Knowledge about molecular epidemiology of B. henselae is important for recognizing the geographical distribution of
strains and identification of isolates virulent for humans. Eleven Polish feline B. henselae isolates were typed, using 2 different
techniques: pulse-field gel electrophoresis (PFGE) and multiple-locus variable-number tandem repeat analysis (MLVA).
PFGE analysis distinguished 6 different PFGE types, with subtypes within 3 of them, whereas 10 MLVA types were assigned.
Global diversity index (D.I.) for MLVA equaled 0.93. For 7 isolates, the results of MLVA confirmed cluster assignments based
on PFGE. Both PFGE and MLVA results were in accordance with epidemiological data. Although PFGE has been previously
demonstrated to be a suitable method for the differentiation of B. henselae isolates/strains, our results show the superiority
of MLVA over PFGE with respect to higher discriminatory power, distinguishing genotypes I and II isolates, easier analysis of
results, and possibility to compare the numerical data obtained by different laboratories. With MLVA, 7 new profiles were
observed, compared to previous results from around the world; whereas 3 known profiles were previously described mainly
in European B. henselae isolates. Our results confirm that some VNTR profiles can be used as specific geographical markers.
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