The effect of carbon and nitrogen sources and initial pH and temperature of the
medium on the mycelial growth of Sarcodon imbricatus (L.) P. Karst. in axenic
liquid culture was investigated. The optimal composition of the medium was found
to be: 5% fructose, 1% hydrolysate of casein, 1% yeast extract, and 0.3% KH2PO4 at
pH = 6 and incubation temperature of 20°C. In this condition the maximum biomass
growth was observed, yielding 10.2 g L−1 of dry weight after 3-week of growth.
The medium regarded as optimal for growth of S. imbricatus mycelium was used
for the production of the biomass and further chemical analysis. The quantitative
and qualitative composition of phenolic acids, fatty acids, and sterols were determined
using chromatographic methods. The total content of phenolic acids was
1.86 mg × 100 g−1 DW, with the largest amount of protocatechuic acid (1.27 mg ×
100 g−1 DW). Nineteen fatty acids were estimated, including five unsaturated fatty
acids, e.g., oleic and α-linolenic acid. The analysis of sterols composition revealed
the presence of ergosterol and ergosterol peroxide (197.7 and 200.47 mg × 100 g−1
DW, respectively). These compounds were isolated and confirmed by 1H-NMR.
Presented study constitutes the first report on the accumulation of substances
(phenolic acids, fatty acids, and sterols) with multidirectional biological activity in
the mycelial axenic culture of Sarcodon imbricatus.
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