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Wyszukujesz frazę "methicillin" wg kryterium: Wszystkie pola


Tytuł:
Antibiotic susceptibility of methicillin - resistant and methicillin - suspectible coagulase-negative staphylococci isolated from bovine mastitis
Autorzy:
Bochniarz, M.
Wawron, W.
Powiązania:
https://bibliotekanauki.pl/articles/32123.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The aim of the present study was to evaluate the antibiotic susceptibility of methicillin-susceptible (MS) and methicillin-resistant (MR) coagulase-negative Staphylococcus (CNS) strains isolated from milk of cows with mastitis. The study was conducted on 100 CNS strains (20 MRCNS and 80 MSCNS) isolated from milk samples of 86 cows from the Lublin (Poland) region farms. Antibiotic susceptibility of microorganisms was evaluated using the disc-diffusion method on the Mueller-Hinton agar according to the guidelines of the National Committee for Clinical Laboratory Standards (NCCLS). The highest efficacy against MSCNS was demonstrated for cephalosporin antibiotics, i.e. cefacetril (91.3%), ceftiofur (67.5%), cefoperazone (66.3%) and cephalexin (60.0% of susceptible MSCNS strains). Moreover, a high percentage of vancomycin-susceptible strains was demonstrated (83. 8%). The activity of combination of amoxicillin with clavulanic acid and gentamicin was found weaker (63.8% and 61.3% of susceptible strains, respectively). About 50.0% of MSCNS were susceptible to erythromycin, enrofloxacine and amoxicillin. A large proportion of CNS was resistant to neomycin, penicillin, tetracycline, streptomycin, lincomycin and ampicillin (28.8%, 30.0%, 31.3%, 31.3%, 33.8% and 33.8% of susceptible strains, respectively). The highest percentage of MRCNS was susceptible to vancomycin (75.0%), erythromycin (65.0%) and streptomycin (50.0%). Their susceptibility to enrofloxacine (35.0%) as well as gentamicin and tetracycline (30.0%) was markedly lower. The lowest activity was found for lincomycin and neomycin (20.0% of susceptible MRCNS strains, each).
Źródło:
Polish Journal of Veterinary Sciences; 2011, 14, 3
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Lack of correlation between X region spa polymorphism and virulence of methicillin resistant and methicillin sensitive Staphylococcus aureus strains
Autorzy:
Kurlenda, Julianna
Grinholc, Mariusz
Szweda, Piotr
Powiązania:
https://bibliotekanauki.pl/articles/1040442.pdf
Data publikacji:
2010
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
polymorphism
protein A
spa
virulence
Opis:
Staphylococcus aureus is an etiological factor of severe infections in both hospital and ambulatory environments. As methicillin resistant Staphylococcus aureus strains spread quickly across healthcare centers resulting in life-threatening infections with increased mortality, they are considered more virulent than MSSA strains. Protein A, encoded by the spa gene, is one of the virulence factors involved in the staphylococcal pathogenesis. It has been suggested that the number of 24-bp tandem repeat units along the X region of the spa gene correlates with the virulence level of the strains. The current work analyzed the relationships between the virulence of MRSA and MSSA strains with region X polymorphism. No obvious correlation was observed.
Źródło:
Acta Biochimica Polonica; 2010, 57, 1; 135-138
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Drug resistance profile and detection of genes responsible for methicillin resistance in Staphylococcus aureus isolated from municipal waste
Autorzy:
Wolny-Koładka, Katarzyna
Powiązania:
https://bibliotekanauki.pl/articles/2203548.pdf
Data publikacji:
2023
Wydawca:
Instytut Technologiczno-Przyrodniczy
Tematy:
drug resistance
mecA gene
methicillin resistance
municipal waste
Staphylococcus aureus
Opis:
Currently, we are facing the ever-increasing phenomenon of bacteria being resistant to antibiotics. It is the consequence of excessive and incorrect use of drugs. The phenomenon is a global problem affecting bacteria both in their hospital population and in the natural environment. Municipal waste is an environment conducive to the development of microorganisms, therefore it contains various groups of bacteria, including drug-resistant staphylococci. The aim of the study was to identify species of bacteria, determine their antibiotic resistance, and assess the occurrence of genes responsible for methicillin resistance in Staphylococcus aureus isolated from mixed municipal waste. Strains were isolated by Koch’s serial dilution method with the use of microbiological media. Species were identified using the MALDI TOF-MS technique, whereas the drug resistance profile was determined by disk diffusion and molecular PCR methods. 250 isolates of S. aureus were collected. The highest resistance found was to cefoxitin, erythromycin and tetracycline. Among the bacteria collected, resistance to 1, 2, 3 or 4 antibiotics at the same time was the most common, with a maximum of 10. Additionally, 45 (18%) MDR (multidrug-resistant) isolates were detected. Methicillin resistance was found by the disk diffusion test in 60 (24%) strains, while the mecA gene was detected in as many as 180 (72%) isolates.
Źródło:
Journal of Water and Land Development; 2023, 56; 136--141
1429-7426
2083-4535
Pojawia się w:
Journal of Water and Land Development
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Heterogeneity of methicillin-sensitive Staphylococcus pseudintermedius strains isolated from diseased dogs
Autorzy:
Garbacz, K.
Piechowicz, L.
Zarnowska, S.
Haras, K.
Dabrowska-Szponar, M.
Powiązania:
https://bibliotekanauki.pl/articles/32033.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
animal disease
dog
heterogeneity
methicillin
Staphylococcus pseudintermedius
isolation
diseased animal
pulsed-field gel electrophoresis
Opis:
Thirty nine canine S. pseudintermedius strains were examined for antibiotic susceptibility and genetic polymorphisms. All strains were methicillin-sensitive S. pseudintermedius (MSSP). Resistance to penicillin was most prevalent (66.6%), followed by resistance to neomycin (56.4%), erythromycin (53.8%), clindamycin (48.7%), chloramphenicol (48.7%), and tetracycline (46.2%). Pulsed-field electrophoresis (PFGE) showed a high genetic polymorphism in the investigated strains.
Źródło:
Polish Journal of Veterinary Sciences; 2011, 14, 2
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Evidence of new sequence types of methicillin-resistant Staphylococcus pseudintermedius in Italy
Autorzy:
Nocera, F.P.
Parisi, A.
Corrente, M.
De Martino, L.
Powiązania:
https://bibliotekanauki.pl/articles/2087290.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
methicillin-resistant S. pseudintermedius
canine skin diseases
Multi-Locus Sequence Typing
new sequence types
Źródło:
Polish Journal of Veterinary Sciences; 2020, 23, 3; 465-468
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Self-reported methicillin-resistant Staphylococcus aureus infection in USA pork producers
Autorzy:
Leedom, Larson K R
Smith, T.C.
Donham, K.J.
Powiązania:
https://bibliotekanauki.pl/articles/51288.pdf
Data publikacji:
2010
Wydawca:
Instytut Medycyny Wsi
Tematy:
pork production
pork producer
USA
animal farm
pig
agricultural worker
human disease
infection
methicillin-resistant Staphylococcus aureus
Staphylococcus aureus
Źródło:
Annals of Agricultural and Environmental Medicine; 2010, 17, 2; 331-334
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Resistance to methicillin of coagulase-negative staphylococci (CNS) isolated from bovine mastitis
Autorzy:
Bochniarz, M
Wawron, W
Szczubial, M.
Powiązania:
https://bibliotekanauki.pl/articles/31477.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The aim of this study was to determine the mechanisms of staphylococcal resistance to methicillin. CNS (n=100 isolates) were prepared from the mammary inflammatory secretions of 86 cows from farms located in the Lublin region. Methicillin-resistant isolates constituted 20.0% of all CNS. Staphylococcus sciuri (n=8) and Staphylococcus xylosus (n=6) were most abundant, followed by Staphylococcus chromogenes (n=3), Staphylococcus haemolyticus (n=2) and Staphylococcus warneri (n=1). The mecA gene was found in 50.0% of MRCNS (10.0% of all CNS isolates) belonging to two species: S. sciuri and S. xylosus. All mecA-positive isolates contained the protein of low affinity to penicillin (penicillin-binding protein 2a – PBP2a). The enzyme hydrolysing the β-lactam ring in antibiotics was detected in 40.0% of MRCNS; 10.0% of MRCNS isolates were characterised by the presence of the mecA gene and ability to produce β-lactamase. The remaining 20.0% of MRCNS isolates showing phenotypic resistance to methicillin were mecA gene-negative and were not able to produce β-lactamase.
Źródło:
Polish Journal of Veterinary Sciences; 2013, 16, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The inhibition of methicillin-resistant Staphylococcus aureus by essential oils isolated from leaves and fruits of Schinus areira depending on their chemical compositions
Autorzy:
Celaya, Liliana
Alabrudzińska, Marta
Molina, Ana
Viturro, Carmen
Moreno, Silvia
Powiązania:
https://bibliotekanauki.pl/articles/1039327.pdf
Data publikacji:
2014
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Schinus areira
essential oils
antibacterial
Methicillin-resistant Staphylococcus aureus
Opis:
Schinus areira L. is a native plant from South America used for centuries in traditional medicine. Here, we investigate the antimicrobial activity of four essential oils extracted from leaves and fruits of S. areira exhibiting different chemical profiles. The antibacterial activity against the human pathogenic bacteria Staphylococcus aureus susceptible as well as methicillin resistant strain was assessed by the broth microdilution assay. The results showed that the limonene-rich oil extracted from the leaves and fruits have potent antibacterial effect on S. aureus ATCC 25923, while the α-phellandrene-rich fruit oil having a lower content of limonene showed the lowest antibacterial efficacy. In this work, for the first time, we demonstrated the bactericidal activity of essential oils isolated from fruits and leaves of S. areira against susceptible and methicillin resistant S. aureus strains. All results point out the potential use of the S. areira oils as antimicrobial agents to be used, at least against Staphylococcal infections.
Źródło:
Acta Biochimica Polonica; 2014, 61, 1; 41-46
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Molecular characterization and detection of enterotoxins, methicillin resistance genes and antimicrobial resistance of Staphylococcus aureus from fish and ground beef
Autorzy:
Arslan, S.
Ozdemir, F.
Powiązania:
https://bibliotekanauki.pl/articles/30390.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
A total of 120 samples including 40 freshwaterfish(Oncorhynchus mykiss), 40 seawater fish (Sparus aurata) and 40 ground beef samples were examined for the presence of Staphylococcus aureus. The isolates were identified using biochemical tests and a PCR for the species-specific fragment (Sa442) and thermonuclease gene (nucA). The presence of staphylococcal enterotoxin genes (sea, seb, sec, sed and see), toxin genes (eta, etb, tsst), methicillin resistance gene (mecA) and some phenotypic virulence factors was also tested. Genotypic characterization of the isolates was analyzed by PCR-RFLP of the coa gene. Overall, 36 (30%) meat samples were contaminated with S. aureus. Of the 36 isolates, 3 (8.3%) were found to be positive for enterotoxin genes. Only 1 isolate (5.9%) from ground beef had the sea gene. In addition, 1 (12.5%) of the freshwater fish and 1 (9.1%) of the seawater fish carried both the sea and sed genes. The presence of seb, sec, see, eta, etb and tsst was not detected among the isolates of S. aureus. The amplified coa gene revealed five different clusters. Seven and six distinct RFLP patterns were obtained with AluI and HaeIII digestion, respectively. All isolates were found to be positive for slime, hemolytic and DNase activity while 41.7% of them were beta-lactamase positive. The presence of methicillin resistance was neither detected by PCR nor the disk diffusion method. A total of 94.4% of the isolates were resistant to at least one antimicrobial while 44.4% of them were resistant to at least two or more antimicrobials.
Źródło:
Polish Journal of Veterinary Sciences; 2017, 20, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Production of bioactive metabolites from different marine endophytic Streptomyces species and testing them against methicillin-resistant Staphylococcus aureus (MRSA) and cancer cell lines
Autorzy:
El-Gendy, M.M.A.A.
Mohamed, Z.K.
Hekal, N.Z.
Ali, F.M.
Yousef, A.E.M.
Powiązania:
https://bibliotekanauki.pl/articles/81078.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Staphylococcus aureus
infection
methicillin-resistant Staphylococcus aureus
endophyte
Streptomyces
bioactive metabolite
Actinomycetes
marine invertebrate
anticancer activity
cancer cell line
lung cancer
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2018, 99, 1
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
First report of swine-associated methicillin-resistant Staphylococcus aureus ST398 in Lithuania
Autorzy:
Ruzauskas, M.
Couto, N.
Belas, A.
Klimiene, I.
Siugzdiniene, R.
Pomba, C.
Powiązania:
https://bibliotekanauki.pl/articles/30638.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
During 2011, 160 nasal samples were taken from pigs on 8 different farms in Lithuania. Four methicillin-resistant Staphylococcus aureus (MRSA) isolates were obtained. The isolates were ST398, spa type t011 and SCCmec V and none carried the lukF/lukS genes. Strains were resistant to tetracycline, attributed to tetK and tetM genes, and to erythromycin owing to the ermB gene. One MRSA strain was resistant to trimethoprim/sulfamethoxazole and carried the dfrK gene. This is the first report on the presence and characteristics of livestock-associated MRSA isolated from pigs in Lithuania.
Źródło:
Polish Journal of Veterinary Sciences; 2013, 16, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Occupational acquisition of methicillin-resistant Staphylococcus aureus in humans – A description of MRSA carrier and infected cases from the Region of North Jutland in Denmark
Autorzy:
Omland, O.
Hoffmann, L.
Powiązania:
https://bibliotekanauki.pl/articles/51755.pdf
Data publikacji:
2012
Wydawca:
Instytut Medycyny Wsi
Źródło:
Annals of Agricultural and Environmental Medicine; 2012, 19, 4
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Staphylococcus aureus as an infectious agent: overview of biochemistry and molecular genetics of its pathogenicity
Autorzy:
Plata, Konrad
Rosato, Adriana
Węgrzyn, Grzegorz
Powiązania:
https://bibliotekanauki.pl/articles/1040468.pdf
Data publikacji:
2009
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Staphylococcus aureus
virulence
pathogenicity genes
toxins
methicillin resistance
Opis:
Although it is estimated that 20-30% of the general human population are carriers of Staphylococcus aureus, this bacterium is one of the most important etiological agents responsible for healthcare-associated infections. The appearance of methicillin resistant S. aureus (MRSA) strains has created serious therapeutical problems. Detailed understanding of the mechanisms of S. aureus infections seems necessary to develop new effective therapies against this pathogen. In this article, we present an overview of the biochemical and genetic mechanisms of pathogenicity of S. aureus strains. Virulence factors, organization of the genome and regulation of expression of genes involved in virulence, and mechanisms leading to methicilin resistance are presented and briefly discussed.
Źródło:
Acta Biochimica Polonica; 2009, 56, 4; 597-612
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Bactericidal effect of photodynamic therapy against methicillin-resistant Staphylococcus aureus strain with the use of various porphyrin photosensitizers
Autorzy:
Grinholc, Mariusz
Szramka, Bozena
Olender, Katarzyna
Graczyk, Alfreda
Powiązania:
https://bibliotekanauki.pl/articles/1041060.pdf
Data publikacji:
2007
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
photosensitizer
porphyrin
photodynamic therapy
MRSA
Opis:
Photodynamic therapy (PDT) is based on photosensitizers activated by light of appropriate wavelength. Their activation leads to generation of singlet oxygen and free radicals responsible for the cytotoxic effect. The aim of this project was to compare the bactericidal effect of PDT using different porphyrin photosensitizers against a methicillin-resistant Staphylococcus aureus strain. Exogenous sensitizers (protoporphyrin IX and newly synthesized derivative, protoporphyrin diarginate) induced a 3 log10-unit reduction in bacterial viable counts. With the use of endogenous, ALA-induced porphyrins, a 1.6 log10-unit reduction was obtained. The sensitizers tested executed their antibacterial activity with no essential change in the antibiotic resistance pattern of the studied strain.
Źródło:
Acta Biochimica Polonica; 2007, 54, 3; 665-670
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Photodynamic effect of protoporphyrin diarginate (PPArg2) on methicillin-resistant Staphylococcus aureus and human dermal fibroblasts
Autorzy:
Grinholc, Mariusz
Kawiak, Anna
Kurlenda, Julianna
Graczyk, Alfreda
Bielawski, Krzysztof
Powiązania:
https://bibliotekanauki.pl/articles/1040819.pdf
Data publikacji:
2008
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
cytotoxicity
antimicrobial photodynamic inactivation
MRSA
fibroblasts
phototoxicity
Opis:
The worldwide rise in the antibiotic resistance of bacteria forces the development of alternative antimicrobial treatments. A potential approach is photodynamic inactivation (PDI). The aim of the present study was to determine the phototoxicity of protoporphyrin diarginate (PPArg2) against methicillin-resistant Staphylococcus aureus and human dermal fibroblasts. Different concentrations (0 to 20 µM) of PPArg2 and light dose of 6 J cm-2 were tested. Cell viability was evaluated using the methylthiazoletetrazolium (MTT) assay. Incubation with 10 µM followed by illumination yielded a 3.6 log10-unit reduction in the viable count for Staphylococcus aureus. At the same experimental conditions, only 22.5% of the fibroblasts were photoinactivated. Protoporphyrin diarginate at concentrations up to 20 µM demonstrated no toxicity towards S. aureus or fibroblasts when not irradiated. These results suggest that the protoporphyrin diarginate exerts a high bactericidal effect against methicillin-resistant S. aureus strain without harming eukaryotic cells.
Źródło:
Acta Biochimica Polonica; 2008, 55, 1; 85-90
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł

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