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Tytuł:
Isolation and genetic analysis of a variant porcine epidemic diarrhea virus in China
Autorzy:
Li, R.-f.
Tian, X.-g.
Liu, Y.
Xu, J.
Liu, D.-y.
Powiązania:
https://bibliotekanauki.pl/articles/30492.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Porcine epidemic diarrhea virus (PEDV) is having a severe effect on the pig breeding industry in central China. The mucosa and the content of the small intestine from newborn pre-weaned piglets with diarrhea were tested for the presence of PEDV by molecular and morphologic methods, and found to be positive. Negative-staining electron microscopy (EM) revealed the presence of coronavirus- like particles in the samples. The result of molecular detection by nested RT-PCR based on the amplification of the M gene was positive. Using a novel alternative method we successfully propagated the PEDV strain (CH/QX-2) in Vero cells, confirmed by ultrathin sections of the cells and Immunofluorescence assay (IFA). Phylogenetic analysis based on the partial S gene showed that the CH/QX-2 isolate was genetically closer to strains more commonly found in China, but differed genetically from two domestic strains (CH/S, 1986 and LZC, 2007), Korean strains (DR13, 2007), and the vaccine strain (CV777 vs) currently being used in China. CH/QX-2 formed a unique clade in the derived phylogenetic tree indicating that the CH/QX-2 strain currently circulating in central China is a new variant of PEDV. This study extends current knowledge on the diversity and epidemiology of PEDV.
Źródło:
Polish Journal of Veterinary Sciences; 2016, 19, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Generation and characterization of a monoclonal antibody against duck Tembusu virus envelope protein
Autorzy:
Han, K.
Zhao, D.
Liu, Y.
Liu, Q.
Huang, X.
Yang, J.
Bi, K.
Xu, T.
Li, Y.
Powiązania:
https://bibliotekanauki.pl/articles/31495.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Duck Tembusu virus (DTMUV) is a newly emerging pathogenic flavivirus that has caused massive economic losses to the duck industry in China. Envelope (E) protein of DTMUV is an important structural protein, which is able to induce protective immune response in target animals and can be used as specific serological diagnosis tool. In this study, a novel monoclonal antibody, designated mAb 3E9, was generated against DTMUV E protein. It is positive in indirect ELISA against both His-E protein and the purified whole viral antigen. Also, this mAb showed positive reaction with DTMUV in Western blot and indirect immunofluorescence assay, and the isotype was IgG1. End-point neutralizing assay performed in BHK-21 cells revealed that the neutralization titer of 3E9 against DTMUV JS804 strain reached 1:50. Furthermore, functional studies revealed that 3E9 blocks infection of DTMUV at a step on viral attachment. The anti-E mAbs produced in the present work may be valuable in developing an antigen-capture ELISA test for antigen detection or a competitive ELISA test for antibody detection or therapeutic medicine for DTMUV in poultry.
Źródło:
Polish Journal of Veterinary Sciences; 2016, 19, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Research on a New Synthesis of LLM-105 Using N-Nitroso-bis(cyanomethyl)amine
Autorzy:
Jing, S.
Liu, Y.
Liu, D.
Guo, J.
Powiązania:
https://bibliotekanauki.pl/articles/358371.pdf
Data publikacji:
2016
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
Tematy:
LLM-105
energetic materials
synthesis
characterization
properties
Opis:
A new three-step synthetic method was designed to prepare 2,6-diamino- 3,5-dinitropyrazine 1-oxide (LLM-105) with an overall yield of 43.5%, using iminodiacetonitrile, triethylamine and hydroxylamine hydrochloride as the starting materials, and involved nitrosylation, cyclization and nitration. The structure of LLM-105 was characterized by FTIR, 1H NMR spectroscopy and elemental analysis. The crucial factors and mechanism of the cyclization were explored. Thermal decomposition, mechanical sensitivity and the purity of the LLM-105 were determined by TG-DTA, an impact sensitivity instrument and high performance liquid chromatography (HPLC) respectively. The results showed that LLM-105 synthesised via 2,6-diamino-3,5-dinitropyrazine (ANPZ) was less sensitive to impact, but the LLM-105 synthesized by the new method had some obvious advantages in terms of purity, security and production costs. Moreover, the LLM-105 synthesized by this new method can be initiated reliably without recrystallization.
Źródło:
Central European Journal of Energetic Materials; 2016, 13, 1; 21-32
1733-7178
Pojawia się w:
Central European Journal of Energetic Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of experimental infections of various Tembusu virus strains isolated from geese, ducks and chickens on ducklings
Autorzy:
Xu, T.
Huang, X.
Li, Y.
Liu, Q.
Liu, X.
Han, K.
Liu, Y.
Yang, J.
Zhao, D.
Bi, K.
Sun, W.
Powiązania:
https://bibliotekanauki.pl/articles/2087719.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
goose-derived
duck-derived
chicken-derived
Tembusu virus
pathogenicity
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 2; 389-396
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Looped-chain-based active current sharing strategy in DC microgrids
Autorzy:
Li, D.
Liu, Y.
Gu, Y.
Qi, H.
Powiązania:
https://bibliotekanauki.pl/articles/141109.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
active current sharing
DC microgrids
looped control diagram
Opis:
The integration of renewable energy sources in modern electric grids have drawn increasing attention nowadays. In order to effectively manage large-scale renewable energy sources and achieve flexible and efficient operation, the concept of microgrids have been proposed. Considering the nature of DC outputs in many distributed energy resources (DERs), DC microgrids have been extensively studied in the past years. Among the operational issues in DC microgrids, current sharing issues have become an important topic since it is highly relevant to the operation of DC microgrids. By adopting a proper design of current sharing strategy in DC microgrids, the current rating violations in each interface converter can be successfully avoided. In this paper, a looped-chainbased active current sharing strategy is proposed to realize high accuracy current sharing in DC microgrids. In particular, the output current is shared between the neighboring interface converters. Hence, following a clockwise or counter-clockwise order, a loopedchain- based control diagram can be established to share the reference value of the output current. A final status in the whole DC microgrid is that the output current of every interface converter is equalized. Hence, the desired current sharing objective can be satisfied. A MATLAB simulation model is established to verify the proposed loopedchain- based active current sharing strategy in DC microgrids.
Źródło:
Archives of Electrical Engineering; 2017, 66, 3; 559-569
1427-4221
2300-2506
Pojawia się w:
Archives of Electrical Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Identification of a linear epitope in the capsid protein of goose astrovirus with monoclonal antibody
Autorzy:
Dai, G.
Huang, X.
Liu, Q.
Li, Y.
Zhang, L.
Han, K.
Yang, J.
Liu, Y.
Xue, F.
Zhao, D.
Powiązania:
https://bibliotekanauki.pl/articles/16647377.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
epitope
goose astrovirus
capsid protein
monoclonal antibody
Opis:
Goose astrovirus (GoAstV) is a novel avastrovirus that typically causes gosling gout and results in 2 to 20% mortality. GoAstV capsid protein is the sole structural protein, which is responsible for viral attachment, assembly, maturation as well as eliciting host antibodies. However, the epitopes within capsid protein have not been well studied. In this study, a monoclonal antibody, named 1D7, was generated against GoAstV capsid protein by hybridoma technology. Western blot results showed that this MAb could react with recombinant capsid protein expressed in E. coli. Also, it recognized the precursor of capsid protein, VP90 and VP70, in GoAstV-infected cells. Besides, excellent specificity of MAb 1D7 was further demonstrated in indirect immunofluorescence assay and immunohistochemical analysis. Epitope mapping results revealed that MAb 1D7 recognized the epitope 33QKVY 36 within Cap protein. Sequence alignment indicated that 33QKVY 36 is a conserved epitope among the isolates of goose astrovirus type 2 (GoAstV-2), suggesting the potential for its use in GoAstV-2 specific diagnostic assay. These findings may provide some insight into a function of the GoAstV capsid protein and further contribute to the development of diagnostic methods for GoAstV infection.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 4; 579-587
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
New data on the anatomy of fuxianhuiid arthropod Guangweicaris spinatus from the lower Cambrian Guanshan Biota, Yunnan, China
Autorzy:
Chen, H.
Legg, D.A.
Zhai, D.-Y.
Liu, Y.
Hou, X.-G.
Powiązania:
https://bibliotekanauki.pl/articles/2082130.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Instytut Paleobiologii PAN
Tematy:
Arthropoda
Euarthropoda
Fuxianhuiida
Fuxianhuiidae
Guanshan Biota
Cambrian
Asia
Opis:
The fuxianhuiid arthropod Guangweicaris spinatus, from the lower Cambrian (Series 2, Stage 4), Guanshan Biota (Wulongqing Member, Canglangpu Formation), located in the vicinity of Kunming (Yunnan Province, southwest China), is redescribed based on new specimens and a re-examination of previously described material. A more complete overview of its morphology is given. Newly recognised features include: (i) a medial cephalic bulge; (ii) a tripartite hypostome; (iii) a pair of specialized post-antennal appendage (SPA); (iv) a putative telson; (v) two pairs of spines on the posteroventral margin of the terminal abdominal segment. This information is used to provide an emended diagnosis of the family Fuxianhuiidae, and the genus Guangweicaris.
Źródło:
Acta Palaeontologica Polonica; 2020, 65, 1; 139-148
0567-7920
Pojawia się w:
Acta Palaeontologica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Biological characteristics of a new antibacterial peptide and its antibacterial mechanisms against Gram-negative bacteria
Autorzy:
Pei, Z.
Ying, X.
Tang, Y.
Liu, L.
Zhang, H.
Liu, S.
Zhang, D.
Wang, K.
Kong, L.
Gao, Y.
Ma, H.
Powiązania:
https://bibliotekanauki.pl/articles/2087656.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
antimicrobial peptide
biological characteristics
antibacterial mechanism
Gram-negative bacteria
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 3; 533-542
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Experimental Study of the Explosion of Aluminized Explosives in Air
Autorzy:
Chen, Y.
Xu, S.
Wu, D. J.
Liu, D. B.
Powiązania:
https://bibliotekanauki.pl/articles/358676.pdf
Data publikacji:
2016
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
Tematy:
explosion in air
aluminized explosives
boron powders
TNT equivalence
fireballs
Opis:
Piezoelectric gauges were used to measure the shock wave overpressure of aluminized explosives and of a TNT charge. An infrared thermal-imaging spectrometer was used to collect the infrared signatures produced by the explosion fireball when the examined explosives were detonated. The measurement of the infrared signatures was used to estimate the surface temperatures and the dimensions of the fireball. Two aluminized explosive compositions (RDX/Al/AP and RDX/Al/B/AP) have been analyzed. 500 g charges of the aluminized explosives were prepared and studied, and their TNT equivalences were calculated according to the experimental data and the explosion law. The highest surface temperatures of the fireballs of these aluminized explosives were up to 1600 °C, which was higher than that of the TNT charge. In the region of the highest surface temperature above 700 °C, the duration for the composition RDX/Al/AP was about 232 ms (2.73 times more than TNT), whilst RDX/Al/B/AP was about 360 ms. The fireballs obtained from the explosion of these aluminized explosives had larger dimensions than that of TNT, especially when the surface temperature was above 1000 °C. The test results indicate that the addition of boron powders to aluminized explosives is a good way to enhance their blast effect, to improve the temperature of the explosion field and to prolong the duration of the higher temperature.
Źródło:
Central European Journal of Energetic Materials; 2016, 13, 1; 117-134
1733-7178
Pojawia się w:
Central European Journal of Energetic Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Development of a SYBR Green I real-time PCR assay for detection of novel porcine parvovirus 7
Autorzy:
Li, Y.D.
Yu, Z.D.
Bai, C.X.
Zhang, D.
Sun, P.
Peng, M.L
Liu, H.
Wang, J.
Wang, Y.
Powiązania:
https://bibliotekanauki.pl/articles/2087207.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Capsid gene
PPV7
SYBR Green I real-time PCR
Źródło:
Polish Journal of Veterinary Sciences; 2021, 24, 1; 43-49
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
3-D simulation of vertical-axial tidal current turbine
Autorzy:
Zhang, Z.
Ma, Y.
Jiang, J.
Liu, W.
Ma, Q.
Powiązania:
https://bibliotekanauki.pl/articles/260421.pdf
Data publikacji:
2016
Wydawca:
Politechnika Gdańska. Wydział Inżynierii Mechanicznej i Okrętownictwa
Tematy:
tidal current energy
vertical-axial turbine
hydrodynamic performance
CFD
numerical simulation
3-D effect
Opis:
Vertical-axial tidal current turbine is the key for the energy converter, which has the advantages of simple structure, adaptability to flow and uncomplex convection device. It has become the hot point for research and application recently. At present, the study on the hydrodynamic performance of vertical-axial tidal current turbine is almost on 2-D numerical simulation, without the consideration of 3-D effect. CFD (Computational Fluid Dynamics) method and blade optimal control technique are used to improve accuracy in the prediction of tidal current turbine hydrodynamic performance. Numerical simulation of vertical-axial tidal current turbine is validated. Fixed and variable deflection angle turbine are comparatively studied to analysis the influence of 3-D effect and the character of fluid field and pressure field. The method, put the plate on the end of blade, of reduce the energy loss caused by 3-D effect is proposed. The 3-D CFD numerical model of vertical-axial tidal current turbine hydrodynamic performance in this study may provide theoretical, methodical and technical reference for the optimal design of turbine.
Źródło:
Polish Maritime Research; 2016, 4; 73-83
1233-2585
Pojawia się w:
Polish Maritime Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Infuence of Iron Ion on Thermal Behavior of Ammonium Nitrate and Emulsion Explosives
Autorzy:
Xu, Z.
Liu, D.
Hu, Y.
Ye, Z.
Wei, Y.
Powiązania:
https://bibliotekanauki.pl/articles/357932.pdf
Data publikacji:
2010
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
Tematy:
Accelerating Rate Calorimeter (ARC)
ammonium nitrate
critical temperature
emulsion explosives
thermal stability
Opis:
The infuence of ferric nitrate on the thermal stability of ammonium nitrate and emulsion matrix were used to study with Accelerating Rate Calorimeter (ARC) and constant temperature thermal decomposition experiment in this paper. It is shown that ferric nitrate has signifcant catalytic property to the ammonium nitrate and emulsion matrix, which decrease the initial decomposition temperature of ammonium nitrate by 60.16 C and emulsion matrix by 30.33 C. The results of the infuence of ferric nitrate on catalysis of ammonium nitrate using ARC and constant temperature thermal decomposition experiment are the same. The most probable mechanism function, activation energy and the preexponential factor of ammonium nitrate with ferric nitrate were determined to be f(α) = 1-α, E = 195.41 kJ/mol, A = 2.08×1019 s-1. The kinetics of ammonium nitrate decomposition can be used to calculate the critical temperatures for ammonium nitrate decomposition with and without the presence of ferric nitrate, based on the Frank-Kamenetskii model of thermal explosion. Furthermore, as the iron-plate experiment shows, ferric nitrate can signifcantly reduce the critical temperature of emulsion matrix decomposition. Once the ferric nitrate interfuses in the ammonium nitrate and emulsion matrix, the thermal stability of ammonium nitrate and emulsion matrix is decreasing.
Źródło:
Central European Journal of Energetic Materials; 2010, 7, 1; 77-93
1733-7178
Pojawia się w:
Central European Journal of Energetic Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Study on 3-D Flow Patterns in Yarn Channels of an Interlacer
Badania charakterystyki przepływu 3D w dyszy komory przędącej urządzenia przeplatającego
Autorzy:
Qiu, H
Liu, Y.
Zhang, Y.
Powiązania:
https://bibliotekanauki.pl/articles/233558.pdf
Data publikacji:
2014
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Biopolimerów i Włókien Chemicznych
Tematy:
interlacer
flow patterns
computational fluid dynamics (CFD)
performance
przepływ strumienia powietrza
nawiew
obliczeniowa dynamika płynów
wydajność
Opis:
The aim of the present work was to determine the flow characteristics of compressible airflow in the yarn channel of an interlacer by numerical simulations to make clear the effects of cross-sectional shapes of the yarn channel on the performance of interlacers. CFD (Computational Fluid Dynamics) software package ANSYS CFX was used to calculate flow patterns in the yarn channel. Relations between the performance of the interlacer and distribution of the velocity vector, airflow speed and particle trace of the flow were clarified to provide knowledge to design better interlacers.
Celem pracy było określenie charakterystyk przepływu strumienia powietrza w dyszy komory przędącej urządzeń przeplatających za pomocą numerycznych symulacji dla wyjaśnienia wpływu kształtu przekroju kanałów prowadzących przędze na jakość pracy urządzenia przeplatającego. Program CFD (komputerowe określanie dynamiki przepływu) oparty na ANSYS CFX stosowano dla obliczeń przebiegów przepływu w dyszach przędzy. Dla uzyskania wiedzy potrzebnej dla lepszego projektowania urządzeń przeplatających badano zależności pomiędzy pracą tych urządzeń, rozkładem wektorów prędkości, szybkości przepływu oraz kształtu strumieni.
Źródło:
Fibres & Textiles in Eastern Europe; 2014, 1 (103); 56-61
1230-3666
2300-7354
Pojawia się w:
Fibres & Textiles in Eastern Europe
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Synthesis and Theoretical Studies of a New High Explosive, N,N,-Bis(3-aminofurazan-4-yl)-4,4´-diamino- 2,2´,3,3´,5,5´,6,6´-octanitroazobenzene
Autorzy:
Jing, S.
Liu, Y.
Liu, D.
Guo, J.
Powiązania:
https://bibliotekanauki.pl/articles/358642.pdf
Data publikacji:
2015
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
Tematy:
BAFDAONAB
density functional theory
detonation properties
Opis:
A new high energy, low sensitivity material N,N,-bis(3-aminofurazan- 4-yl)-4,4´-diamino-2,2´,3,3´,5,5´,6,6´-octanitroazobenzene (BAFDAONAB) was synthesized from 4-chlorobenzoic acid and diaminofurazan. The structure of BAFDAONAB was characterized by FT-IR, NMR and Elemental Analyses and its purity was determined by HPLC. The optimized structure and thermodynamic parameters were obtained at the DFT- B3LYP/6-31+G** theoretical level. Its detonation properties were also predicted by the formulae of Kamlet-Jacobs and the Monte-Carlo method. The results show that the density, heat of formation, detonation velocity, detonation pressure, impact sensitivity and purity were 1.93 g/cm3, 4487.44 kJ/mol, 9.01 km/s, 35.03 GPa, 63 cm and 99.3%, respectively. In addition, the compound was an insensitive high explosive which could meet the requirements of high energetic materials.
Źródło:
Central European Journal of Energetic Materials; 2015, 12, 4; 745-755
1733-7178
Pojawia się w:
Central European Journal of Energetic Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The Influence of Cycle Sputtering Deposition on the Properties of Cu₂ZnSnS₄ Thin Films
Autorzy:
Sun, Y.
Yao, B.
Long, D.
Meng, X.
Liu, L.
Hua, Z.
Powiązania:
https://bibliotekanauki.pl/articles/1398930.pdf
Data publikacji:
2016-06
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
78.40.Fy
Opis:
Three kinds of precursor thin films with stacking orders of Cu/Sn/ZnS, Cu/Sn/ZnS/Cu/Sn/ZnS and Cu/Sn/ZnS/Cu/Sn/ZnS/Cu/Sn/ZnS were prepared by magnetron sputtering and annealed with sulfur powder. The microstructure, morphology and optical properties of thin films were investigated by X-ray diffraction, the Raman scattering, scanning electron microscopy and UV-visible spectrophotometer. The increase of cycle number decreases the sulfurizing temperature of the formation of Cu₂ZnSnS₄ phase. Chemical composition can be controlled by cycle sputtering deposition. After sulfurizing at 500°C, the particle size and the band gap increase with increasing cycle number.
Źródło:
Acta Physica Polonica A; 2016, 129, 6; 1169-1172
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 50

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