Tytuł pozycji:
The effect of experimental low zearalenone intoxication on ovarian follicles in pre-pubertal bitches
- Tytuł:
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The effect of experimental low zearalenone intoxication on ovarian follicles in pre-pubertal bitches
- Autorzy:
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Gajecka, M.
- Powiązania:
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https://bibliotekanauki.pl/articles/32335.pdf
- Data publikacji:
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2013
- Wydawca:
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Polska Akademia Nauk. Czytelnia Czasopism PAN
- Źródło:
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Polish Journal of Veterinary Sciences; 2013, 16, 1
1505-1773
- Język:
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angielski
- Prawa:
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CC BY-NC-ND: Creative Commons Uznanie autorstwa - Użycie niekomercyjne - Bez utworów zależnych 3.0 PL
- Dostawca treści:
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Biblioteka Nauki
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Przejdź do źródła  Link otwiera się w nowym oknie
Companion animals, including bitches, may be exposed to zearalenone (ZEN) toxins that are
often present in feed, and ZEN intoxication may lead to ovarian dysfunction. This study involved
evaluation of the degree of ZEN-induced hypo stimulation of ovary by determination of proliferative
and apoptotic indices and description of the ultra-structural organization of ovarian follicles in
pre-pubertal bitches subjected to experimental, long-term exposure to low-dose ZEN mycotoxicosis.
The experiment involved 30 clinically healthy, immature Beagle bitches aged approximately 70 days
with initial average body weight of 8 kg, randomly divided into three groups of 10 animals each: two
experimental groups (EI and EII) and a control group (C). Over a period of 42 days, ZEN was
administered per os to EI animals at a dose of 50 μg/kg BW, and to EII bitches at a dose of 75 μg/kg
BW. Control group animals were fed placebo containing no ZEN for 42 days. Analytical samples of
the mycotoxin were administered daily in gelatin capsules before morning feeding. All the bitches
were subjected to ovario-hysterectomy at the end of the experiment. Proliferation index (PCNA
method) and apoptotic index (TUNEL test) values were determined by immune-histological analyses.
The median for apoptotic index was determined at 13.45 for group EI, 17.84 for group EII, and 8.59
for group C. The median for proliferation index was determined at 35.25 for group EII, 42.44 for
group EI, and 70.60 for group C. The results of ultra-structural examinations of the ovaries revealed
that experimental, ZEN-induced hyperestrogenism enhanced apoptosis and lowered the proliferative
ability of follicular cells what contributed to organelle destruction in pre-pubertal bitches. The
changes observed were particularly advanced in EII animals, which were administered a higher dose
of ZEN.