SELENO-L-METHIONINE MODULATE THE TOXIC EFFECT OF NEW DOXORUBICIN METAL COMPLEXES IN PROKARYOTIC MODEL –ESCHERICHIA COLI RFM443 RECA::LUX

Abstract: The main problem of modern cancer therapy is their resistance to the drugs used and the toxicity of drugs to healthy cells of the human body. Hope is natural substances that are protective of cells and often support the action of standard anticancer chemotherapy. Among them are selenium compounds. In the present study, we examined the cytotoxic and genotoxic potency of doxorubicin (DOX) metal complexes as new anticancer drug candidates. We also measured the effect of simultaneous administration of seleno –L-methionine as biologically active agent with DOX and its complexes with Mn, Mg, Fe, Co and Ni in prokaryotic model - Escherichia coli RFM443 with plasmid transcriptional fusion of recA promoter and luxCDABE as a reporter gene. The results obtained by us, indicate high biological activity of metal complexes with doxorubicin. The strongest modulatory effect was observed using the DOX complexes with Co and Ni compared to the DOX. The DOX complexes with Mg, Mn and Fe also showed relatively high biological activity. Simultaneous bacteria culture treatment with SeMet decreased the values of the DOX and its metal complexes cyto- and genotoxic parameters. These results encourage further research to assess the value of the doxorubicin metal complexes as new cytostatic drug candidates. While SeMet may be considered as therapeutic option in cancer treatment as modulation agent in anti-cancer therapy.