Wpływ sposobu przygotowania gleby na aktywność biologiczną gleby względem patogenów korzeni w 40-letnim drzewostanie sosnowym

Effects of pre−planting soil preparation on the clear−cut on the community structure of soil fungi and bacteria, their possible biological activity towards Armillaria and Heterobasidion, and mortality of Scots pine trees were studied in 40−year−old Scots pine plantation in Międzychód Forest District (W Poland). Pre−planting soil preparation included: (i) deep ploughing, (ii) shallow furrowing, (iii) making holes for planting, and (iv) shallow turning of the topsoil. The soil−dilution method was used for detection of fungi and bacteria in soil. Morphotyping was used for identification of fungi. Phenotypic traits and biochemical properties were used for identification of bacteria. Molecular method, MID−66 or BIOLOG® systems were additionally applied for identification of the most common bacteria. Deep furrowing, making holes for planting or shallow turning of the topsoil before planting increased abundance of fungi and bacteria in soil 40 years after treatment. Increased abundance of fungi and bacteria was associated with increased presence of taxa considered as antagonistic to Armillaria and Heterobasidion. The highest mortality of Scots pines was observed on sites with deep ploughing or shallow furrowing before planting, while the lowest mortality was found on sites with making holes for planting or shallow turning of the topsoil. The majority of dead trees were infected by H. annosum. Moderate intervention into the soil habitat on the clear−cut site before planting of Scots pine seedlings seems to create the habitat beneficial for the future growth of trees.