- Tytuł:
- Effect of natural phenols on the catalytic activity of cytochrome P450 2E1
- Autorzy:
-
Mikstacka, Renata
Gnojkowski, Jerzy
Baer-Dubowska, Wanda - Powiązania:
- https://bibliotekanauki.pl/articles/1043696.pdf
- Data publikacji:
- 2002
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
resveratrol
tannic acid
polyphenols
inhibition kinetics
mechanism-based inhibition
CYP2E1
protocatechuic acid - Opis:
- The effect of protocatechuic acid, tannic acid and trans-resveratrol on the activity of p-nitrophenol hydroxylase (PNPH), an enzymatic marker of CYP2E1, was examined in liver microsomes from acetone induced mice. trans-Resveratrol was found to be the most potent inhibitor (IC50 = 18.5 ± 0.4 mM) of PNPH, while protocatechuic acid had no effect on the enzyme activity. Tannic acid with IC50 = 29.6 ± 3.3 mM showed mixed- and trans-resveratrol competitive inhibition kinetics (Ki = 1 mM and 2.1 mM, respectively). Moreover, trans-resveratrol produced a NADPH-dependent loss of PNPH activity, suggesting mechanism-based CYP2E1 inactivation. These results indicate that trans-resveratrol and tannic acid may modulate cytochrome P450 2E1 and influence the metabolic activation of xenobiotics mediated by this P450 isoform.
- Źródło:
-
Acta Biochimica Polonica; 2002, 49, 4; 917-925
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł