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Wyświetlanie 31-45 z 116
Tytuł:
Antimicrobial activity of polyphenols extracted from Thai medical plants on extended-spectrum beta-lactamase-producing Escherichia coli isolates from healthy dairy cows
Autorzy:
Konputtar, A.
Aengwanich, W.
Saraphol, B.
Yossapol, M.
Powiązania:
https://bibliotekanauki.pl/articles/16614628.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
antimicrobial resistance
Escherichia coli ATCC 25922
Psidium guajava Linn
Thailand
Opis:
Escherichia coli producing extended-spectrum beta-lactamase (ESBL) are antimicrobial-resistant Enterobacteriaceae important in the livestock production sector, especially dairy cows because these antimicrobial-resistant bacteria can be transferred to consumers via consumption. If antimicrobials are continually used in dairy farms, this may result in antimicrobial resistance. Therefore, investigation of antimicrobial resistance and finding new alternative methods for inhibiting ESBL-producing E. coli is essential. Hence, the aim of this study was to examine the ability of selected antimicrobials to inhibit E. coli ATCC 25922, control bacteria and ESBL-producing E. coli isolated from dairy farms. We also investigated the capacity of polyphenol extract from 10 varieties of medicinal plants to inhibit ESBL-producing E. coli using a broth microdilution method. It was found that control bacteria were susceptible to all antimicrobial agents, i.e., ampicillin, cefotaxime, ciprofloxacin, chloramphenicol, gentamycin, imipenem, nalidixic acid, tetracycline, and sulfamethoxazole/ trimethoprim. However, ESBL-producing E. coli exhibited both susceptibility and resistance to selected antimicrobials. The polyphenol extracted from Psidium guajava Linn at the lowest concentration was 4.5 mg/mL, which could inhibit control bacteria, but at the same concentration could not inhibit ESBL-producing E. coli. These phenomena indicated that ESBL-producing E. coli had both susceptibility and resistance to antimicrobials. Polyphenol, which could inhibit non-resistant E. coli, could not inhibit ESBL-producing E. coli.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 4; 501-510
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Extra-intestinal pathogenic Escherichia coli – threat connected with food-borne infections
Autorzy:
Wasiński, B.
Powiązania:
https://bibliotekanauki.pl/articles/2085200.pdf
Data publikacji:
2019
Wydawca:
Instytut Medycyny Wsi
Tematy:
food
food-borne pathogens
extra-intestinal pathogenic escherichia coli
ExPEC
Opis:
Infections caused by extra-intestinal pathogenic Escherichia coli (ExPEC) are a serious public health problem worldwide. The most troublesome are urinary tract infections, severe neonatal meningitis, serious intraabdominal infections, and more rarely, pneumonia, intravascular-device infections, osteomyelitis, soft-tissue infections or sometimes bacteraemia. These strains are also able cause significant economic losses in animal husbandry. A thorough understanding of ExPEC ecology, reservoirs, chains and dynamics of transmission can greatly contribute to a reduction in the burden of ExPEC-associated disease. The ability of E. coli (including ExPEC) to exist and survive in various ecological niches impedes the precise recognition and indication of transmission routes most important for individual infections cases. Among many identified ExPEC reservoirs, animal companion and animals providing food seem to be important sources of infection for human; however, the real level of risk connected with potential transmission of these bacteria remains unclear. Food is indicated as one of potential ways of transmission. Despite a quite high number of reports, many of the uncertainties are expected to be reliably elucidated. This review presents most important data on the current state of knowledge concerning the potential role of food in ExPEC transmission. The possible consequences of ExPEC infections in human and animals are briefly described.
Źródło:
Annals of Agricultural and Environmental Medicine; 2019, 26, 4; 532-537
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Reversion of argE3 ochre strain Escherichia coli AB1157 as a tool for studying the stationary-phase (adaptive) mutations.
Autorzy:
Nowosielska, Anetta
Grzesiuk, Elżbieta
Powiązania:
https://bibliotekanauki.pl/articles/1044376.pdf
Data publikacji:
2000
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
mutY
replica plating
Arg+ revertants
Escherichia coli
adaptive mutations
mutT
Opis:
Adaptive (starvation-associated) mutations occur in non-dividing cells and allow growth under the selective conditions imposed. We developed a new method for the determination of adaptive mutations in Escherichia coli. The system involves reversion to prototrophy of the argE3OC mutation and was tested on AB1157 strains mutated in the mutT and/or mutY genes. The bacteria that mutated adaptively grow into colonies on minimal medium plates devoid of arginine (starvation conditions) when incubated longer than 4 days. Using the replica plating method we solved the problem of discrimination between growth-dependent and adaptive argE3→Arg+ revertants. Phenotype analysis and susceptibility of the Arg+ revertants to a set of T4 phage mutants create an additional possibility to draw a distinction between these two types of Arg+ revertants.
Źródło:
Acta Biochimica Polonica; 2000, 47, 2; 459-467
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Biotechnological conversion of glycerol from biofuels to 1,3-propanediol using Escherichia coli
Autorzy:
Przystałowska, Hanna
Lipiński, Daniel
Słomski, Ryszard
Powiązania:
https://bibliotekanauki.pl/articles/1039124.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
bioconversion
biodiesel
carbon source
Escherichia coli
glycerol
1,3-propanediol
Opis:
In the face of shortage of fossil fuel supplies and climate warming triggered by excessive carbon dioxide emission, alternative resources for chemical industry have gained considerable attention. Renewable resources and their derivatives are of particular interest. Glycerol, which constitutes one of the by-products during biodiesel production, is such a substrate. Thus, generated excess glycerol may become an environmental problem, since it cannot be disposed of in the environment. The most promising products obtained from glycerol are polyols, including 1,3-propanediol, an important substrate in the production of synthetic materials, e.g. polyurethanes, unsaturated polyesters, and epoxy resins. Glycerol can be used as a carbon and energy source for microbial growth in industrial microbiology to produce 1,3-propanediol. This paper is a review of metabolic pathways of native producers and E. coli with the acquired ability to produce the diol via genetic manipulations. Culture conditions during 1,3-PDO production and genetic modifications of E. coli used in order to increase efficiency of glycerol bioconversion are also described in this paper.
Źródło:
Acta Biochimica Polonica; 2015, 62, 1; 23-34
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Thermostable Pyrococcus woesei β-D-galactosidase - high level expression, purification and biochemical properties
Autorzy:
Wanarska, Marta
Kur, Józef
Pladzyk, Radosław
Turkiewicz, Marianna
Powiązania:
https://bibliotekanauki.pl/articles/1041317.pdf
Data publikacji:
2005
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Pyrococcus woesei
β-d-galactosidase
Escherichia coli
purification
expression system
Opis:
The gene encoding β-D-galactosidase from Pyrococcus woesei was PCR amplified, cloned, expressed in Escherichia coli under the control of an inducible T7 promoter, purified and characterized. The expression system was developed by the construction of recombinant plasmid, based on the high copy number pUET1 vector, giving four times more efficient expression of P. woesei β-D-galactosidase (20 mg of enzyme from 1 liter of culture) than that obtained from a previously constructed one. The recombinant enzymes were purified in a two-step procedure: double heat-denaturation of E. coli cell proteins and affinity chromatography on p-aminobenzyl 1-thio-β-D-galactopyranoside-agarose. To achieve efficient purification of P. woesei β-D-galactosidase by immobilized metal-ion affinity chromatography (IMAC), a His-tag was placed either at the N- or the C-terminal of the coding sequence. The obtained fusion proteins revealed the same specific activity of approximately 5400 U/mg, which was 10 times lower than the wild-type β-D-galactosidase (51100 U/mg). The activity of P. woesei β-D-galactosidase was enhanced by thiol compounds, Mg2+ ions and D-galactose, and was inhibited by heavy metal ions and D-glucose, while Ca2+ ions had no effect.
Źródło:
Acta Biochimica Polonica; 2005, 52, 4; 781-787
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Częstość występowania w wodzie nietypowych w stosunku do laktozy bakterii grupy Coli
The frequency of occurance of non-typical lactose-fermenting coliforms (Lnt) in water
Autorzy:
Zieminska, S.
Haman, S.
Hrynko, N.
Mackiewicz, K.
Plata, D.
Sinkiewicz, J.
Smolarek, E.
Wasilewska, E.
Powiązania:
https://bibliotekanauki.pl/articles/877283.pdf
Data publikacji:
1977
Wydawca:
Narodowy Instytut Zdrowia Publicznego. Państwowy Zakład Higieny
Tematy:
Escherichia coli
laktoza
woda
proba wodna
woda pitna
wymagania sanitarne
Źródło:
Roczniki Państwowego Zakładu Higieny; 1977, 28, 6
0035-7715
Pojawia się w:
Roczniki Państwowego Zakładu Higieny
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Zastosowanie immunofluorescencji w mikrobiologii wody na przykładzie wybranych chorobotwórczych szczepów Escherichia coli. Cz.II. Badania z zakażaną wodą naturalną
The use of immunofluorescence in water microbiology as exemplified by selected strains of enteropathogenic Escherichia coli Part.II. Studies on infected natural water
Autorzy:
Danielsson, D.
Zieminska, S.
Powiązania:
https://bibliotekanauki.pl/articles/871783.pdf
Data publikacji:
1973
Wydawca:
Narodowy Instytut Zdrowia Publicznego. Państwowy Zakład Higieny
Tematy:
mikrobiologia wod
immunofluorescencja
szczepy chorobotworcze
Escherichia coli
woda
badania naukowe
zanieczyszczenia wod
Źródło:
Roczniki Państwowego Zakładu Higieny; 1973, 24, 3
0035-7715
Pojawia się w:
Roczniki Państwowego Zakładu Higieny
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Virulence and antibiotic resistance of Escherichia coli isolated from rooks
Autorzy:
Kmet, V.
Drugdova, Z.
Kmetova, M.
Stanko, M.
Powiązania:
https://bibliotekanauki.pl/articles/51141.pdf
Data publikacji:
2013
Wydawca:
Instytut Medycyny Wsi
Tematy:
virulence
antibiotic resistance
Escherichia coli
isolation
rook
polymerase chain reaction
DNA microarray
Opis:
With regard to antibiotic resistance studies in various model animals in the urban environment, the presented study focused on the rook, many behavioural and ecological aspects of which are important from an epidemiological point of view. A total of 130 Escherichia coli strains isolated from rook faeces during a two-year period (2011–2012) were investigated for antibiotic resistance and virulence. Resistance to ampicillin (60%) and streptomycin (40%) were the most frequent, followed by resistance to fluoroquinolones (ciprofloxacin-22% and enrofloxacin-24%), tetracycline (18%), cotrimoxazol (17%) and florfenicol (14%). Ceftiofur resistance occured in 10.7 % of strains and cefquinom resistance in 1.5 % of strains. Twenty-five E.coli strains with a higher level of MICs of cephalosporins (over 2mg/L of ceftazidime and ceftriaxon) and fluoroquinolones were selected for detection of betalactamase genes (CTX-M, CMY), plasmid-mediated quinolone resistance qnrS, integrase 1, and for APEC (avian pathogenic E.coli) virulence factors (iutA, cvaC, iss, tsh, ibeA, papC, kpsII). Genes of CTX-M1, CMY-2, integrase 1, papC, cvaC, iutA were detected in one strain of E.coli, and qnrS, integrase 1, iss, cvaC, tsh were detected in another E.coli. DNA microarray revealed the absence of verotoxin and enterotoxin genes and pathogenicity islands. The results show that rooks can serve as a reservoir of antibiotic-resistant E. coli with avian pathogenic virulence factors for the human population, and potentially transmit such E.coli over long distances.
Źródło:
Annals of Agricultural and Environmental Medicine; 2013, 20, 2
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Development and application of isothermal amplification methods for rapid detection of F4 fimbriae producing Escherichia coli
Autorzy:
Zhao, L.Y.
Niu, J.H.
Gao, X.L.
Liu, C.N.
Liu, S.M.
Jiang, N.
Lv, X.P.
Zheng, S.M.
Powiązania:
https://bibliotekanauki.pl/articles/2087340.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Escherichia coli
F4 fimbriae
isothermal amplification
rapid detection
clinical samples
Źródło:
Polish Journal of Veterinary Sciences; 2020, 23, 1; 143-152
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Single-stranded DNA-binding proteins (SSBs) - sources and applications in molecular biology.
Autorzy:
Kur, Józef
Olszewski, Marcin
Filipkowski, Paweł
Powiązania:
https://bibliotekanauki.pl/articles/1041357.pdf
Data publikacji:
2005
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Thermus aquaticus
Thermus thermophilus
Escherichia coli
PCR
recombination
DNA replication
SSB
Opis:
Single-stranded DNA-binding proteins (SSBs) play essential roles in DNA replication, recombination, and repair in bacteria, archaea and eukarya. The SSBs share a common core ssDNA-binding domain with a conserved OB (oligonucleotide/oligosaccharide binding) fold. This ssDNA-binding domain was presumably present in the common ancestor to all three major branches of life. In recent years, there has been an increasing interest in SSBs because they are useful for molecular biology methods and for analytical purposes. In this review, we concentrate on recent advances in the discovery of new sources of SSBs as well as certain aspects of their applications in analytical sciences.
Źródło:
Acta Biochimica Polonica; 2005, 52, 3; 569-574
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Studies on biosynthesis of silver nanoparticles using Rhizopus sp. and its antibacterial efficacy on E. coli MDR strains
Autorzy:
Hiremath, J.
Rathod, V.
Ninganagouda, S.
Singh, D.
Prema, K.
Powiązania:
https://bibliotekanauki.pl/articles/11479.pdf
Data publikacji:
2014
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
biosynthesis
silver nanoparticle
Rhizopus
antibacterial activity
Escherichia coli
multidrug resistance
nanotechnology
Opis:
Nanotechnology is a field that is burgeoning day by day, making an impact in all spheres of human life. Biological methods of synthesis have paved way for the “greener synthesis” of nanoparticles and these have proven to be better methods due to slower kinetics, they offer better manipulation and control over crystal growth and their stabilization. In this context we have investigated extracellular biosynthesis of silver nanoparticles (AgNPs) using cell-free extract of Rhizopus spp.. Formation of AgNPs was indicated by the change in the colour of the cellfree extract from yellow to dark brown under static condition after 48 hrs of incubation. Characterization of AgNPs was carried out by UV-Vis Spectroscopy which gave sharp plasmon resonance peak at 429 nm corresponding to spherical shaped nanoparticles. Transmission electron microscopy (TEM) micrograph showed formation of well-dispersed AgNPs in the range of 25-50 nm. Scanning electron microscopy (SEM) showed the particles to be uniformly dispersed without agglomeration with smooth morphology. EDS showed the presence of elemental silver at 3kev. X-ray diffraction (XRD)-spectrum of the AgNPs exhibited 2θ¸ values corresponding to nanocrystal. These biosynthesized AgNPs were used to study their antimicrobial activity against Multi-drug resistant (MDR) E. coli strains, by Agar diffusion method. Zone of inhibition was measured. Synthesis of nanosized particles with antibacterial properties, which are called "nanoantibiotics", is of great interest in the development of new pharmaceutical products.
Źródło:
International Letters of Natural Sciences; 2014, 18
2300-9675
Pojawia się w:
International Letters of Natural Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Zastosowanie immunofluorescencji w mikrobiologii wody na przykładzie wybranych chorobotwórczych szczepów Escherichia coli. Cz.I. Badania wstępne z czystymi szczepami
The use of immunofluorescence in water microbiology as exemplified by selected strains of enteropathogenic Escherichia coli Part I. Preliminary investigations with pure strains
Autorzy:
Zieminska, S.
Swiderska, H.
Powiązania:
https://bibliotekanauki.pl/articles/876415.pdf
Data publikacji:
1973
Wydawca:
Narodowy Instytut Zdrowia Publicznego. Państwowy Zakład Higieny
Tematy:
mikrobiologia wod
szczepy chorobotworcze
Escherichia coli
immunofluorescencja
wody
scieki
badania naukowe
Źródło:
Roczniki Państwowego Zakładu Higieny; 1973, 24, 3
0035-7715
Pojawia się w:
Roczniki Państwowego Zakładu Higieny
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Assessing Groundwater Quality for Drinking Purpose in Jordan: Application of Water Quality Index
Autorzy:
Ibrahim, Mohamad Najib
Powiązania:
https://bibliotekanauki.pl/articles/124859.pdf
Data publikacji:
2019
Wydawca:
Polskie Towarzystwo Inżynierii Ekologicznej
Tematy:
water quality index
groundwater quality
drinking water
Escherichia coli
hydrochemistry
Jordan
Opis:
Groundwater is a key source of drinking water in Jordan. This study was conducted to assess the suitability of groundwater in major groundwater basins in Jordan for drinking purposes. The groundwater quality data from sixteen sampling stations within one-year-monitoring period from March 2015 to February 2016 were used. Weighted arithmetic water quality index (WQI) with respect to the Jordanian standards for drinking water was used for quality assessment. Sixteen Physical, chemical and microbiological parameters were selected to calculate WQI. The result showed that all physical and chemical parameters were almost below the maximum allowable level based on the Jordanian standards for drinking. On the other hand, the microbiological parameter (i.e. E.coli count) was exceeded the maximum allowable limit in all the studied locations based on the Jordanian standards for drinking water. The computed WQI values range from 40 to 4295. Therefore, out of 16 studied locations, three locations are classified in the “Excellent water” class, nine locations as a “Good water” class, one as a “Poor water” class, two as a “very poor water” class, and one as a “water unsuitable for drinking purpose” class. Furthermore, Escherichia coli is considered the most effective parameter on the determination of WQI in this study. This result highlighted the importance of including the microbiological parameters in any drinking water assessment, since they reflect with other physical and chemical parameters the actual condition of water quality for different purposes.
Źródło:
Journal of Ecological Engineering; 2019, 20, 3; 101-111
2299-8993
Pojawia się w:
Journal of Ecological Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Ocena toksyczności nanocząstek srebra w stosunku do lekoopornych szczepów Escherichia coli izolowanych z odpadów komunalnych
Evaluation of silver nanoparticles toxicity to drug-resistant Escherichia coli strains isolated from municipal waste
Autorzy:
Wolny-Koładka, K.
Sikora, A.
Malina, D.
Powiązania:
https://bibliotekanauki.pl/articles/101027.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Stowarzyszenie Infrastruktura i Ekologia Terenów Wiejskich PAN
Tematy:
Escherichia coli
lekooporność
nanocząstki srebra
toksyczność
drug resistance
silver nanoparticles
toxicity
Opis:
Celem pracy była ocena bakteriobójczych właściwości nanocząstek srebra w stosunku do bakterii Escherichia coli. Ponadto, wykonano analizę wrażliwości bakterii E. coli na powszechnie stosowane antybiotyki. W przeprowadzonych badaniach wykorzystano 51 szczepów izolowanych z odpadów komunalnych. Zastosowano wzrastające stężenia nanosrebra: 5, 10, 15, 30, 60 oraz 125 ppm. Kontrolą pozytywną był antybiotyk - ampicylina, negatywną - woda destylowana. Analizę lekooporności wykonano przy pomocy metody dyfuzyjno-krążkowej. Wraz ze wzrostem stężenia nanocząstek srebra, wzrastały ich właściwości antybakteryjne. Najmniejszym stężeniem hamującym wzrost niektórych izolatów było 5 ppm, natomiast najskuteczniejsze było stężenie 125 ppm. Analiza lekooporności wykazała, że antybiotykiem hamującym wzrost największej liczby szczepów E. coli była amikacyna. Ponadto, wykryto szczepy wielolekooporne stanowiące 22%. Nanosrebro posiada duży potencjał bakteriobójczy i może okazać się pomocne w rozwiązaniu problemu lekooporności. Jednak, zasadne jest prowadzenie dalszych badań oceniających wpływ toksyczności nanocząstek na środowisko i organizmy wyższe.
The aim of the study was to evaluate the bactericidal properties of silver nanoparticles against bacteria Escherichia coli. In addition, a sensitivity analysis was made of E. coli to commonly used antibiotics. In the study were used 51 strains isolated from municipal waste. Applied growing concentrations of nanosilver were used: 5, 10, 15, 30, 60 and 125 ppm. The positive control was an antibiotic - ampicillin, negative control - a distilled water. Drug-resistance analysis was performed by disc-diffusion method. Along with the increase in the concentration of silver nanoparticles their antibacterial properties is also growing. The smallest concentration that inhibited growth of some isolates was 5 ppm, while the most effective concentration was 125 ppm. Analysis of drug resistance showed that the antibiotic inhibits growth of a large number of E. coli strains was amikacin. In addition, 22% of all isolates analyzed were drug-resistant. Nanosilver has a high bactericidal potential and may be helpful in solving the problem of drug resistance. However, it is reasonable to conduct further studies on the effects of toxicity of nanoparticles on the environment and higher organisms.
Źródło:
Infrastruktura i Ekologia Terenów Wiejskich; 2018, I/1; 7-23
1732-5587
Pojawia się w:
Infrastruktura i Ekologia Terenów Wiejskich
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 31-45 z 116

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