- Tytuł:
- Imaging of hypoxia in small animals with 18F fluoromisonidasole
- Autorzy:
-
Kilian, K.
Rogulski, Z.
Cheda, Ł.
Drzał, A.
Gerszewska, M.
Cudny, M.
Elas, M. - Powiązania:
- https://bibliotekanauki.pl/articles/148618.pdf
- Data publikacji:
- 2016
- Wydawca:
- Instytut Chemii i Techniki Jądrowej
- Tematy:
-
18F-fluoromisonidazole
radiopharmaceuticals
hypoxia imaging - Opis:
- A method of automated synthesis of [18F]fluoromisonidazole ([18F]FMISO) for application in preclinical studies on small animals was presented. A remote-controlled synthesizer Synthra RNplus was used for nucleophilic substitution of NITTP (1-(2'-nitro-1'-imidazolyl)-2-O-tetrahydropyranyl-3-O-toluenesulfonyl-propanediol) with 18F anion. Labeling of 5 mg of precursor was performed in anhydrous acetonitrile at 100°C for 10 min, and the hydrolysis with HCl was performed at 100°C for 5 min. Final purifi cation was done with high-performance liquid chromatography (HPLC) and the radiochemical purity of radiotracer was higher than 99%. Proposed [18F]FMISO synthesis was used as a reliable tool in studies on hypoxia in Lewis lung carcinoma (LLC) in mouse models.
- Źródło:
-
Nukleonika; 2016, 61, 2; 119-223
0029-5922
1508-5791 - Pojawia się w:
- Nukleonika
- Dostawca treści:
- Biblioteka Nauki
Artykuł