Effects of the platelet-activating factor (PAF) supplementation on ATP content of cryopreserved bull spermatozoa (AI)

The aim of this study was to investigate the effect of PAF supplementation in semen extender on ATP content in cryopreserved bull spermatozoa used for artificial insemination at different time intervals. Cryopreserved semen was treated with different concentrations of PAF: 1x10⁻⁵M, 1x10⁻⁶M, 1x10⁻⁷M, 1x10⁻⁸M and 1x10⁻⁹M at 37°C. In the present work we showed that content of ATP in cryopreserved semen supplemented with 1x10⁻⁹M PAF was statistically significantly higher at 90 and 120 minutes of incubation in comparison to the control group (p≤0.05). Present study indicates the potential influence of PAF on ATP content in male spermatozoa via it’s protective role towards mitochondria metabolic activity.