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Tytuł:
Handling of endoscopic equipment after use in the case of a patient with suspected prion disease
Autorzy:
Gruszecka, J.
Filip, R.
Powiązania:
https://bibliotekanauki.pl/articles/2085936.pdf
Data publikacji:
2020
Wydawca:
Instytut Medycyny Wsi
Tematy:
infections
prion protein
decontamination
Opis:
Introduction. Prion diseases are slow-acting, neurodegenerative diseases found in humans and many species of animals. Although they occur very rarely in humans, currently, an increase in this type of disease is being observed, probably as a result of exposure to infectious prions causing BSE disease in cows. Objective. The aim of the procedures described in the article is to minimize the risk of human-to-human transfer of all forms of transmissible spongiform encephalopathy, including variant CJD (vCJD) by contaminated medical equipment. Brief description of the state of knowledge. All diseases caused by prions, referred to as transmissible spongiform encephalopathies, are fatal. They are characterized by a long development period (up to several decades). Enormous problems are connected with the risk of transferring prions from patient to patient on the surface of instruments used in medical procedures. Laboratory tests indicate that standard disinfection and sterilization procedures may be insufficient to completely remove infectious proteins from contaminated instruments. One of the methods of infection prevention involves taking equipment used for surgery within the brain, tonsils or appendix, into quarantine until biopsy results of these organs have been received that exclude, as far as possible, asymptomatic carriage of prions. Conclusions. Whenever possible and justified, disposable-use instruments should be used for invasive surgery in patients with definite, clinically probable cases of CJD (vCJD). After use, these instruments should be incinerated.
Źródło:
Annals of Agricultural and Environmental Medicine; 2020, 27, 3; 319-325
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The role of bronchoscopy in the diagnosis of lower respiratory tract infections
Autorzy:
Mierzejewski, Michał
Krenke, Rafał
Powiązania:
https://bibliotekanauki.pl/articles/1401689.pdf
Data publikacji:
2016
Wydawca:
Index Copernicus International
Tematy:
bronchoscopy
lower respiratory tract infections
bronchoalveolar lavage
opportunistic infections
tuberculosis
pulmonary mycoses
Opis:
Bronchoscopy was introduced into clinical practice over 100 years ago. Due to technological advances, diagnostic capabilities of current bronchoscopy are not limited to the trachea and proximal bronchi but also include the peripheral airways as well as various anatomical structures located outside the bronchi. A wide range of available techniques that include bronchoalveolar lavage, protected microbiological brush, transbronchial biopsy of the lungs and mediastinal lymph nodes makes bronchoscopy useful in diagnosing various lower respiratory tract infections. For example, the collection of high quality biological samples for microbiological examination plays a crucial role when treating patients with nosocomial pneumonia. Bronchoscopy may provide the samples directly from the site of infection. In immunocompromised hosts bronchoscopy is routinely used as the diagnostic tool in lower respiratory tract infections. Due to implementation of novel therapies, the number of immunocompromised patients is steadily increasing, hence there are growing needs for effective diagnosis of opportunistic pulmonary infections. In the specific group of lung transplant recipients, bronchoscopy play a crucial role in monitoring the rejection process and also in differentiating between the rejection and pulmonary infections. Bronchoscopy is also useful in diagnosing tuberculosis or nontuberculous pulmonary infections. This particularly refers to patients who are unable to produce sputum for microbiological examination.
Źródło:
Polski Przegląd Otorynolaryngologiczny; 2016, 5, 3; 1-6
2084-5308
2300-7338
Pojawia się w:
Polski Przegląd Otorynolaryngologiczny
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Does the use of chlorhexidine gel reduce the frequency of surgical site infections?
Autorzy:
Kolasiński, Wojciech
Pomorski, Lech
Powiązania:
https://bibliotekanauki.pl/articles/1391686.pdf
Data publikacji:
2020
Wydawca:
Index Copernicus International
Tematy:
surgical site infections
chlorhexidine
Opis:
Introduction: Surgical site infections (SSI) involve 2–11% of all surgical procedures. Paper assumption: The use of 6% gel with chlorhexidine as an element of preoperative skin preparation of the operated area reduces the number of surgical site infections. Aim: The aim of the study was to assess the impact of body mass index (BMI), neutrophil to lymphocyte ratio (NLR), total protein, glucose, length of hospitalization before surgery, duration of surgery, length of drainage maintenance, transfusion of red blood concentrate on the number of SSI. Materials and methods: 248 patients were subjected to prospective analysis. Patients were operated at the Department of General and Oncological Surgery of the Provincial Specialist Hospital in Zgierz. Patients were divided into three groups depending on the microbiological degree of cleanliness of the postoperative wound: Group I – clean wounds, Group II – cleancontaminated wounds, Group III – contaminated wounds, which also included emergency surgerical procedures. In each group two subgroups were distinguished depending on the method of preoperative preparation of the surgical field: A – gel without CHG, B – 6% gel with CHG. Results: Surgical site infections were found in 22 patients (8.9%). The respective frequencies for groups I, II, III are: 3.0% vs 12.9% vs 12.7%. An increase in NLR by one unit resulted in a higher incidence of surgical site infections by 11%. A transfusion of RBC to the patient resulted in a 3.5-fold increase in the frequency of surgical site infections. Extending the drain maintenance time by one day increases the SSI frequency by 41%. Lowering the total protein concentration by at least 1 g/dl below normal increases the risk of surgical site infections almost three times. Conclusions: The use of a 6% gel preparation with chlorhexidine as an element of preoperative preparation of the surgical field reduces the risk of surgical site infections, especially in clean-contaminated and contaminated wounds.
Źródło:
Polish Journal of Surgery; 2020, 92, 3; 26-31
0032-373X
2299-2847
Pojawia się w:
Polish Journal of Surgery
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Utilisation of peptides against microbial infections – a review
Autorzy:
Mirski, T.
Niemcewicz, M.
Bartoszcze, M.
Gryko, R.
Michalski, A.
Powiązania:
https://bibliotekanauki.pl/articles/2081601.pdf
Data publikacji:
2018
Wydawca:
Instytut Medycyny Wsi
Tematy:
antimicrobial peptides
infections
drugs
therapy
Opis:
The emergence of resistance in microorganisms on a global scale has made it necessary to search for new antimicrobial factors. Antimicrobial peptides (AMPs) seem to meet these expectations. AMPs are produced by bacteria, viruses, plants, and animals, and may be considered as a new class of drugs intended for the prophylaxis and treatment of both systemic and topical infections. The aim of this study is to review the results of studies on the use of peptides to combat infections in vivo. Antimicrobial peptides may be applied topically and systemically. Among the peptides used topically, a very important area for their application is ophthalmology. AMPs in ophthalmology may be used mainly for the protection of contact lenses from ocular pathogens. Many AMPs are in clinical trials for application in the therapy of local infections. There may be mentioned such preparations as: pexiganan (magainin analogue), MX-226 (based on indolicidin), NEUPREX (isolated from human BPI (bactericidal/permeability-increasing) protein), IB-367 (variant of porcine protegrin), P113 (based on histatin), daptomycin, polymyxins, as well as peptidomimetics. In the combat against systemic infections are used such peptides as: P113D (modified P113 peptide containing D-amino acids), colistin, peptoids, and peptides containing non-typical amino acids or non-peptide elements. AMPs are also used as antiprotozoal, antifungal, antitoxic and immunostimulatory agents. The limitations in the use of peptides in the treatment of infections, such as susceptibility to proteolysis, and resistance of microorganisms to the peptides, are also discussed. AMPs are a promising strategy in the fight against microbial infections.
Źródło:
Annals of Agricultural and Environmental Medicine; 2018, 25, 2; 205-210
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Evaluation of clinical usefulness of serum neopterin determination in children with bacterial infections
Autorzy:
Plata-Nazar, Katarzyna
Luczak, Grażyna
Liberek, Anna
Dudzinska-Gehrmann, Julita
Sznurkowska, Katarzyna
Landowski, Piotr
Kaminska, Barbara
Powiązania:
https://bibliotekanauki.pl/articles/1039150.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
neopterin
children
urinary tract infections
Opis:
Neopterin (NPT) (6-D-erythro-trihydroxypropyl pteridin) is one of the indicators of the immune system activity. Elevated neopterin concentration occurs in diseases mostly involving stimulation of cellular immunity. The determination of neopterin concentration, usually in blood serum and urine but also in many other bodily fluids, has already been applied in many areas of medicine, such as transfusiology, transplantology, oncology, infectious diseases and autoimmunological diseases. Objective. The aim of this work is to evaluate clinical usefulness of serum neopterin determination in children with urinary tract infections of confirmed bacterial etiology. Material. The study involved 56 children with bacterial urinary tract infections - patients of the Clinic of Paediatrics, Paediatric Gastroenterology, Hepatology & Paediatric Nutrition of Medical University of Gdańsk in the years 2012-2013. The control group included 105 healthy children. Results. The values of NPT concentration in blood serum obtained in the group of children with urinary tract infections did not significantly differ from the values obtained in the control group. Conclusions. The determination of neopterin concentration in children with bacterial urinary tract infections is not a clinically useful parameter.
Źródło:
Acta Biochimica Polonica; 2015, 62, 1; 133-137
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Electron work function as a direct parameter for bacterial infection risk of implant surfaces
Autorzy:
Gołda-Cępa, M.
Syrek, K.
Brzychczy-Włoch, M.
Sulka, G. D.
Kotarba, A.
Powiązania:
https://bibliotekanauki.pl/articles/284984.pdf
Data publikacji:
2016
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
bacterial infections
implants
nanoporous materials
Źródło:
Engineering of Biomaterials; 2016, 19, 138; 58
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Analiza wstępowania szpitalnych zakażeń układu moczowego w 10-letniej obserwacji oddziału chorób wewnętrznych i nefrologii w Małopolsce
Autorzy:
Wałaszek, Marta
Kołpa, Małgorzata
Wolak, Zdzisław
Sydor, Antoni
Pelczar, Anna
Powiązania:
https://bibliotekanauki.pl/articles/2097675.pdf
Data publikacji:
2017-06-30
Wydawca:
Państwowa Wyższa Szkoła Zawodowa w Tarnowie
Tematy:
ddział chorób wewnętrznych
zakażenie szpitalne
Healthcare – Associated Infections (HAI)
szpitalne zakażenie układu moczowego (UTI)
E. coli
P. aeruginosa
internal medicine department
hospital-acquired infections
healthcare associated infections
urinary tract infections
Opis:
Wstęp: Zakażenia szpitalne są jednym z poważniejszych zagrożeń zdrowia pacjenta podczas hospitalizacji. Wśród nich zakażenia szpitalne HAI (Healthcare – Associated Infections). Najczęściej występującą formą są szpitalne zakażenia układu moczowego (UTI – Urinary Tract Infection). Cel: W celu zbadania częstości występowania szpitalnych zakażeń układu moczowego poddano analizie strukturę zakażeń szpitalnych występujących u pacjentów hospitalizowanych w Oddziale Chorób Wewnętrznych i Nefrologii Szpitala Wojewódzkiego im. Św. Łukasza w Tarnowie. Materiał i metody: Analizowano dane dotyczące 13 965 pacjentów hospitalizowanych w Oddziale Chorób Wewnętrznych i Nefrologii w latach od 2006 do 2015. W analizie materiału wykorzystano standardowe metody epidemiologiczne i ujednolicone definicje zakażeń szpitalnych wydane przez ECDC (European Center for Disease Prevention and Control) oraz CDC (Centers for Disease Control and Prevention). Wyniki: Wykryto 237 szpitalnych UTI co stanowiło 33% wszystkich zakażeń szpitalnych wykrytych w badanym oddziale. Zachorowalność wynosiła 1,7%, w tym: 1,5% dla potwierdzonych mikrobiologicznie zakażeń (UTI-A: Microbiologically Confirmed Symptomatic UTI) i 0,2% bez potwierdzenia mikrobiologicznego (UTI-B: Not Microbiologically Confirmed Symptomatic UTI). Współczynnik gęstości zachorowań na 1000 osobodni pobytu (ID – density incidence /1000 osobodni pobytu) wynosił 0,2/1000. Rozpoznano 168 przypadków zakażeń UTI związanych z cewnikiem moczowym i 69 przypadków bez cewnika moczowego. Współczynnik gęstości zachorowań dla UTI z cewnikiem moczowym wynosił 3,3 na 1000 osobodni z cewnikiem moczowym. Wśród czynników etiologicznych, które izolowano z materiałów pochodzących od pacjentów z zakażeniem układu moczowego (UTI) dominowały: Escherichia coli 63 (29%), Enterococcus spp. 37 (16%), Klebsiella spp. 23 (11%). Wnioski: Dziesięcioletnia obserwacja szpitalnych UTI występujących w oddziale chorób wewnętrznych i nefrologii wykazała możliwość przeprowadzenia bardzo dokładnej analizy epidemiologicznej tych zakażeń. Porównanie gęstości występowania szpitalnych UTI uzyskane w badanym oddziale z odnotowaną w programach CDC pozwala wnioskować, iż sytuacja epidemiologiczna w badanym oddziale nie odbiega znacząco od sytuacji w innych krajach.
Introduction: Hospital-acquired infections are one of the most serious health threats during a patient’s stay in hospital, including healthcare associated infections (HAI). The most typical form of hospital-acquired infections is urinary tract infection (UTI). Objective: To examine the frequency of appearing UTIs, the structure of UTIs in in-patients in the department of internal medicine and nephrology at Saint Lucas’s general hospital in Tarnów was analysed. Materials and methods: Data analysis of 13 965 in-patients staying in the department of internal medicine and nephrology from 2006 to 2015 was carried out. To investigate these data epidemiological methods and standard definitions of hospital- acquired infections issued by European Center for Disease Prevention as well as Control and Centers for Disease Control and Prevention were used. Results: 237 hospital-acquired UTIs were revealed, which is 33% of all UTIs revealed in the investigated ward. The UTI incidence rate was 1.7% including 1.5% for microbiologically confirmed symptomatic UTIs and 0.2% not microbiologically confirmed symptomatic UTIs. The incidence density rate per 1 000 person-days was 0.2 over 1 000. The number of revealed catheter-related cases was 168, and not catheter-related cases – 69. The incidence density rate of UTIs associated with urinary catheters was 3.3 per 1 000 person-days. The dominant etiological factors, which were taken to be detected from the infected patients’ specimens, were: Escherichia coli 63 (29%), Enterococcus spp. 37(16%), Klebsiella spp. 23 (11%). Conclusions: A 10 year observation of UTIs, which have appeared in the department of internal medicine and nephrology, allowed to conduct the accurate analysis of these infections. The comparison of urinary tract infection rates done in the investigated ward, and recorded in the Research Participation Programs at the Centers for Disease Control and Prevention (CDC), allows to draw the conclusion that the presented epidemiological situation does not differ significantly from other countries.
Źródło:
Health Promotion & Physical Activity; 2017, 2, 3; 141-154
2544-9117
Pojawia się w:
Health Promotion & Physical Activity
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
PCR-based detection of Helicobacter pylori and non-Helicobacter pylori species among humans and animals with potential for zoonotic infections
Autorzy:
Youssef, A.I.
Afifi, A.
Abbadi, S.
Hamed, A.
Enany, M.
Powiązania:
https://bibliotekanauki.pl/articles/2087111.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Helicobacter spp.
zoonotic infections
detection
identification
Źródło:
Polish Journal of Veterinary Sciences; 2021, 24, 3; 445-450
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Students’ attitudes to tick risks
Autorzy:
Nejezchlebová, Helena
Kiewra, Dorota
Žákovska, Alena
Ovesná, Petra
Powiązania:
https://bibliotekanauki.pl/articles/990882.pdf
Data publikacji:
2016
Wydawca:
Instytut Medycyny Wsi
Tematy:
questionnaire
tick-borne infections
knowledge
prevention
Opis:
Introduction and objectives. The ever-increasing number of patients with tick-borne diseases resulted in the presented study investigating the awareness, attitudes and knowledge among students about the threats arising from tick bites and preventive anti-tick practices. Materials and method. Questionnaires concerning these issues were distributed amongst Czech and Polish university students of science. Responses were analyzed by nationality and by gender. Results. Nearly all respondents were aware of the risks arising from ticks and could name at least one disease transmitted by ticks. The Czech students felt more threatened by tick-borne diseases, had more frequently suffered from Lyme borreliosis and were vaccinated against tick-borne encephalitis more often than the Polish students. A large number of the participants applied preventive measures against ticks in order to protect themselves. The Czech students believed in the effectiveness of repellents statistically more often than the Polish students, while effectiveness is the main criterion for selection of the right repellent in both groups. Conclusion. Differences in preferences between the two nations appeared in many areas, e.g. the Czechs felt more threatened by all kind of risks and suffered from Lyme disease more frequently. Gaps can still be found in both the knowledge and behaviour among the respondents. It can be expected that the general public knowledge of this issue is rather limited in comparison with the students participating in the study, who are systematically educated in the field.
Źródło:
Annals of Agricultural and Environmental Medicine; 2016, 23, 3
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Acinetobacter Baumannii Nosocomial Infections
Autorzy:
Sieniawski, Karol
Kaczka, Krzysztof
Rucińska, Monika
Gagis, Ludmiła
Pomorski, Lech
Powiązania:
https://bibliotekanauki.pl/articles/1396395.pdf
Data publikacji:
2013-09-01
Wydawca:
Index Copernicus International
Tematy:
Acinetobacter baumannii
nosocomial infections
multidrug-resistant
Opis:
Nosocomial infections caused by strains Acinetobacter baumannii strands are a growing clinical problem. The occurrence of multidrug-resistant strands is observed and that limits the ways of therapy considerably. The aim of the study was to determine the rate of infection and susceptibility spectrum of the species Acinetobacter baumannii isolated from patients treated at Maria Skłodowska-Curie Memorial Hospital in Zgierz with particular emphasis on surgical wards. Materials and methods. The material consisted of Acinetobacter baumannii isolates were obtained from samples of materials from patients treated at Maria Skłodowska-Curie Memorial Hospital in Zgierz from January to December 2011. Isolated bacterial strains were cultured at microbiological substrates. Isolates were identified to species using the VITEK 2 GN card (bioMérieux) and Vitek 2 automated system (bioMérieux). Susceptibility towards antibiotics of particular strains was determined by the means of AST NO 93 card. In the case of resistance towards carbapenem, the MIC was marked by E-test with Mueller Hinton substrate. The occurrence of MBL was verified by the means of disc system with Mueller Hinton substrate. Results. We have shown that total number of Acinetobacter baumannii infections at hospital was 140 (10,31% of total results of cultures). Percentage of Acinetobacter baumannii infections at wards: Intensive Care Unit 48%, Surgical Departments 20%, Internal Diseases Department 16%, Neurology 13%, other wards - 3%. The susceptibility percentage of Acinetobacter Baumannii against antibiotics: colistin 90%, imipenem 64%, meropenem 43%, ampicillin-sulbactam 28%, amikacin 27%, gentamicin 24%, cefepime 9%, ceftazidime 7%, ciprofloxacin 7% Conclusions. Acinetobacter baumannii infections are a significant proportion of nosocomial infections. Most relate to surgical wards and ICUs. Acinetobacter baumannii is resistant against most antibiotics. The highest percentage of sensitivity demonstrated for colistin and carbapenems
Źródło:
Polish Journal of Surgery; 2013, 85, 9; 483-490
0032-373X
2299-2847
Pojawia się w:
Polish Journal of Surgery
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Microbiological assessment of cleanliness of surfaces and equipment in a children’s operating theatre on the example of a selected hospital
Autorzy:
Gruszecka, J.
Gutkowska, D.
Filip, R.
Powiązania:
https://bibliotekanauki.pl/articles/2083138.pdf
Data publikacji:
2019
Wydawca:
Instytut Medycyny Wsi
Tematy:
nosocomial infections
pathogenic microorganisms
microbiological cleanliness
Opis:
Introduction. The operating theatre is one of the most important places in a hospital. Due to the presence of numerous reservoirs of microorganisms and the invasiveness of surgical procedures it is necessary to ensure high hygiene standards in these locations. Objective. The aim of the study was to carry out a qualitative assessment of the microbiological cleanliness of the surfaces and equipment in an operating theatre. Materials and method. The results of microbiological tests of the surfaces and equipment of the Children’s Operating Theatre in Clinical Provincial Hospital No. 2 in Rzeszów, southeast Poland, during 2007–2012 were reviewed retrospectively. Results and conclusions. For the analysis, a total of 1,819 swabs were collected, of which 1.05% were positive. Positive results were obtained mainly from samples taken from moist places (57.9%). Among the microorganisms isolated, Gram- negative bacteria constituted the majority (57.9%), Pseudomonas bacteria were found most frequently (31,6%). Isolated microbes can be the etiological agent of nosocomial infections.
Źródło:
Annals of Agricultural and Environmental Medicine; 2019, 26, 2; 249-251
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Incidence of smell and taste disorders and associated factors in patients with mild to moderate COVID-19
Autorzy:
Guillén Martínez, Alberto
Andreu Gálvez, Marina
Rodríguez Sanz, Sara
Hernández Ruiz, Paula
García Morillas, Antonio
Esteban Sánchez, Tomás
Powiązania:
https://bibliotekanauki.pl/articles/23203216.pdf
Data publikacji:
2020-08-06
Wydawca:
Index Copernicus International
Tematy:
ageusia
anosmia
coronavirus infections
hyposmia
olfaction disorders
Opis:
Objectives: To determine the incidence of smell and taste disorders in our health department and to analyse the factors that could be associated with these symptoms in patients with COVID-19. Methods: We conducted an observational descriptive study of all patients with COVID-19 in our health area diagnosed between 2020/03/10 and 2020/04/14. Factors related to smell and taste disorders were analysed. Results: A total of 126 patients, 63 women and 63 men, aged 16–80 years, were included. As many as 69 patients (62.7%) presented hyposmia, and 58 (46%) of them had anosmia. A total of 75 patients (59.5%) presented hypogeusia, and 57 (45.2%) of them had ageusia. The risk factors that were most commonly associated with these disorders were the female sex (adjusted odds ratio, aOR 2.43 for smell disorders and 2.44 for taste disorders), allergic rhinitis (aOR 3.34 for smell disorders) and a younger age. A protective factor was arterial hypertension (aOR 0.51 for smell disorders and 0.35 for taste disorders). A history of tonsillectomy was the risk factor for taste disorder (aOR 5.23). Conclusion: Our results indicate that these sensory disorders occurred more frequently in female patients and in young patients with mild to moderate COVID-19 infection who progressed with mild nasal congestion, posterior rhinorrhoea and without anterior rhinorrhoea. The recovery of taste occurred before the recovery of smell.
Źródło:
Polish Journal of Otolaryngology; 2021, 75, 5; 31-38
0030-6657
2300-8423
Pojawia się w:
Polish Journal of Otolaryngology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Cross-infections with Pseudomonas aeruginosa in patients with cystic fibrosis attending the Warsaw Centre
Zakażenia krzyżowe Pseudomonas aeruginosa u pacjentów z mukowiscydozą pozostających pod opieką Instytutu Matki i Dziecka
Autorzy:
Milczewska, Justyna
Wołkowicz, Tomasz
Zacharczuk, Katarzyna
Kwiatkowska, Monika
Sands, Dorota
Powiązania:
https://bibliotekanauki.pl/articles/1032970.pdf
Data publikacji:
2015
Wydawca:
Wydawnictwo ALUNA
Tematy:
PFGE
Pseudomonas aeruginosa
cross-infections
cystic fibrosis
Opis:
Aim: 1. To assess the prevalence of cross-infections with P. aeruginosa in order to evaluate the epidemiological situation of this infection in patients with cystic fibrosis attending our centre; 2. To correlate the clinical features of the patients carrying a potentially transmissible strain with the entire study group in order to determine the risk factors and possible effects of its acquisition. Material and methods: 170 Pseudomonas aeruginosa strains obtained from the respiratory tract of 75 cystic fibrosis patients attending the Warsaw Centre in 2011 and 2012 were typed using restriction enzyme analysis-pulsed field gel electrophoresis (Spe I restriction enzyme was used). Simultaneously, the information concerning contacts between patients, as well as several clinical data regarding the course of the disease were collected. Results: Twenty four clusters of strains were detected. The main cluster included 49 isolates derived from 21 patients. The other detected clusters included 2 to 12 isolates derived from 1 to 7 patients. Three clusters comprised the isolates derived from three pairs of siblings. There were 15 clusters containing 2 to 7 strains belonging to the same patient. The remaining 24 patients were infected with their own strains, not fitting any clonal group. Several clinical parameters showed that the 21 patients whose strains constituted the main cluster, were in worse clinical condition than the other patients in the study group. Moreover, the total duration of their hospitalizations in order to perform intravenous antibiotic treatment was longer. Conclusions: 1. Frequent hospitalizations of CF patients with a more severe course of the disease seem to be a risk factor of cross-infections with P. aeruginosa. 2. Intensification of measures to prevent cross-infection, such as hygienic precautions, patient segregation, introduction of home intravenous antibiotic therapy programme, as well as further education of patients and their parents should lead to the improvement of the epidemiological situation in our centre.
Cel: 1. Ocena częstości występowania zakażeń krzyżowych Pseudomonas aeruginosa i ich możliwych konsekwencji klinicznych u pacjentów chorujących na mukowiscydozę leczonych w naszym ośrodku; 2. Porównanie cech klinicznych grupy pacjentów, u których doszło do zakażenia krzyżowego z cechami klinicznymi całej grupy badanej, w celu określenia czynników ryzyka i możliwych skutków takiego zakażenia. Materiał i metody: 170 szczepów Pseudomonas aeruginosa wyizolowanych w latach 2011-2012 z dróg oddechowych 75 pacjentów z mukowiscydozą pozostających pod opieką Instytutu Matki i Dziecka typowano metodą makrogenomowej analizy restrykcyjnej z elektroforezą w zmiennym polu elektrycznym (z wykorzystaniem endonukleazy Spe I). Zbierano wywiady dotyczące najważniejszych parametrów klinicznych, a także kontaktów pomiędzy pacjentami. Wyniki: W toku analizy wyodrębniono 24 grupy klonalne. Największa grupa zawierała 49 izolatów pochodzących od 21 chorych. Inne grupy obejmowały od 2 do 12 izolatów pochodzących od 1 do 7 pacjentów. Trzy grupy zawierały szczepy pochodzące od trzech rodzeństw. Piętnaście grup klonalnych obejmowało od 2 do 7 szczepów należących do tego samego pacjenta. Pozostałych 24 pacjentów było zakażonych szczepami, nie należącymi do żadnej grupy klonalnej. Ocena parametrów klinicznych wykazała, że przebieg choroby u 21 pacjentów, których szczepy stanowiły największą grupę klonalną, był cięższy niż u pozostałych pacjentów w grupie badanej. Także łączny czas trwania ich hospitalizacji (antybiotykoterapii dożylnych) był dłuższy. Wnioski: 1. Częste hospitalizacje pacjentów z mukowiscydozą w gorszym stanie klinicznym wydają się być istotnym czynnikiem ryzyka zakażeń krzyżowych Pseudomonas aeruginosa. 2. Intensyfikacja działań zapobiegających zakażeniom krzyżowym, takich jak środki higieny, izolacja chorych, wprowadzenie w Polsce programu domowej antybiotykoterapii dożylnej, a także dalsza edukacja pacjentów i ich rodziców odnośnie dróg transmisji zakażeń bakteryjnych, prawdopodobnie przyczynią się do poprawy sytuacji epidemiologicznej w naszym ośrodku.
Źródło:
Developmental Period Medicine; 2015, 1; 60-65
1428-345X
Pojawia się w:
Developmental Period Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Factors affecting decision concerning influenza vaccination among students of medical faculties
Autorzy:
Woźniak-Kosek, Agnieszka
Kempińska-Mirosławska, Bogumiła
Mendrycka, Mariola
Saracen, Agnieszka
Hoser, Grażyna
Powiązania:
https://bibliotekanauki.pl/articles/1039225.pdf
Data publikacji:
2014
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
influenza
vaccination against influenza- knowledge
infections
students
Opis:
Influenza is one of the most common cyclic respiratory diseases in humans. Methods of prevention are multidirectional, but the most effective and most efficacious way to prevent influenza and its complications is through preventive vaccination. This work aims to determine different factors affecting the decision concerning influenza vaccine. The percentage of people vaccinated against the flu was evaluated, as well as their knowledge of post-influenza complications, etc. among full-time students and bridging studies of nursing and physiotherapy (full-time and part-time) at the University of Technology and Life Sciences in Radom, and students of medicine and pharmacy at the Medical University of Łódź. The research tool was the authors' questionnaire with 18 questions. The surveys conducted, consisting of multiple choice questions, were anonymous. In total, the survey involved 470 students. Overall, the number of people who were vaccinated against influenza in the 2012/13 epidemic season numbered 15 respondents, representing 5.84% of the total group of respondents. For the group of nursing students it was 6%, for physiotherapy students 5%, for students of medicine and pharmacy 14%. The percentage of respondents who said they would get vaccinated if the vaccinaton was free of charge was also low. Increasing the percentage of people vaccinated against influenza (immunization coverage) is a very important measure in preventing influenza epidemics. Therefore, it is necessary to identify the reasons why people are reluctant to be vaccinated against influenza, particularly among students who will work in the future in the health care services sector.
Źródło:
Acta Biochimica Polonica; 2014, 61, 4; 829-832
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Analysis of uropathogenic Escherichia coli biofilm formation under different growth conditions
Autorzy:
Adamus-Białek, Wioletta
Kubiak, Anna
Czerwonka, Grzegorz
Powiązania:
https://bibliotekanauki.pl/articles/1038911.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
biofilm
uropathogenic Escherichia coli
urinary tract infections
Opis:
The ability to form different types of biofilm enables bacteria to survive in a harsh or toxic environment. Different structures of biofilms are related to different surfaces and environment of bacterial growth. The aim of this study was analysis of the biofilm formation of 115 clinical uropathogenic Escherichia coli strains under different growth conditions: surface for biofilm formation, medium composition and time of incubation. The biofilm formation after 24 h, 48 h, 72 h and 96 h was determined spectrophotometrically (A531) after crystal violet staining and it was correlated with bacterial growth (A600). The live and dead cells in biofilm structures was also observed on the glass surface by an epi-fluorescence microscope. Additionally, the presence of rpoS, sdiA and rscA genes was analyzed. The statistical significance was estimated by paired T-test. The observed biofilms were different for each particular strain. The biofilm formation was the highest in the rich medium (LB) after 24 h and its level hasn't changed in time. When biofilm level was compared to bacterial growth (relative biofilm) - it was higher in a minimal medium in comparison to enriched medium. These results suggest that most of the bacterial cells prefer to live in a biofilm community under the difficult environmental conditions. Moreover, biofilm formation on polyurethane surface did not correlate with biofilm formation on glass. It suggests that mechanisms of biofilm formation can be correlated with other bacterial properties. This phenomenon may explain different types of biofilm formation among one species and even one pathotype - uropathogenic Escherichia coli.
Źródło:
Acta Biochimica Polonica; 2015, 62, 4; 765-771
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 150

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