Wspolwystepowanie Borrelia garinii i B. afzelii wsrod populacji gryzoni lesnych

The maintenance of Borrelia burgdorferi s.1. in the environment is dependent on the zoonotic cycle involving tick vectors and certain reservoir hosts. It is well known, that the same species of wild rodents, as well as the vector Ixodes ricinus, are often co-infected with at least two genomospecies of B. burgdorferi s.1.: B. afzelii and B. garinii. The ticks collected from two rodent species: Clethrionomys glareolus and Apodemus flavicollis were examined for the presence of B. burgdorferi s.1., as well as for B. garinii and B. afzelii. In this study, an immunofluorescent antibody assay (lFA) and polymerase chain reaction (PCR) protocols were used. The high level of infestation in rodents (90% for C. glareolus and nearly 100% for A. flavicollis) shows that wild rodents are important hosts of the immature stages of I. ricinus. A high percent of Borrelia positive ticks collected from bank voles and yellow necked mice; above 7% determined by 1FA and 2% determined by PCR, elearly revealed that these species of animals are competent zoonotic reservoirs of B. burgdorferi s.l.