control. The aim of this study is to present the sensitive and specific biomarkers of alcohol abuse available in Poland, with particular emphasis on the practical use possibilities. Such tests may be widely used, e.g., in driving license regranting cases involving drivers whose licenses were suspended for driving when intoxicated, for the early detection of persons abusing alcohol in employment-related health controls, for abstinence monitoring during withdrawal treatment, for detecting alcohol consumption in transplant settings, for assessing the prevalence of alcohol drinking in pregnancy, as well as in autopsical examinations. The standardization of biomarkers measurement methods is essential. Moreover, concomitant disorders may pose a significant problem in the proper outcome analysis. Despite these limitations, objective biochemical markers of ethyl alcohol abuse may become helpful tools in medical care. They can play a particular role in occupational medicine diagnostics, contributing to the higher level of safety on public roads and to worker safety.
Źródło:
Medycyna Pracy; 2021, 72, 2; 173-184
0465-5893
2353-1339
Pojawia się w:
Medycyna Pracy
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Occupational daily walking steps have inverse relationship with papillary thyroid cancer risk and progression: a retrospective analysis
Autorzy:
Zhang, Chunling
Zhang, Li
Su, Yan
Xiang, Lei
Zhang, Qin
Zhang, Chunhong
Huan, Qin
Powiązania:
https://bibliotekanauki.pl/articles/28761990.pdf
Data publikacji:
2024-03-05
Wydawca:
Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
Tematy:
biochemical markers
office workers
construction workers
papillary thyroid cancer
daily step counts
PTC stages
Opis:
Objectives Investigate the impact of daily occupational walking steps on the progression of papillary thyroid cancer (PTC), a topic hitherto underresearched. Material and Methods The authors analyzed the data from 800 individuals with PTC across stages 0–IV. Participants were evenly divided into 2 distinct occupational groups: office workers and construction workers (N = 400 each). Data included comprehensive records of daily walking steps, demographic information, and clinical indicators. Pearson’s correlation coefficients or analysis of variance (ANOVA) were employed to assess the linkage between daily walking steps and PTC risk and stage, as well as associated biochemical markers. Results The analysis revealed a significant inverse relationship between daily walking steps and PTC risk. A higher frequency of daily steps was associated with reduced chances of PTC onset and a lower diagnostic stage of the disease. This protective effect of physical activity was particularly pronounced in the construction worker cohort. Subsequent evaluations showed that construction workers who consistently logged higher daily steps had markedly lower levels of thyroid-stimulating hormone (TSH), free triiodothyronine, free thyroxine, thyroid peroxidase antibody, thyroglobulin antibody, and thyroglobulin (Tg). Notably, daily walking steps exhibited a strong inverse correlation with body mass index (BMI), age, PTC volumes, and levels of TSH and Tg across both occupational groups (ρ < –0.37). The increase in daily steps was associated with the reduction in PTC stages (p < 0.001). Conclusions The research underscores the potential benefits of increased daily walking steps, suggesting that they may play a protective role in reducing PTC risk and moderating its progression.
Źródło:
International Journal of Occupational Medicine and Environmental Health; 2024, 37, 1; 58-71
1232-1087
1896-494X
Pojawia się w:
International Journal of Occupational Medicine and Environmental Health
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effects of Long-Term Dietary Administration of Kale (Brassica oleracea L. var. acephala DC) Leaves on the Antioxidant Status and Blood Biochemical Markers in Rats
Autorzy:
Papierska, Katarzyna
Ignatowicz, Ewa
Jodynis-Liebert, Jadwiga
Kujawska, Małgorzata
Biegańska-Marecik, Róża
Powiązania:
https://bibliotekanauki.pl/articles/2159287.pdf
Data publikacji:
2022-09-05
Wydawca:
Instytut Rozrodu Zwierząt i Badań Żywności Polskiej Akademii Nauk w Olsztynie
Tematy:
antioxidant status
dietary antioxidants
functional food
kale
Opis:
Kale (Brassica oleracea L. var. acephala DC) is a leafy green vegetable which belongs to the Brassicaceae family, one of the most commonly cultivated and consumed edible plants. The aim of this study was to investigate the potential adverse effects and antioxidant properties of freeze-dried kale leaves in 90-day dietary experiment on Wistar rats. The kale leaf powder was added to a diet at levels: 10, 30, and 60 g/kg feed. The standard blood biochemical and hematological markers, antioxidant enzyme activities, level of lipid peroxidation, reduced glutathione content, and damage to DNA in the liver were measured. Antioxidant potential in serum was measured and expressed as Trolox equivalents antioxidant capacity (TEAC). The kale leaf phytochemicals, i.e., glucosinolates, phenolic acids and flavonols, were quantified. Major glucosinolates were glucoiberin (164.6 mg/100 g d.w.) and glucobrassicin (130.6 mg/100 g d.w.), whereas kaempferol (159.1 mg/100 g d.w.), quercetin (119.6 mg/100 g d.w.), and sinapic acid (73.8 mg/100 g d.w.) dominated among phenolics. The lowest dose of kale leaves augmented the activity of catalase by 34% in males and by 44% in females. The highest applied dose increased activities of glutathione reductase by 31%, superoxide dismutase by 27%, and glutathione S-transferase by 24% in males only. Kale administration did not affect the activities of glutathione peroxidase and paraoxonase 1, glutathione concentration, lipid peroxidation level, and the level of DNA damage in the liver and the whole blood leukocytes. TEAC increased upon all doses of kale leaves by 30–90% in males and by 40–90% in females. The diet with kale did not affect blood biochemical and hematological markers. Results confirm no adverse effects of dried kale leaves used in model rat’s diet up to 60 g/kg for 90 days. The changes in hepatic antioxidant enzymes and plasma TEAC suggest beneficial effect of kale leaves on the antioxidant status in rats. Interestingly, these changes were more pronounced in male versus female animals.
Źródło:
Polish Journal of Food and Nutrition Sciences; 2022, 72, 3; 239-247
1230-0322
2083-6007
Pojawia się w:
Polish Journal of Food and Nutrition Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Response of vitamins A, E, hematological and serum biochemical markers in Crucian carp (Carassius auratus gibelio) exposed to environmental Pb2+ and Cd2+
Autorzy:
Khan, Sher
Zhou, Peiyuan
Liu, Xiaoyu
Li, Hong
Li, Jingna
Rehman, Zia ur
Ahmad, Ijaz
Powiązania:
https://bibliotekanauki.pl/articles/1039010.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
vitamins A and E
hematology
serum chemistry
Crucian carp (Carassius auratus gibelio)
Cd2+
Pb2+
Opis:
Toxicity of Pb2+ and Cd2+ is a widespread issue in the world; however, few studies have been conducted to understand their effect at environmentally realistic concentration in a mixture. In the present study, Crucian carp was exposed to Pb2+ (30 µg·l-1), Cd2+ (100 µg·l-1) and their mixture (30+100 µg·l-1) for 96 h and 21 d period to assess changes in the liver and muscle vitamin A and E content, and hematological and serum biochemical parameters. The results indicated significant decline in the level of antioxidant vitamins A, E and alterations in the hematological and serum biochemical indices. The toxicity revealed anemia, impairment of the liver and kidney with evident responses after 21 d exposure due to additive effect of Pb2+ and Cd2+ in mixture. Moreover, the differential response of vitamins A, E and blood parameters to low levels of waterborne Pb2+ and Cd2+ in freshwater fish can be used as biomarkers for monitoring contamination of aquatic environment.
Źródło:
Acta Biochimica Polonica; 2015, 62, 3; 581-587
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Impact of products from ground buckwheat added to balanced diets on biochemical blood markers in Wistar rats
Autorzy:
Orzel, D.
Zmijewski, M.
Bronkowska, M.
Powiązania:
https://bibliotekanauki.pl/articles/876652.pdf
Data publikacji:
2015
Wydawca:
Narodowy Instytut Zdrowia Publicznego. Państwowy Zakład Higieny
Tematy:
buckwheat
product
addition
diet
human nutrition
biochemical marker
blood marker
Wistar rat
rat
cholesterol
triacylglycerol
functional food
human health
Opis:
Background. It is believed that buckwheat can be used as a functional food ingredient in the prevention and treatment of diet-related diseases, e.g., atherosclerosis, hypertension, obesity, constipation and cancers. The use of buckwheat protein preparations in the diet for experimental animals had a significant effect on the reduction of cholesterol level in their blood serum, liver and gall bladder, and additionally inhibited the formation of gall stones as a result of changes in cholesterol metabolism. Buckwheat protein extracts have additionally been shown to reduce the level of LDL and VLDL fractions. Objective. The aim of this study was to evaluate the effects of different products from ground buckwheat nuts (flour, meal and bran) and bread with their addition applied in balanced diets on selected biochemical blood markers in Wistar rats. Material and Methods. The study was conducted with 64 male Wistar rats. Animals received a semi-synthetic diet AIN 93M with 20% addition of buckwheat flour, meal or bran and containing 20% rye-buckwheat bread made with 35% buckwheat flour, meal or bran, respectively. The animals were fed the experimental diets for 4 weeks. Whole blood was used to measure hematocrit and hemoglobin concentrations. The contents of glucose, total cholesterol, LDL, HDL, and triglycerides were determined in the blood serum using BioSystem biochemical tests. Results. There was no significant effect of 20% addition of ground buckwheat products (buckwheat flour, meal and bran) or bread with their addition to balanced diets on hematocrit, hemoglobin concentration in the blood or the concentration of glucose in the blood serum of experimental animals. The study showed a statistically significant beneficial effect of dietary addition of buckwheat or rye-buckwheat bread on the reduction of total cholesterol, LDL cholesterol, and triglycerides (TGC) in the blood serum of rats. Conclusions. A statistically significant decrease was demonstrated in LDL cholesterol and triglycerides in the blood serum of animals fed diets with 20% addition of ground buckwheat products (buckwheat flour, meal and bran) and rye-buckwheat bread containing these products.
Wprowadzenie. Uważa się, że gryka może znaleźć zastosowanie, jako składnik żywności funkcjonalnej w prewencji i leczeniu chorób dietozależnych, m.in. miażdżycy, nadciśnienia, otyłości, zaparć, a także chorób nowotworowych. Zastosowanie preparatów z gryki w diecie zwierząt doświadczalnych w znaczący sposób wpływa na obniżenie stężenia cholesterolu w surowicy krwi, wątrobie oraz woreczku żółciowym, a ponadto hamuje powstawanie kamieni żółciowych na skutek zmiany metabolizmu cholesterolu. Wykazano, że produkty z gryki obniżają poziom frakcji cholesterolu LDL i VLDL. Cel. Celem niniejszych badań była ocena wpływu rożnych produktów przemiału orzeszków gryki (mąki, śruty i otrąb) oraz pieczywa z ich udziałem, dodawanych do diet zbilansowanych, na wybrane wskaźniki biochemiczne krwi (hematokryt, stężenie hemoglobiny we krwi oraz stężenie glukozy, cholesterolu całkowitego, cholesterolu HDL i LDL oraz triglicerydów w surowicy krwi) u szczurów rasy Wistar. Materiał i metody. Badania prowadzono z udziałem 64 samców szczurów rasy Wistar. Zwierzęta otrzymywały diety AIN 93M zmodyfikowane z 20% dodatkiem odpowiednio - mąki, śruty lub otrąb gryczanych oraz z 20% dodatkiem pieczywa żytnio-gryczanego odpowiednio z 35% udziałem mąki, śruty lub otrąb gryczanych. Zwierzęta karmiono dietami doświadczalnymi przez 4 tygodnie. W krwi pełnej oznaczano: wskaźnik hematokrytu i stężenie hemoglobiny. Zawartość glukozy, cholesterolu całkowitego, cholesterolu LDL, HDL i triglicerydów oznaczano w surowicy krwi. Wyniki. Nie wykazano istotnego wpływu 20% dodatku produktów przemiału orzeszków gryki (mąki, śruty i otrąb gryczanych) oraz pieczywa z ich udziałem do diet zbilansowanych na wartości hematokrytu, stężenie hemoglobiny we krwi oraz stężenie glukozy w surowicy krwi badanych zwierząt doświadczalnych. Wykazano statystycznie istotny korzystny wpływ dodatku gryki lub pieczywa żytnio – gryczanego na obniżenie zawartości cholesterolu całkowitego, cholesterolu LDL oraz triglicerydów (TGC) w surowicy krwi. Wnioski. Stwierdzono statystycznie istotne obniżenie stężenia cholesterolu LDL oraz triglicerydów w surowicy krwi zwierząt, karmionych dietami z 20% dodatkiem produktów przemiału orzeszków gryki (mąki, śruty i otrąb gryczanych) oraz pieczywa żytnio - gryczanego z ich udziałem.
Źródło:
Roczniki Państwowego Zakładu Higieny; 2015, 66, 3
0035-7715
Pojawia się w:
Roczniki Państwowego Zakładu Higieny
Dostawca treści:
Biblioteka Nauki
Artykuł
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