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    Wyświetlanie 1-6 z 6
    Tytuł:
    Characterizations and evaluations on the bonding quality of molybdenum disilicides
    Charakterystyka i ocena jakości połączeń wytworzonych przez MoSi2
    Autorzy:
    Lu, L. W.
    Qin, W. G.
    Powiązania:
    https://bibliotekanauki.pl/articles/352922.pdf
    Data publikacji:
    2011
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    MoSi2
    stal nierdzewna 316L
    spiekanie
    316L stainless steel
    sintering
    Opis:
    In this study, we proposed a new method using the spark plasma sintering technique to bond ceramics to alloys. MoSi2 and 316L stainless steel were chosen as sample materials and can be welded well with graded interlayers. We found that dense uniformed bondings were achieved because of the comparable coefficient of thermal expansion of the interlayers. Furthermore, such a compatibility between the graded interlayers prevented MoSi2 with low toughness from the occurrence of microcracks resulted from the residual stresses formed during cooling of the bondings.
    W pracy zaproponowano nowa metodę łączenia ceramiki i stopów metodą spiekania iskrowo plazmowego SPS (spark plasma sintering). MoSi2 i stal nierdzewna 316L zostały wybrane jako przykładowe materiały i moga byc łaczone poprzez gradientowe warstwy pośrednie. Okazało się, że zwarte, jednorodne połączenia zostały uzyskane dzieki porównywalnemu współczynnikowi rozszerzalności cieplnej warstw pośrednich. Ponadto, tego rodzaju zgodnosc pomiedzy warstwami pośrednimi zapobiegła wystapieniu mikropęknięć w MoSi2, który jest materiałem o niskiej twardości, będacych wynikiem naprezen powstałych podczas chłodzenia połączeń.
    Źródło:
    Archives of Metallurgy and Materials; 2011, 56, 4; 1271-1271
    1733-3490
    Pojawia się w:
    Archives of Metallurgy and Materials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Differential expression of Toll - like receptor 4 signaling pathway genes in Escherichia coli F18 - resistant and - sensitive Meishan piglets
    Autorzy:
    Liu, Y.
    Gan, L.N.
    Qin, W.Y.
    Sun, S.Y.
    Zhu, G.Q.
    Wu, S.L.
    Bao, W.B.
    Powiązania:
    https://bibliotekanauki.pl/articles/31946.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    The Toll-like receptor 4 (TLR4) signaling pathway is an important inflammatory pathways associated with the progression of numerous diseases. The aim of the present study was to investigate the relationship between TLR4 signaling and resistance to Escherichia coli F18 in locally weaned Meishan piglets. Using a real-time PCR approach, expression profiles were determined for key TLR4 signaling pathway genes TLR4, MyD88, CD14, IFN-α, IL-1β and TNF-α in the spleen, thymus, lymph nodes, duodenum and jejunum of E. coli F18-resistant and -sensitive animals. TLR4 signaling pathway genes were expressed in all the immune organs and intestinal tissues, and the expression was generally higher in the spleen and lymph nodes. TLR4 transcription was higher in the spleen of sensitive piglets (p<0.05), but there was no significant difference in TLR4 mRNA levels in other tissues. Similarly, CD14 transcription was higher in lymph nodes of sensitive animals (p<0.05) but not in other tissues. IL-1β expression was higher in the spleen and in the duodenum of resistant piglets (p<0.05, p<0.01, respectively), and there were no significant differences in other tissues. There were also no significant differences in the expression of MyD88, TNF-α and IFN-α between sensitive and resistant piglets (p>0.05). These results further confirm the involvement of the TLR4 signaling pathway in resistance to E. coli F18 in Meishan weaned piglets. The resistance appeared to be mediated via downregulation of TLR4 and CD14, and upregulation of MyD88 that may promote the release of cytokines TNF-α, IL-1β, IFN-α and other inflammatory mediators which help to fight against E. coli F18 infection.
    Źródło:
    Polish Journal of Veterinary Sciences; 2016, 19, 2
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Use of fluorescence quantitative polymerase chain reaction (PCR) for the detection of Escherichia coli adhesion to pig intestinal epithelial cells
    Autorzy:
    Dai, C.H.
    Gan, L.N.
    Qin, W.U.
    Zi, C.
    Zhu, G.Q.
    Wu, S.L.
    Bao, W.B.
    Powiązania:
    https://bibliotekanauki.pl/articles/31510.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    An efficient and accurate method to test Escherichia coli (E. coli) adhesion to intestinal epithelial cells will contribute to the study of bacterial pathogenesis and the function of genes that encode receptors related to adhesion. This study used the quantitative real-time polymerase chain reaction (qPCR) method. qPCR primers were designed from the PILIN gene of E. coli F18ab, F18ac, and K88ac, and the pig β-ACTIN gene. Total deoxyribonucleic acid (DNA) from E. coli and intestinal epithelial cells (IPEC-J2 cells) were used as templates for qPCR. The 2−ΔΔCt formula was used to calculate the relative number of bacteria in cultures of different areas. We found that the relative numbers of F18ab, F18ac, and K88ac that adhered to IPEC-J2 cells did not differ significantly in 6-, 12-, and 24-well culture plates. This finding indicated that there was no relationship between the relative adhesion number of E. coli and the area of cells, so the method of qPCR could accurately test the relative number of E. coli. This study provided a convenient and reliable testing method for experiments involving E. coli adhesion, and also provided innovative ideas for similar detection methods.
    Źródło:
    Polish Journal of Veterinary Sciences; 2016, 19, 3
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Protozoa in a stressed area of the Egyptian Mediterranean coast of Damietta, Egypt
    Autorzy:
    Dorgham, M.M.
    El-Tohamy, W.S.
    Abdel-Aziz, N.E.
    El-Ghobashi, A.
    Qin, J.G.
    Powiązania:
    https://bibliotekanauki.pl/articles/49061.pdf
    Data publikacji:
    2013
    Wydawca:
    Polska Akademia Nauk. Instytut Oceanologii PAN
    Tematy:
    environment condition
    pollution indicator
    tintinnid
    Protozoa
    Egyptian Mediterranean coast
    Egypt
    Diametta coast
    aquatic ecosystem
    coastal water
    eutrophication
    Źródło:
    Oceanologia; 2013, 55, 3
    0078-3234
    Pojawia się w:
    Oceanologia
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    In vitro production of Sudanese camel (Camelus dromedarius) embryos from epididymal spermatozoa and follicular oocytes of slaughtered animals
    Autorzy:
    Abdelkhalek, A.E.
    Gabr, S.A.
    Khalil, W.A.
    Shamiah, S.H.
    Pan, L.
    Qin, G.
    Farouk, M.H.
    Powiązania:
    https://bibliotekanauki.pl/articles/31345.pdf
    Data publikacji:
    2017
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    Application of assisted reproductive technology in camelidea, such as artificial insemination (AI) and embryo transfer, has been slow in comparison to that for other livestock species. In Egypt, there are few attempts to establish in vitro maturation (IVM) and fertilization (IVF) techniques in dromedary camel. The present study was carried out to produce Sudanese camel embryos using in vitro matured oocytes and epididymal spermatozoa. Dromedary camel ovaries were collected from abattoirs and then, the oocytes were aspirated from all the visible follicles on the ovarian surface (~2-8 mm in a diameter). Meanwhile, Fetal Dromedary Camel Serum (FDCS) was obtained from camel fetuses after slaughtering. Thereafter, only Cumulus Oocyte Complexes (COCs) were matured in vitro in the Tissue Culture Medium (TCM-199) complemented with 10% FDCS. Spermatozoa required for in vitro fertilization were collected from testes (epididymal cauda) of the slaughtered camel bulls. The results clearly showed that the maturation rate of oocytes at metaphase II was about 59.5% while the fertilization rate was around 70.4%. Intriguingly, the embryo rates determined were 13.1%, in 2-cell; 0.0%, in 4-cell; 34.7%, in 8-16% cell; 39.1%, in morula and 13.1% in a blastocyst stage. This study represented a successful in vitro production of Sudanese dromedary camel embryos from epididymal sperm cells and in vitro matured oocytes recovered from slaughtered camels.
    Źródło:
    Polish Journal of Veterinary Sciences; 2017, 20, 1
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-6 z 6

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