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Wyświetlanie 1-15 z 34
Tytuł:
Analysis of potyvirus terminal protein VPg-transgenic Arabidopsis thaliana plants
Autorzy:
Wojtal, Izabela
Piontek, Paulina
Grzela, Renata
Jarmołowski, Artur
Zagórski, Włodzimierz
Chroboczek, Jadwiga
Powiązania:
https://bibliotekanauki.pl/articles/1039885.pdf
Data publikacji:
2011
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
VPg protein
Potyvirus
pathogen-derived resistance
transgenic plant
Opis:
Virus-coded VPg protein of Potato virus Y (PVY) does not have homologs apart from other VPgs. Since VPg is indispensable for the potyvirus life cycle, it appeared a good candidate for eliciting pathogen-derived resistance to PVY. Following agroinfection used to obtain PVY VPg-transgenic Arabidopsis thaliana plants, only few transgenic seeds were recovered giving rise to six transgenic plants that contained the VPg gene with the correct sequence. They generated VPg mRNA, but VPg protein was not detected. Some plants were immune to PVY infection suggesting post-transcriptional gene silencing. However, the likely PVY VPg toxicity exerted at an early stage of transformed seeds development precludes its use for engineering pathogen-derived resistance.
Źródło:
Acta Biochimica Polonica; 2011, 58, 3; 349-353
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Antibody response to DNA vaccine against H5N1 avian influenza virus in broilers immunized according to three schedules
Autorzy:
Stachyra, Anna
Góra-Sochacka, Anna
Zagórski-Ostoja, Włodzimierz
Król, Ewelina
Sirko, Agnieszka
Powiązania:
https://bibliotekanauki.pl/articles/1039267.pdf
Data publikacji:
2014
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Avian influenza
H5N1
DNA vaccine
broilers
Opis:
Broiler type chickens were immunized intramuscularly with a DNA vaccine encoding hemagglutinin (HA) from H5N1 avian influenza virus. The chickens were divided into four groups: control group which was not immunized, a group which obtained only one dose, and two groups which were immunized twice, one group with a boost two weeks after the priming and the other four weeks. Blood samples were collected at several time points and the dynamics of the humoral response to the vaccine was studied. High level of anti-HA antibodies was detected only in the last two groups, that is in chickens immunized according to the prime-boost strategy, regardless of the schedule. An additional interesting observation of this study was detection of the cross-reactivity of an anti-H5 HA positive serum with H5N2 and H1N1 viruses, suggesting that the DNA vaccine tested can induce antibodies of a broad specificity.
Źródło:
Acta Biochimica Polonica; 2014, 61, 3; 593-596
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Co-inoculation with two non-infectious cDNA copies of potato spindle tuber viroid (PSTVd) leads to the appearance of novel fully infectious variants.
Autorzy:
Podstolski, Wojciech
Góra-Sochacka, Anna
Zagórski, Włodzimierz
Powiązania:
https://bibliotekanauki.pl/articles/1041466.pdf
Data publikacji:
2005
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
non-coding RNA
viroid
circular RNA
PSTVd
recombination
Opis:
Potato spindle tuber viroid (PSTVd) is one of the smallest (about 360 nt) infectious plant agents. It is composed of a single-stranded circular non-coding RNA molecule. In the course of previous passage experiments with two intermediate PSTVd variants I2 and I4, three non-infectious clones (I2-50, I4-37 and I4 VI-17) were found. When inoculated separately as cDNAs on tomato "Rutgers" test plants these variants did not induce any visible disease symptoms and did not produce progeny. The presence of such non-infectious variants raises several questions about their origin and biology and to answer them, mixed co-infections with cDNA copies of two non-infectious variants (I2-50, I4-37) were performed. PSTVd infection was observed in seven out of 30 inoculated plants. The progeny isolated from three separate plants contained novel variants, together with the parental I2 and I4 sequences. It is conceivable that the appearance of repaired PSTVd molecules, clearly capable of cell-to-cell movement leading to the systemic infection, results from recombination events. An analysis of the recombinant molecules and comparison with databases identified the specific sites responsible for the restricted infectivity of the I2-50 and I4-37 PSTVd variants. In parallel experiments in which (+) strand PSTVd infectious transcripts were used, no recombinants were observed, and the original I2-50 and I4-37 non-infectious sequences were not detected in the progeny.
Źródło:
Acta Biochimica Polonica; 2005, 52, 1; 87-98
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Community cohesion, well-being, and local development
Autorzy:
Okrasa, Włodzimierz
Rozkrut, Dominik
Domański, Czesław
Zagórski, Krzysztof
Data publikacji:
2021-12
Wydawca:
Główny Urząd Statystyczny
Opis:
This publication features a collection of papers discussing the factors which have an impact on the quality of life and the subjective sense of well-being of local communities. The articles also provide empirical explanations as to what extent the developmental levels of the regions studied affect quality life and well-being. The publication is a product of the Community Cohesion and Well-Being and Innovative Local Development international scientific conference, organised jointly by the Institute of Sociology of the Cardinal Stefan Wyszyński University in Warsaw and Statistics Poland (held on 4 December 2018). The innovative character of this scientific event is mutually beneficial for its organisers. The joint initiative of the university and the main producer of statistical data deserves recognition, and any future project of this kind should be supported, as it provides a direct opportunity for the different potentials of both institutions to be brought together to discuss and explore advanced statistical, sociological and regional research. The 2018 conference was in fact its third edition, which indicates that the tradition of such a collaboration has already been established and gives hope to be upheld in the future. Despite the delay between the time of the conference and the publication date of the monograph, it still raises highly topical issues, and the presented analytical approaches combine the concern for scientific matters with policy objectives, assuming that the well-being of the citizens is the ultimate goal of community development. A wide spectrum of processes and their relevant factors, analysed at individual and community levels, embraces the elements of social, human and material capital along with innovative forms of their use by various agents, contributing to the cohesion and development of local communities. The variety of methodological approaches used by the authors makes their articles interesting and recommendable to all researchers and decision-makers, as well as to students of the subjects discussed in this publication.
Dostawca treści:
Biblioteka Nauki
Książka
Tytuł:
Cyrano de Bergerac
Autorzy:
Rostand, Edmond
Współwytwórcy:
Zagórski, Włodzimierz
Choromańska, Paulina
Konopnicka, Maria
Kotwica, Wojciech
Kopeć, Aleksandra
Data publikacji:
2019-09-12
Wydawca:
Fundacja Nowoczesna Polska
Tematy:
Komedia
Dramat
Modernizm
Opis:
Publikacja zrealizowana w ramach projektu Wolne Lektury (http://wolnelektury.pl). Dofinansowano ze środków Ministra Kultury i Dziedzictwa Narodowego.
Źródło:
Edmund Rostand, Cyrano de Bergerac, Nakładem Redakcyi "Gazety Polskiej", Warszawa 1898.
Dostawca treści:
Wolne Lektury
Książka
Tytuł:
Digoxigenin-labelled molecular probe for the simultaneous detection of three potato pathogens: potato spindle tuber viroid (PSTVd), potato virus Y (PVY), and potato leafroll virus (PLRV)
Autorzy:
Wełnicki, Marek
Żekanowski, Cezary
Zagórski, Włodzimierz
Powiązania:
https://bibliotekanauki.pl/articles/1045314.pdf
Data publikacji:
1994
Wydawca:
Polskie Towarzystwo Biochemiczne
Źródło:
Acta Biochimica Polonica; 1994, 41, 4; 473-475
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Elements regulating Potato leafroll virus sgRNA1 translation are located within the coding sequences of the coat protein and read-through domain
Autorzy:
Łoniewska-Lwowska, Adrianna
Chełstowska, Sylwia
Zagórski-Ostoja, Włodzimierz
Pałucha, Andrzej
Powiązania:
https://bibliotekanauki.pl/articles/1040473.pdf
Data publikacji:
2009
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
polerovirus
subgenomic RNA
in vitro translation
Opis:
Translation of viral proteins from subgenomic RNAs (sgRNAs) is a common strategy among positive-stranded RNA viruses. Unlike host mRNA, sgRNA of Potato leafroll virus (PLRV) does not possess a cap at its 5' end nor a poly(A) tail at the 3' terminus, both of which are known to be crucial for translation of RNA in eukaryotic cells. Here, we demonstrate, that in wheat germ extract (WGE) truncation of the sgRNA1 5' UTR increases translation efficiency, as it has previously been observed in rabbit reticulocyte lysate (RRL), whereas removal of the 3' UTR does not affect translation. We also describe two regulatory elements located within the coding sequence of the coat protein (CP) gene and its read-through domain (RTD) and are responsible for regulation of in vitro translation of the PLRV sgRNA1. The first element is composed of the purine sequence AAAGGAAA located between the AUG codons of the CP and 17K genes. Deletion of this domain or its substitution by pyrimidines reduced by half the translation of both genes, whereas deletion of the RTD resulted in a 3.6-fold reduction in translation efficiency. This is the first report of translation regulatory elements of plant viruses located within a coding region.
Źródło:
Acta Biochimica Polonica; 2009, 56, 4; 619-625
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Engineered resistance against proteinases
Autorzy:
Milner, Malgorzata
Chroboczek, Jadwiga
Zagorski-Ostoja, Wlodzimierz
Powiązania:
https://bibliotekanauki.pl/articles/1040935.pdf
Data publikacji:
2007
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
fusion proteins
proteinase inhibitors
protein protection
Opis:
Exogenous proteinase inhibitors are valuable and economically interesting protective biotechnological tools. We examined whether small proteinase inhibitors when fused to a selected target protein can protect the target from proteolytic degradation without simultaneously affecting the function and activity of the target domain. Two proteinase inhibitors were studied: a Kazal-type silk proteinase inhibitor (SPI2) from Galleria mellonella, and the Cucurbita maxima trypsin inhibitor I (CMTI I). Both inhibitors target serine proteinases, are small proteins with a compact structure stabilized by a network of disulfide bridges, and are expressed as free polypeptides in their natural surroundings. Four constructs were prepared: the gene for either of the inhibitors was ligated to the 5' end of the DNA encoding one or the other of two selected target proteins, the coat protein (CP) of Potato potyvirus Y or the Escherichia coli β-glucuronidase (GUS). CMTI I fused to the target proteins strongly hampered their functions. Moreover, the inhibitory activity of CMTI I was retained only when it was fused to the CP. In contrast, when fused to SPI2, specific features and functions of both target proteins were retained and the inhibitory activity of SPI2 was fully preserved. Measuring proteolysis in the presence or absence of either inhibitor, we demonstrated that proteinase inhibitors can protect target proteins used either free or as a fusion domain. Interestingly, their inhibitory efficiency was superior to that of a commercial inhibitor of serine proteinases, AEBSF.
Źródło:
Acta Biochimica Polonica; 2007, 54, 3; 523-536
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Expression of avian influenza haemagglutinin (H5) and chicken interleukin 2 (chIL-2) under control of the ptcB promoter in Lactococcus lactis
Autorzy:
Szatraj, Katarzyna
Szczepankowska, Agnieszka
Sączyńska, Violetta
Florys, Katarzyna
Gromadzka, Beata
Łepek, Krzysztof
Płucienniczak, Grażyna
Szewczyk, Bogusław
Zagórski-Ostoja, Włodzimierz
Bardowski, Jacek
Powiązania:
https://bibliotekanauki.pl/articles/1039270.pdf
Data publikacji:
2014
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Lactococcus lactis
ptcB promoter
heterologous gene expression
avian influenza H5N1
H5 haemagglutinin
chicken interleukin-2
Opis:
Gram-positive and nonpathogenic lactic acid bacteria (LAB) are considered to be promising candidates for the development of new, safe systems of heterologous protein expression. Recombinant LAB has been shown to induce specific local and systemic immune response against selected pathogens, and could be a good alternative to classical attenuated carriers. The main goal of our study was to express the avian influenza haemagglutinin (H5) and chicken interleukin 2 (chIL-2) in Lactococcus lactis. Results of this study were anticipated to lead to construction of lactococcal strain(s) with potential vaccine properties against the avian influenza A (H5N1) virus. Expression of the cloned H5 gene, its His-tagged variant and chIL-2 gene, under the control of the ptcB gene promoter was attested by RT-PCR on transcriptional level and Western or dot blot analysis on translational level, demonstrating that system can be an attractive solution for production of heterologous proteins. The results of the preliminary animal trial conducted in mice are a promising step toward development of a vaccine against avian bird flu using Lactococcus lactis cells as antigen carriers.
Źródło:
Acta Biochimica Polonica; 2014, 61, 3; 609-614
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Expression, purification and characterization of glycosylated influenza H5N1 hemagglutinin produced in Pichia pastoris
Autorzy:
Kopera, Edyta
Dwornyk, Angela
Kosson, Piotr
Florys, Katarzyna
Sączyńska, Violetta
Dębski, Janusz
Cecuda-Adamczewska, Violetta
Szewczyk, Bogusław
Zagórski-Ostoja, Włodzimierz
Grzelak, Krystyna
Powiązania:
https://bibliotekanauki.pl/articles/1039268.pdf
Data publikacji:
2014
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Avian influenza
H5N1
recombinant hemagglutinin
Pichia pastoris
Opis:
The A/swan/Poland/305-135V08/2006 (H5N1-subtype) hemagglutinin (HA) gene was cloned and expressed in yeast Pichia pastoris (P. pastoris). The HA cDNA lacking the C-terminal transmembrane anchor-coding sequence was fused to an α-factor leader peptide and placed under control of the methanol-inducible P. pastoris alcohol oxidase 1 (AOX1) promoter. Two P. pastoris strains: SMD 1168 and KM 71 were used for protein expression. Recombinant HA protein was secreted into the culture medium reaching an approximately 15 mg/L (KM 71 strain). Fusion protein with a His6 tag was purified to homogeneity in one step affinity chromatography. SDS-PAGE and MS/MS analysis indicated that the protein is cleaved into HA1 and HA2 domains linked by a disulfide bond. Analysis of the N-linked glycans revealed that the overexpressed HA is fully glycosylated at the same sites as the native HA in the vaccine strain. Immunological activity of the hemagglutinin protein was tested in mice, where rHA elicited a high immune response.
Źródło:
Acta Biochimica Polonica; 2014, 61, 3; 597-602
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Genetic variability of potato spindle tuber viroid RNA replicon.
Autorzy:
Góra-Sochacka, Anna
Candresse, Thierry
Zagórski, Włodzimierz
Powiązania:
https://bibliotekanauki.pl/articles/1044141.pdf
Data publikacji:
2001
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
genetic stability
sequence diversity
infectious clones
pathogenicity
potato spindle tuber viroid
Opis:
The genetic continuity of the potato spindle tuber viroid (PSTVd) genome was analysed after infection of tomato plants with cloned cDNAs of parental strains. During the six weeks of the experiment, several new sequence variants appeared. The sequence variants detected in the progeny population induced sequence-specific disease symptoms. The PSTVd genome therefore follows the pattern expected for typical pseudo-strains propagating in plants as a population of similar sequences. Assessing further the replicon continuity, a PSTVd cDNA mutant with a deletion in the central conserved region was constructed and proven to be non-infectious. Surprisingly, in a sub-population of potato transformants expressing the same deleted PSTVd RNA an infectious viroid was detected. This suggests specific transcript conversion followed by recovery of the full-length pathogen genome.
Źródło:
Acta Biochimica Polonica; 2001, 48, 2; 467-476
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 34

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