Study of Anticonvulsant Clobazam Drug by Sophisticated Instrumentation

Clobazam (7-Chloro-1-methyl-5-phenyl-1,5-benzodiazepine-2,4(3H)-dione) an anticonvulsant drug used since 1984, with molecular formula C16H13ClN2O2 and molecular mass 300, was characterized with sophisticated instruments like FTIR, UV-NIR, GC-MS, TGA-DTA-DSC and Nano particle size analyzer with zeta potential. The compound was confirmed with the help of GC-MS and the mass of sample was found aggregate with the standard values and mass spectra compare with NIST and WELEY library data. The UV-NIR analysis showed that the λmax was 231 nm with methanol as a diluent. FTIR analysis of the Clobazam gave the characteristics peaks of cyclic amide. HPTLC was used to determine the purity of the drug. Thermal behavior was studied with TGA-DTA-DSC analysis. The particle size of clobazam was measured with the Nano particle size analyzer and Zeta potential of drud found -77.0 mV. The purity of drug check with Preparative HPLC system.