- Tytuł:
- First Report on Fusarium brachygibbosum Isolate FIR 16_ITS Isolated from Iraqi Wheat Plant
- Autorzy:
-
Ali, Hamdia Z.
Hameed, Mahdi S.
Abdulrahman, Abdulrahman A.
Saood, Hutham M. - Powiązania:
- https://bibliotekanauki.pl/articles/123408.pdf
- Data publikacji:
- 2020
- Wydawca:
- Polskie Towarzystwo Inżynierii Ekologicznej
- Tematy:
-
phylogenetic analysis
rDNA
Iraq - Opis:
- In this study, the Fusarium Brachygibbosum fungus isolate FIR 16_ITS was isolated for the first time from the infected wheat plants in Iraq, during the winter of 2018, in the AL-Tewatha region – 20 km south east of Baghdad. In order to verify the identity of the species of the F. brachygibbosum isolate FIR 16_ITS with the growth symptoms on the wheat plants, the wheat seeds and tissue plants were obtained and used as a source for the re-isolation of the infection agent. F. brachygibbosum isolate FIR 16_ITS was hardly identified using visual approach. However, a molecular technique is important in verifying the species of F. brachygibbosum isolate FIR 16_ITS. The isolate obtained was used for the extraction of DNA and later used for the molecular identification and phylogenetic analyses based on rDNA-internal transcribed region (ITS) primer sets. The authors successfully obtained the amplified products of the ITS-rDNA region, and a sequence analysis indicated that the Fusarium sp. isolate FIR 16_ITS species screened in this study belonged to the F. brachygibbosum species. It was recorded in the NCBI under the following information NCBI; BioSample; Accession no.; SAMN11408139; ID: 11408139 and NCBI; BioProject; Accession no.; PRJNA532637; ID: 679434 (Locus Tag Prefix; E7750).
- Źródło:
-
Journal of Ecological Engineering; 2020, 21, 3; 81-86
2299-8993 - Pojawia się w:
- Journal of Ecological Engineering
- Dostawca treści:
- Biblioteka Nauki
Artykuł